Abstract：Objective To evaluate the safety of two types of enterovirus 71（EV71）inactivated vaccine（Vero cell vs. Human Diploid cell）after inoculation.Methods Subjects were children vaccinated EV71 vaccine between November 2016 and May 2017 at vaccination clinics in Xiacheng District. According to the type of EV71 vaccine，subjects were divided into the Vero Cell group and the Human Diploid Cell group. The adverse effects were collected after 30 min，3 days and 30 days of injection，respectively.Results A total of 3 158 vaccinated children were observed，with 1 529 in Vero Cell group and 1 629 in Human Diploid Cell group. The rates of adverse effects were 0.59%（9 cases）of Vero cell group and 0.55 %（9cases）of Human Diploid Cell group. All of the adverse effects were systemic reactions such as fever，diarrhea and vomiting. No significant differences were observed between the two groups above in the incidence and severity of adverse effects（ P>0.05）. The incidence and severity of adverse effects of the first dose were significantly higher than the second dose in two groups（P<0.05）.Conclusion EV71 vaccine was safe to vaccinate，and no statistical difference in the adverse effects was found between the two types of vaccine.