Abstract：Objective To investigate the contamination status and assess the potential consumption risk of Listeria monocytogenes ( L. monocytogenes ) in cooked meat products in bulk in Zhejiang Province, so as to provide strategy for food safety supervision and management. Methods A total of 2 320 cooked meat products were sampled from eleven cities in Zhejiang Province during 2018-2020. The detection of L. monocytogenes was carried out in accordance with the national standard GB/T 4789.30-2016. Risk Ranger software was used for the semi-quantitative risk assessment on the whole population and pregnant women. Results The total detection rate of L. monocytogenes in cooked meat products in bulk in Zhejiang Province was 2.97% ( 69/2 320 ). The detection rates in stewed, smoked/roasted, fried, dried products and others were 3.85%, 1.81%, 0.59%, 0% and 0.94%, which were significantly different ( P<0.05 ). There were 28 positive samples in 1 069 samples collected in 2020, with the concentration ranging from 5 to 590 CFU/g and averaging 6.8 CFU/g. The estimated number of listeriosis cases each year caused by consumption of cooked meat products in bulk was 131 in the whole population with a risk score of 42, and 1.44 in pregnant women with a risk score of 54. The risk coefficient could reduce to approximate zero after sufficient heating before intake. Conclusion The prevalence of L. monocytogenes in cooked meat products in bulk in Zhejiang Province during 2018-2020 poses a potential risk in food safety. Pregnant women should avoid eating.
[1] World Health Organization.Listeriosis[EB/OL].(2018-02-20)[2021-04-19].https://www.who.int/news-room/fact-sheets/detail/listeriosis. [2] LOMONACO S,NUCERA D,FILIPELLO V.The evolution and epidemiology of Listeria monocytogenes in Europe and the United States[J].Infect Genet Evol,2015,35:172-183. [3] RICCHI M,SCALTRITI E,CAMM G,et al.Short communication:persistent contamination by Listeria monocytogenes of bovine raw milk investigated by whole-genome sequencing[J].J Dairy Sci,2019,102(7):6032-6036. [4] 宋筱瑜,裴晓燕,徐海滨,等.我国零售食品单核细胞增生李斯特氏菌污染的健康风险分级研究[J].中国食品卫生杂志,2015,27(4):447-450. [5] LI W,BAI L,FU P,et al.The epidemiology of Listeria monocytogenes in China[J]. Foodborne Pathog Dis,2018,15(8):459-466. [6] Food and Drug Administration/Food Safety Inspection Service. Quantitative assessment of relative risk to public health from foodborne Listeria monocytogenes among selected categories of ready-to-eat foods[S/OL].[2021-04-19]. https://www.fda.gov/media/124721/download. [7] 田明胜,王颖,陈波,等.上海市售肉制品中单核细胞增生李斯特氏菌污染监测和定量分析[J]. 生物加工过程,2020,18(3):392-396. [8] 中华人民共和国国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准食品微生物学检验单核细胞增生李斯特氏菌检验:GB 4789.30—2016[S].2016. [9] ROSS T,SUMNER J.A simple,spread-sheet based,food safety risk assessment tool[J]. Int J Food Microbiol,2002,77(1/2): 39-53. [10] 蔡志强,杨菊艳,蒋小燕.单核细胞增生李斯特氏菌感染的临床特征[J].中国感染控制杂志,2020,19(10):900-903. [11] World Health Organization.Risk assessment of Listeria monocytogenes in ready-to-eat foods: interpretative summary[DB/OL]. [2021-04-19].https://apps.who.int/iris/bitstream/handle/10665/42874/9241562617.pdf?sequence=1&isAllowed=y. [12] TODD E C D,NOTERMANS S. Surveillance of listeriosis and its causative pathogen,Listeria monocytogenes[J]. Food Control,2011,22(9):1484-1490. [13] 王志宏,孙静,王惠君,等. 中国居民膳食结构的变迁与营养干预策略发展[J]. 营养学报,2019,41(5):427-432. [14] 李同斌. 中国肉类加工业的发展机遇和挑战[J]. 肉类工业,2005(7):40-42. [15] 刘海霞,许燕,杨祖顺,等. 2010—2016年云南省熟肉制品和餐饮食品中单增李斯特菌污染情况调查分析[J].食品安全质量检测学报,2017,8(10):3768-3772. [16] 翟明爽,徐斐,曹慧,等.即食熟肉制品中主要致病菌的半定量风险评估[J].微生物学杂志,2014,34(2):92-98. [17] 文涛,王文思,孙葳,等.辽宁省食品中单增李斯特菌监测分析[J].中国食品卫生杂志,2015,31(11):1475-1477. [18] VAN DER FELS-KLERX H J,VAN ASSELT E D,RALEY M,et al. Critical review of methods for risk ranking of food-related hazards,based on risks for human health[J]. Crit Rev Food Sci Nutr,2018,58(2):178-193. [19] 魏星,罗棵濒,罗丽,等. 成都市售食品及加工环节金黄色葡萄球菌的污染状况及半定量风险评估[J]. 卫生研究,2020,49(4):608-610.
