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| A case-control study on the association between single nucleotide polymorphism of eNOS gene T-786C and G894T and the risk of coronary heart disease |
| ZHU Na, HU Jin-fei
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| Jinghua Central Hospital, Jinhua, Zhejiang, 321037, China |
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Abstract ObjectiveTo explore the association between single nucleotide polymorphism(SNPs) of eNOS gene T-786C and G894T and the risk of coronary heart disease (CHD). MethodsGenotypes of single nucleotide polymorphism(SNPs) of eNOS gene T-786C and G894T were detected in 842 CHD patients and 842 age and gender matched healthy control individuals using Taqman-polymerase chain reaction genotyping assay and the association between them were analyzed.ResultsGenotype distributions of the two loci in controls were in consistence with Hardy-Weinberg inheritance model. Frequencies of allele C (8.79% vs. 6.53%, P=0.014, OR=2.54, 95%CI=2.01-3.14), CC (0.84% vs. 0.11%, P=0.04, OR=3.81, 95%CI=1.74-5.56) and TC/CC (16.75% vs. 13.06%, P=0.03,OR=2.11, 95%CI=1.75-2.98) genotypes in cases were obviously higher than in controls. However, there was no significant frequency difference of TC genotype in two groups. No significant association was found between allele T, genotype GT, TT and GT/TT and the risk of CHD. ConclusionAllele C, genotype CC and TC/CC of T-786C within eNOS gene were statistically significantly associated with increased risk of CHD, indicating that this locus might be involved in the pathological process of CHD, and allele C, genotype CC, TC/CC might be genetic susceptible factors for CHD.
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Received: 16 April 2015
Revised: 04 August 2015
Published: 05 January 2018
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