Abstract:Objective To examine the correlation between exposure to polycyclic aromatic hydrocarbons ( PAHs ) and placental mitochondrial DNA ( mtDNA ) copy number among pregnant women, so as to provide the evidence for evaluation of potential effects of PAHs exposure during pregnancy on offspring health. Methods A total of 200 pregnant women delivered at a tertiary hospital in Urumqi City during the period from January to October 2019, and their newborns were recruited, and grouped according to the time of delivery, including the heating group [delivery during the heating period ( from January to April ) ] and the non-heating group [delivery during the non-heating period (from July to October) ]. Subjects'age, ethnicity, educational level and type of home heating were collected, and the total concentration of 16 PAHs was determined in the blood samples of pregnant women and their babies using gas chromatography-mass spectrometry. Placental DNA was extracted, and placental mtDNA copy number was measured using real-time fluorescence quantitative PCR assay. In addition, the correlation between PAHs concentration and placental mtDNA copy number was examined using the Spearman rank correlation analysis. Results There were 100 subjects in the heating group, which had a median age of 29 ( interquartile range, 3 ) years and had a mean gestational age of ( 275.06±0.72 ) days, and there were 100 subjects in the non-heating group, which had a median age of 29 ( interquartile range, 4 ) years and had a mean gestational age of ( 276.82±0.66 ) days. The total concentration of PAHs in the blood of pregnant women [15.71 (4.30) vs. 12.98 (5.49) μg/L; P<0.05 ], the total concentration of PAHs in neonatal blood [ 14.29 (4.25) vs. 11.24 (5.09) μg/L; P<0.05 ] and the placental mtDNA copy number [4.67 (1.18) vs. 4.51 (0.62); P<0.05] were all higher in the heating group than in the non-heating group. Spearman rank correlation analysis revealed that the total concentration of PAHs in the blood of pregnant women and neonates was positively correlated with placental mtDNA copy number ( rs=0.240, P=0.001; rs=0.273, P<0.001 ), and the total concentration of PAHs in the blood of pregnant women was positively correlated with the placental mtDNA copy number in the heating group ( rs=0.245, P=0.014 ), while the PAHs concentration in the neonatal blood was positively correlated with the placental mtDNA copy number in the non-heating group ( rs=0.292, P=0.003 ). Conclusions Exposure to PAHs positively correlates with placental mtDNA copy number among pregnant women, and there is a correlation between maternal exposure to PAHs and neonatal oxidative stress.
[1] 李琼,陈燊,张海燕,等.利用Tox21通路数据库评估石家庄市大气细颗粒物中多环芳烃激活毒性通路的风险[J].癌变·畸变·突变,2020,32(2):112-117. LI Q,CHEN S,ZHANG H Y,et al.Risk assessment of fine particulate matter exposure using toxicity pathways of human lung cells[J].Carcinog Teratog Mutagen,2020,32(2):112-117. [2] PAVANELLO S,DIONI L,HOXHA M,et al.Mitochondrial DNA copy number and exposure to polycyclic aromatic hydrocarbons[J].Cancer Epidemiol Biomarkers Prev,2013,22(10),1722-1729. [3] LING X,ZHANG G W,SUN L,et al.Polycyclic aromatic hydrocarbons exposure decreased sperm mitochondrial DNA copy number:a cross-sectional study(MARHCS)in Chongqing,China[J].Environ Pollut,2017,220(Pt A):680-687. [4] GENKINGER J M,STIGTER L,EDRYCHOWSKI W,et al.Prenatal polycyclic aromatic hydrocarbon(PAH)exposure,antioxidant levels and behavioral development of children ages 6-9[J].Environ Res,2015,140(7):136-144. [5] 梁轶群,张风华.多环芳烃对儿童早期发育的神经毒性效应分析[J].中国妇幼保健,2016,31(23):5217-5219. LIANG Y Q,ZHANG F H.Analysis of neurotoxic effects of polycyclic aromatic hydrocarbons on early development of children[J].Matern Child Health Care China,2016,31(23):5217-5219. [6] 韩瑜. 乳腺癌患者体内多环芳烃代谢水平研究[D].乌鲁木齐:新疆医科大学,2013. HAN Y.The study of the polycyclic aromatic hydrocarbon metabolites in breast cancer patients[D].Urumqi:Xinjiang Medical University,2013. [7] 张露艺,李欣,鲁英.乌鲁木齐市母婴多环芳烃负荷水平及关系:基于某三甲医院的调查[J].环境与职业医学,2020,37(10):963-969. ZHANG L Y,LI X,LU Y.Relationship between polycyclic aromatic hydrocarbons loads of pregnant women and newborns in a Class-A teritary hospital in Urumqi[J].J Environ Occup Med,2020,37(10):963-969. [8] 汪启兵. 多环芳烃在母胎间的传输及其与病理妊娠的研究[D].昆明:昆明理工大学,2017. WANG Q B.The transmission of polycyclic aromatic hydrocarbons between mother and fetus and its relationship with pathological pregnancy[D].Kunming:Kunming University of Science and Technology,2017. [9] 杨立新,曾凡刚,张晓娜,等.人血液中16中多环芳烃测定的固相萃取结合气相色谱-质谱法[J].中华劳动卫生职业病杂志,2019,37(2):139-143. YANG L X,ZENG F G,ZHANG X N,et al.Solid phase extraction binding gas chromatography-mass spectrometry measured by polycyclic aromatic hydrocarbons in human blood[J].Chin J Ind Hyg Occup Dis,2019,37(2):139-143. [10] 陈小兰,刘晓红,霍文华,等.北京地区母体血清多环芳烃水平与自发性早产发生风险的关联性[J].中国生育健康杂志,2017,28(1):1-5,9. CHEN X L,LIU X H,HUO W H,et al.Association of polycyclic aromatic hydrocarbons in maternal plasma with risk of spontaneous preterm birth[J].Chin J Reprod Health,2017,28(1):1-5,9. [11] 周涛,张建中.乌鲁木齐市采暖期供暖燃气锅炉现状分析及对策[J].资源节约与环保,2018(10):20-21. ZHOU T,ZHANG J Z.Current situation analysis and countermeasures of heating gas boilers in Urumqi[J].Resour Econ Environ Prot,2018(10):20-21.