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预防医学  2022, Vol. 34 Issue (3): 248-252    DOI: 10.19485/j.cnki.issn2096-5087.2022.03.007
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孕期PAHs暴露与胎盘线粒体拷贝数的关联研究
朱玲慧1, 鲁英2, 张露艺1
1.新疆医科大学公共卫生学院,新疆 乌鲁木齐 830054;
2.海南医学院,海南 海口 571199
Correlation between exposure to polycyclic aromatic hydrocarbons during pregnancy and placental mitochondrial DNA copy number
ZHU Linghui1, LU Ying2, ZHANG Luyi1
1. School of Public Health, Xinjiang Medical University, Urumqi, Xinjiang 830054, China;
2. Hainan Medical College, Haikou, Hainan, 571199, China
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摘要 目的 分析孕妇多环芳烃(PAHs)暴露水平与胎盘线粒体DNA(mtDNA)拷贝数的相关性,为孕期PAHs暴露对子代健康潜在影响研究提供依据。方法 选择2019年1—10月在乌鲁木齐市某三甲医院分娩的200名孕妇及其新生儿为研究对象,根据分娩时间分为采暖期组(1—4月)和非采暖期组(7—10月),收集年龄、民族、文化程度和家庭采暖方式等资料;采用气相色谱-质谱联用法测定孕妇及其新生儿血液16种PAHs总浓度;提取胎盘DNA,采用实时荧光定量PCR法检测胎盘mtDNA拷贝数;采用Spearman秩相关分析血PAHs总浓度与胎盘mtDNA拷贝数的相关性。结果 纳入采暖期组和非采暖期组各100人,年龄分别为29(3)岁和29(4)岁,孕期分别为(275.06±0.72)和(276.82±0.66)d。采暖期组孕妇血PAHs总浓度[15.71(4.30)vs. 12.98(5.49)μg/L]、新生儿血PAHs总浓度[14.29(4.25)vs. 11.24(5.09)μg/L]和胎盘mtDNA拷贝数[4.67(1.18)vs. 4.51(0.62)]均高于非采暖期组(P<0.05)。孕妇和新生儿血PAHs总浓度均与胎盘mtDNA拷贝数呈正相关(rs=0.240,P=0.001;rs=0.273,P<0.001)。采暖期组孕妇血PAHs总浓度与胎盘mtDNA拷贝数呈正相关(rs=0.245,P=0.014);非采暖期组新生儿血PAHs浓度与胎盘mtDNA拷贝数呈正相关(rs=0.292,P=0.003)。结论 孕妇PAHs暴露水平与胎盘mtDNA拷贝数呈正相关,孕妇PAHs暴露与新生儿氧化应激存在一定相关性。
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朱玲慧
鲁英
张露艺
关键词 孕妇新生儿多环芳烃mtDNA拷贝数氧化应激    
AbstractObjective To examine the correlation between exposure to polycyclic aromatic hydrocarbons ( PAHs ) and placental mitochondrial DNA ( mtDNA ) copy number among pregnant women, so as to provide the evidence for evaluation of potential effects of PAHs exposure during pregnancy on offspring health. Methods A total of 200 pregnant women delivered at a tertiary hospital in Urumqi City during the period from January to October 2019, and their newborns were recruited, and grouped according to the time of delivery, including the heating group [delivery during the heating period ( from January to April ) ] and the non-heating group [delivery during the non-heating period (from July to October) ]. Subjects'age, ethnicity, educational level and type of home heating were collected, and the total concentration of 16 PAHs was determined in the blood samples of pregnant women and their babies using gas chromatography-mass spectrometry. Placental DNA was extracted, and placental mtDNA copy number was measured using real-time fluorescence quantitative PCR assay. In addition, the correlation between PAHs concentration and placental mtDNA copy number was examined using the Spearman rank correlation analysis. Results There were 100 subjects in the heating group, which had a median age of 29 ( interquartile range, 3 ) years and had a mean gestational age of ( 275.06±0.72 ) days, and there were 100 subjects in the non-heating group, which had a median age of 29 ( interquartile range, 4 ) years and had a mean gestational age of ( 276.82±0.66 ) days. The total concentration of PAHs in the blood of pregnant women [15.71 (4.30) vs. 12.98 (5.49) μg/L; P<0.05 ], the total concentration of PAHs in neonatal blood [ 14.29 (4.25) vs. 11.24 (5.09) μg/L; P<0.05 ] and the placental mtDNA copy number [4.67 (1.18) vs. 4.51 (0.62); P<0.05] were all higher in the heating group than in the non-heating group. Spearman rank correlation analysis revealed that the total concentration of PAHs in the blood of pregnant women and neonates was positively correlated with placental mtDNA copy number ( rs=0.240, P=0.001; rs=0.273, P<0.001 ), and the total concentration of PAHs in the blood of pregnant women was positively correlated with the placental mtDNA copy number in the heating group ( rs=0.245, P=0.014 ), while the PAHs concentration in the neonatal blood was positively correlated with the placental mtDNA copy number in the non-heating group ( rs=0.292, P=0.003 ). Conclusions Exposure to PAHs positively correlates with placental mtDNA copy number among pregnant women, and there is a correlation between maternal exposure to PAHs and neonatal oxidative stress.
Key wordspregnant woman    newborn    polycyclic aromatic hydrocarbons    mtDNA copy number    oxidative stress
收稿日期: 2021-11-29      修回日期: 2022-01-28      出版日期: 2022-03-10
中图分类号:  R172  
  R181.3  
基金资助:国家自然科学基金项目(81960580)
通信作者: 鲁英,E-mail:316139862@qq.com   
作者简介: 朱玲慧,硕士在读
引用本文:   
朱玲慧, 鲁英, 张露艺. 孕期PAHs暴露与胎盘线粒体拷贝数的关联研究[J]. 预防医学, 2022, 34(3): 248-252.
ZHU Linghui, LU Ying, ZHANG Luyi. Correlation between exposure to polycyclic aromatic hydrocarbons during pregnancy and placental mitochondrial DNA copy number. Preventive Medicine, 2022, 34(3): 248-252.
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http://www.zjyfyxzz.com/CN/10.19485/j.cnki.issn2096-5087.2022.03.007      或      http://www.zjyfyxzz.com/CN/Y2022/V34/I3/248
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