低浓度苯暴露与外周血淋巴细胞miR-223和miR-155表达的关联研究

doi:10.19485/j.cnki.issn2096-5087.2022.01.003

Preventive Medicine

2022, Vol. 34

Issue (1): 11-16 DOI: 10.19485/j.cnki.issn2096-5087.2022.01.003

Original Articles

Current Issue | Archive | Adv Search

Effect of exposure to low concentrations of benzene on miR-223 and miR-155 expression in peripheral blood lymphocytes

WANG Aihong^1,2, LI Xiaohai^1,2, LENG Pengbo^1,2, DUAN Donghui^1,2, FANG Lanyun^1,2, ZHANG Dandan^1,2

1. Department of Environmental and Occupational Health, Ningbo Center for Disease Control and Prevention,Ningbo, Zhejiang 315010, China;
2. Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo, Zhejiang 315010, China

Abstract
Figure/Table
References
Related Citation (1)

Download: PDF (835 KB) HTML (0 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To investigate the effect of exposure to low concentrations of benzene on miR-155 and miR-223 expression in peripheral blood lymphocytes among workers with benzene exposure. Methods A hundred male employees at a risk of exposure to benzene (the exposed group) were randomly sampled from two small metal products manufacturing enterprises and one medium-sized chemical raw material and chemical products manufacturing enterprise in Ningbo City, Zhejiang Province, and 60 age-matched male employees without benzene exposure were randomly selected as the unexposed group. Age, body mass index ( BMI ), smoking status, alcohol consumption, disease history, medication history and routine blood testing results of subjects were collected using a questionnaire survey. The 8-hour time weighted average concentration ( C_TWA ) of benzene was measured in the workplace using thermal desorption gas chromatography, and the urine 8-hydroxy-2' deoxyguanosine ( 8-OHdG ) levels were determined using high-performance liquid-chromatography tandem mass spectrometry (HPLC-MS/MS). The miR-155 and miR-223 expression was quantified in peripheral blood lymphocytes using quantitative fluorescent reverse transcription-polymerase chain reaction assay, and the factors affecting miR-155 and miR-223 expression were identified using multivariable logistic regression analysis. Results The subjects in the exposed group had a mean age of ( 31.17±7.30 ) years, and were exposed to low concentrations of benzene ( C_TWA, 0.05 to 0.30 mg/m³ ) , while the subjects in the unexposed group had a mean age of ( 32.52±6.15 ) years. There were no significant differences between the exposed and unexposed groups in terms of age, BMI, proportion of smokers or proportion of alcohol consumers ( P>0.05 ). There was no significant difference in the median relative miR-155 expression between the exposed and unexposed groups ( 0.953 vs. 1.293, P>0.05 ), and lower median relative miR-223 expression was quantified in the exposed group than in the unexposed group ( 0.540 vs. 1.433, P<0.05 ). Multivariable logistic regression analysis revealed that down-regulation of miR-223 expression correlated with exposure to benzene ( OR=2.719, 95%CI: 1.308-5.651 ). Conclusion Down-regulation of miR-223 expression may be associated with exposure to low concentrations of benzene.

Key words： benzene exposure microRNA peripheral blood lymphocyte oxidative damage

Received: 03 September 2021 Revised: 01 December 2021 Published: 12 January 2022

ZTFLH:

R134

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WANG Aihong
	LI Xiaohai
	LENG Pengbo
	DUAN Donghui
	FANG Lanyun
	ZHANG Dandan

Cite this article:

WANG Aihong,LI Xiaohai,LENG Pengbo, et al. Effect of exposure to low concentrations of benzene on miR-223 and miR-155 expression in peripheral blood lymphocytes[J]. Preventive Medicine, 2022, 34(1): 11-16.

URL:

http://www.zjyfyxzz.com/EN/10.19485/j.cnki.issn2096-5087.2022.01.003 OR http://www.zjyfyxzz.com/EN/Y2022/V34/I1/11

[1] SCHNATTER A R,KERZIC P J,ZHOU Y,et al.Peripheral blood effectsin benzene-exposed workers[J].Chem Biol Interact,2010,184(1/2):174-181.
[2] VIANNA N J,POLAN A.Lymphomas and occupational benzene exposure[J].Lancet,1979,313(8131):1394-1395.
[3] 刘洋,张恒东,陈献文,等.苯作业工人血浆差异表达微小RNA的初步筛选与分析[J].中华劳动卫生职业病杂志,2014,32(7):511-515.
LIU Y,ZHANG H D,CHEN X W,et al.Screening and analysis of plasma microRNA profile in benzene exposed workers[J].Chin J Ind Hyg Occup Dis,2014,32(7):511-515.
[4] BAI W L,CHEN Y J,YANG J,et al.Aberrant miRNA profiles associated with chronic benzene poisoning[J].Exp Mol Pathol,2014,96(3):426-430.
[5] 中华人民共和国卫生部.血细胞分析参考区间:WS/T 405—2012[S].2012.
Ministry of Health of the People's Republic of China.Reference intervals for blood cell analysis:WS/T 405-2012[S].2012.
[6] 中华人民共和国卫生部.工作场所空气中有害物质监测的采样规范:GBZ 159—2004[S].北京:人民卫生出版社,2004.
Ministry of Health of the People's Republic of China.Specifications of air sampling for hazardous substances monitoring in the workplace:GBZ 159-2004[S].Beijing:People's Medical Publishing House,2004.
[7] 中华人民共和国国家卫生和计划生育委员会.工作场所空气有毒物质测定第66部分:苯、甲苯、二甲苯和乙苯:GBZ/T 300.66—2017[S].2017.
National Health and Family Planning Commission of the People's
Republic of China.Determination of toxic substances in workplace air-Part 66:benzene,toluene,xylene and ethyl benzene:GBZ/T 300.66-2017[S].2017.
[8] 王爱红,李晓海,冷朋波,等.低浓度苯暴露男性工人尿8-OHdG水平的影响因素[J].环境与职业医学,2020,37(3):243-248.
WANG A H,LI X H,LENG P B,et al.Influencing factors of urinary 8-OHdG concentration in male workers exposed to low levels of benzene[J].J Environ Occup Med,2020,37(3):243-248.
[9] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2^-△△Ct method[J].Methods,2001,25:402-408.
[10] 朱丹霞,缪扣荣,朱远东,等.实时定量PCR检测白血病相关miRNA方法的建立[J].中国实验血液学杂志,2010,18(3):756-761.
ZHU D X,MIAO K R,ZHU Y D,et al.Detection of miRNA levels in leukemia patients by real-time quantitative PCR[J].J Exp Hematol,2010,18(3):756-761.
[11] 王秋艳,武志云,张岩,等.低浓度苯及混苯对接触工人血常规及淋巴细胞微核的影响[J].河北医药,2020,42(8):1250-1253.
WANG Q Y,WU Z Y,ZHANG Y,et al.Effects of low concentration benzene on blood routine count and lymphocyte micronucleus in exposed workers[J].Hebei Med J,2020,42(8):1250-1253.
[12] 彭艳,张磊,朱媛媛,等.杭州市苯接触作业在岗工人血常规异常及影响因素分析[J].预防医学,2020,32(10):1059-1061.
PENG Y,ZHANG L,ZHU Y Y,et al.Abnormality in routine blood tests and its influencing factors among workers exposed to benzene in Hangzhou[J].Prev Med,2020,32(10):1059-1061.
[13] KIM J H,MOON J Y,PARK E Y,et al.Changes in oxidative stress biomarker and gene expression levels in workers exposed to volatile organic compounds[J].Ind Health,2011,49(1):8-14.
[14] LIU Y,CHEN X W,NIAN Q,et al.Analysis of plasma microRNA expression profiles in a Chinese population occupationally exposed to benzene and in a population with chronic benzene poisoning[J].J Thorac Dis,2016,8(3):403-414.
[15] WEI H Y,ZHANG J,TAN K H,et al.Benzene-induced aberrant miRNA expression rofile in hematopoietic progenitor cells in C57BL/6 mice[J].Int J Mol Sci,2015,16(11):27058-27071.
[16] 叶伟国,黄浩宇,姜燃,等.氢醌处理人支气管上皮细胞(16HBE)对其miR-221及抑癌基因PTENmRNA表达的影响[J].毒理学杂志,2019,33(3):226-229.
[17] BISWAS R,MAHESH G.MicroRNA-155:a master regulator of inflammation[J].J Interferon Cytokine Res,2019,39(6):321-330.
[18] JOHNNIDIS J B,HARRIS M H,WHEELER R T,et al.Regulation of progenitor cell proliferation and granulocyte function by microRNA-223[J].Nature,2008,451(7182):1125-1129.
[19] AOKI H,TANI H,NAKAMURA K,et al.MicroRNA biomarkers for chemical hazard screening identified by RNA deep sequencing analysis in mouse embryonic stem cells[J/OL].Toxicol Appl Pharmacol,2020,392[2021-12-01].https://doi.org/10.1016/j.taap.2020.114929.
[20] 王爱红,李晓海,冷朋波.苯毒性作用机制及其与miRNA关系的研究进展[J].中华劳动卫生职业病杂志,2018,36(3):237-240.
WANG A H,LI X H,LENG P B.Research progress on toxicity mechanism of benzene and its relationship with miRNA[J].Chin J Ind Hyg Occup Dis,2018,36(3):237-240.