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| Determination of lead in blood by matrix-matched calibration curve correction-inductively coupled plasma mass spectrometry |
| XU Yan, ZHANG Xuejie, DU Pengcheng
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| Physical and Chemical Laboratory, Detection Section, Kaifeng Center for Disease Control and Prevention, Kaifeng, Henan 475000, China |
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Abstract Objective To develop the matrix-matched calibration curve correction-inductively coupled plasma mass spectrometry (ICP-MS) for the determination of lead in blood. Methods Whole blood samples and blank whole blood were pretreated by direct dilution with a solution of 0.5% nitric acid and 0.01% TritonX-100 to obtain whole blood sample solutions and matrix-matched solvents at a 10-fold dilution. The mass concentration of lead was determined by using an ICP-MS instrument in He mode. 175Lu was added online as an internal standard. The standard working curve was calibrated with the matrix-matched solvent, and the mass concentration of lead in the whole blood samples was calculated based on the standard working curve. Recovery tests were performed on whole blood blind samples by spiking, and the relative standard deviation and average recovery rate were calculated. The accuracy and precision of this method were assessed by comparing it with the method recommended in the national standard in detection of lead in three types of bovine blood lead standard materials. Results Good linearity was shown for lead at 0.5 to 100.0 μg/L, with a correlation coefficient of 1.000. The detection limit of lead was 0.4 μg/L, and the quantitation limit was 1.3 μg/L. The relative standard deviations were 0.65% and 1.10%. The average recovery ranged from 96.89% to 99.73%. The lead determination results were all within the normal reference ranges specified by the three certified reference materials for bovine blood samples. Conclusion The matrix-matched calibration curve correction-ICP-MS is suitable for high-throughput determination of blood lead.
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Received: 29 July 2024
Revised: 15 November 2024
Published: 13 December 2024
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[1] 刘玉波,周怡兵,黄江,等.微波消解-石墨炉原子吸收光谱法测定血和尿中的铅和镉[J].现代预防医学,2017,44(10):1859-1861,1866.
[2] 张淼,郑磊,孙琦,等.电感耦合等离子体质谱法测定血铅实验影响因素[J].卫生研究,2023,52(1):136-141.
[3] 中华人民共和国国家卫生健康委员会.中华人民共和国国家职业卫生标准血中铅的测定:GBZ/T 316—2018[S].北京:中国标准出版社,2022.
[4] 杨丽,张雪杰,胥艳.逆王水-氢氟酸混合体系消解-电感耦合等离子体质谱法同时测定土壤中Pb、Cr和Cd[J].预防医学,2021,33(1):104-106.
[5] 应英,王立媛,虞晓珍,等.电感耦合等离子体质谱法和石墨炉原子吸收光谱法测定冻干血铬比较[J].预防医学,2019,31(7):751-753.
[6] 张淼,郑磊,丁亮.全血中13种元素的电感耦合等离子体质谱快速测定法[J].环境化学,2020,39(9):2421-2429.
[7] 中华人民共和国国家质量监督检验检疫总局,国家标准化管理委员会.实验室质量控制规范食品理化检测:GB/T 27404—2008[S].北京:中国标准出版社,2008.
[8] 王树加,郑巧清,卢思,等. 微波消解-电感耦合等离子体质谱(ICP-MS)法测定土壤中7种金属元素[J].中国无机分析化学,2023,13(12):1336-1341.
[9] 黄子敬,陈孟君,邓华阳,等. 微波消解-ICP-MS混合模式测定动植物源食品中 11 种金属元素[J].分析试验室,2017,36(1):24-28. |
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