|
|
|
| Optimization of chromatographic conditions for the determination of clenbuterol hydrochloride residues in animal edible product |
| CAO Yi-yao，YU Shun-fei，XUAN Zhi-qiang，ZHAO Yao-xian，LI Xin-xing，WU Shou-ming，ZHAO San-hu，LIU Ping
|
| *Zhejiang Provincial Center for Disease Control and Prevention，Hangzhou，Zhejiang 310051，China |
|
|
|
|
Abstract Objective To establish a high performance liquid chromatography（HPLC）through the optimization of the chromatographic conditions，which can detect the contents of clenbuterol hydrochloride（CL）residues in animal edible product in a large quantity. Methods The animal edible product were extracted by perchloric acid，and then impurities were removed by liquid-liquid extraction（LLE）which used ethyl acetate- isopropanol. After the organic phase was concentrated，C18 column（150 mm×4.6 mm，5 μm）was used to separate CL. Mobile phase were methanol-sodium dihydrogen phosphate，and then determined by HPLC. Results A good linear response was obtained over the range of 0.2-10.0 μg/mL with the correlation coefficient（r）0.999 84. The method determination limit was 0.15 μg/kg which was lower than the National standard method 0.5 μg/kg. The retention time of the CL was 6.51 min，the chromatographic peak was good. The recovery rates spiked with standards 1.6-12 μg were 92.86%-100.93%，which was higher than National standard method（89.79%-92.36%）. The precision of intra-day and inter-day were both under 5%，which lower than National standard. Conclusion The optimized chromatographic conditions are suitable for the large quantity determination of clenbuterol hydrochloride in animal edible product.
|
|
Published: 04 June 2018
|
|
|
|
|
|
[1] 曹巧玲，王中民，秦珑，等. 盐酸克伦特罗的毒性、作用机制及其检测方法研究进展[J] . 职业与健康，2011，27（20）：2376-2378.
[2] KIM H K，DELLA-FERA M A，HAUSMAN D B，et al. Effect of clenbuterol on apoptosis，adipogenesis，and lipolysis in adipocytes[J] . J Physiol Biochem，2010，66（3）：197-203.
[3] ZHANG Q，XIANG J，WANG X，et al. Β2-adrenoceptor agonist clenbuterol reduces infarct size and myocardial apoptosis after myocardial ischaemia/reperfusion in anaesthetized rats[J] . British Journal of Pharmacology，2010，160（6）：1561-1572.
[4] 黎源倩. 食品理化检验[M] . 北京：人民卫生出版社，2009： 114-116.
[5] 中华人民共和国卫生部，中国国家标准化管理委员会. 动物性食品中克仑特罗残留量的测定：GB/T 5009.192—2003[S] . 北京：中国标准出版社，2003.
[6] 吴庆洁，石朝晖，蔡江帆. 高效液相色谱法同时测定畜禽组织中4种抗生素及盐酸克伦特罗残留量[J] . 中国卫生检验杂志，2010，20（7）：1642-1644.
[7] 何佳璐. 高效液相色谱法测定猪肉中的盐酸克伦特罗[J] . 海峡预防医学杂志，2006，12（2）：47-48.
[8] 钱桂平，余建中，王美全，等. 高效液相色谱法检测猪肉盐酸克伦特罗[J] . 现代预防医学，2004，31（4）：612-613.
[9] 王春荣，张济，刘岚铮，等. 高效液相色谱法测定猪肉及猪肝中的盐酸克伦特罗[J] . 中国卫生检验杂志，2005，15（1）：75-76.
[10] 陈存社，吕会田. 盐酸克伦特罗残留酶联免疫吸附（ELISA）检测方法的研究[J] . 食品与发酵工业，2006，32（7）：91-94.
[11] 邹志刚. 盐酸克伦特罗食物中毒及残留检测研究进展[J] . 预防医学论坛，2007，13（2）：145-148.
[12] AMENDOLA L，COLAMONICI C，ROSSI F，et al. Determination of clenbuterol in human urine by GC-MS-MS-MS：confirmation analysis in antidoping control[J] . J Chromatogr B Analyt Technol Biomed Life Sci，2002，773（1）：7-16.
[13] 滕南雁，刘向红，何颂华，等. 氘代同位素内标液相色谱-串联质谱法测定猪肉、猪肝中8种β受体激动剂类药物残留[J] . 中国卫生检验杂志，2011，21（7）：1617-1620.
[14] MELWANKI M B，HUANG S D，FUH M R. Three-phase solvent bar microextraction and determination of trace amounts of clenbuterol in human urine by liquid chromatography and electrospray tandem mass spectrometry[J] . Talanta，2007，72（2）：373-377.
[15] 姜艳彬，孙冠如，王海，等. 盐酸克仑特罗胶体金快速检测试纸条的研制[J] . 中国畜牧兽医，2010，37（10）：109-113.
[16] 李庆华，陈肖，彭道和，等. 盐酸克伦特罗残留检测方法研究进展[J] . 中国畜牧兽医文摘，2011，27（5）：182-183.
[17] 曹艺耀，许崇德，刘萍，等. 动物性食品中喹诺酮类抗生素残留测定[J] . 中国公共卫生，2013，29（10）：1450-1452.
[18] 邹学贤. 分析化学[M] . 北京：人民卫生出版社，2008：22-24. |
|
|
|
