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| A study on the determination of the total flavonol glycosides in health food |
| NI Zhu-nan, TAN Ying, YU Cun, ZHANG Jing, WANG Tian-jiao
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| Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou,Zhejiang,310051,China |
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Abstract Objective To develop a HPLC method for the determination of the total flavonol glycosides in health food by acid hydrolysis. Methods The sample was extracted by methanol and was hydrolyzed into 3 aglycones by acid hydrolysis. They were quercetin,kaempferide and isorhamnetin. Chromatographic separation was carried out by the method of HPLC. The content of total flavonol glycosides was calculated. Results Methanol -25% hydrochloric acid solution（4∶1）was added into selected samples,then 85 ℃water bath heating and refluxing were conducted for 30 min. Optimum chromatographic conditions were as follows. Chromatographic column：ODS C18（150 mm×4.6 mm,5μm）. Mobile phase：used methanol-0.6% phosphoric acid （47∶53）. Column temperature was set at 35 ℃. Detection wavelength was 360 nm. The linear ranges of quercetin, kaempferide and isorhamnetin were（4.766-95.52）μg/mL（r=0.999 1）,（5.052-101.0）μg/mL（r=0.999 4）and（2.027-48.66）μg/mL （r=0.999 4）respectively. The relative standard deviation of the samples was 0.57%. The recovery rate was 98.25%（RSD=3.82%）,99.81%（RSD=3.31%）and 101.8%（RSD=4.86%）. The detection limits of quercetin and kaempferide were both 0.50 μg,while Isorhamnetin was 0.80 μg,the total flavonol glycosides was 4.52 μg. The content of total flavonol glycosides detected by this method was lower than the content of the total glavonoids detected by the method provided by technical specification for inspection and evaluation of health food （2003 Edition）. Conclusion This method proved to be simple,stable and had strong repeatability and could be used for the determination of total flavonol glycosides in health food.
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Received: 14 December 2016
Revised: 18 January 2017
Published: 25 October 2017
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[1] MAHADY G B. Ginkgo biloba for the prevention and treatment of cardiovascular disease：a review of the literature[J]. J Cardiovasc Nurs,2002,16（4）：21-32.
[2] 易琼,李欣,李圆方,等. 银杏黄酮苷元对小鼠脑缺血模型和鹌鹑动脉粥样硬化模型的影响[J]. 中国新药与临床药理,2015,26（6）：804-808.
[3] 赵艳荣,张国斌,邴飞虹. 银杏黄酮对心肌缺血再灌注损伤大鼠的心肌保护作用及其作用机制研究[J]. 实用心脑肺血管病杂志,2016,24（3）：38-42.
[4] 常征,王惠兰,乔宗旭,等. 银杏黄酮、氨磷汀及亮丙瑞林对顺铂抑制卵巢恶性畸胎瘤细胞作用的影响[J]. 河北医科大学学报,2016,37（10）：1208-1211.
[5] 王远航,李微. 蒲黄提取物对内皮细胞IL-8表达的影响[J]. 中华中医药学刊,2012,30（6）：1370-1371.
[6] 杨慧玲,李军. 蒲黄总黄酮对家兔血液流变学参数和血小板聚集的影响[J]. 中国实验方剂学杂志,2012,18（17）：244-246.
[7] 范冰舵. 蒺藜地上部分化学成分研究[D]. 北京：北京中医药大学,2014.
[8] 杨莉,王国栋,韩梅. 蒺藜的活性成分积累和动态分布的研究[J]. 中草药,2013,44（9）：1186-1189.
[9] XIE J H,LIU X,SHEN M Y,et al. Purification,physicochemical characterisation and anticancer activity of a polysaccharide from Cyclocarya paliurus leaves[J]. Food Chem,2013,136（3-4）： 1453-1460.
[10] XIE J H,SHEN M Y,XIE M Y,et al. Ultrasonic-assisted extraction,antimicrobial and antioxidant activities of Cyclocarya paliurus （Batal.） Iljinskaja polysaccharides[J]. Carbohydr Polym,2012,89（1）： 177-184.
[11] 刁幼林. 紫外分光光度法测定复方银杏叶胶囊中总黄酮的含量[J]. 时珍国医国药,2001,12（12）：1073-1073.
[12] 项彦华,孙淑娜,王磊,等. 近红外光谱技术快速检测银杏叶中指标成分含量的研究[J]. 中国药物警戒,2015,12（12）：744-747.
[13] 丁晓燕,杨静红,马亚文. 高效液相色谱法测定银杏叶及相关产品中总黄酮醇苷含量[J]. 中国卫生检验杂志,2012,22（3）：464-465.
[14] 国家药典委员会. 中华人民共和国药典（一部）[M]. 北京：中国医药科技出版社,2015：316.
[15] 中华人民共和国卫生部. 保健食品检验与评价技术规范（2003年版）[S]. 北京：中国标准出版社,2003：308.
[16] 邹佳峰,吴瑶. 正交试验优选盐酸水解提取荷叶总黄酮醇苷工艺[J]. 亚太传统医药,2015,11（13）：19-21.
[17] 王兆华,纪义波,张大军. 不同提取溶剂对银杏叶药材中多种成分含量的影响[J]. 山东化工,2016,45（2）：22-23.
[18] 中华人民共和国国家质量监督检验检疫总局. 实验室质量控制规范 食品理化检测：GB/T 27404-2008[S]. 北京：中国标准出版社,2008. |
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