Abstract：Objective To understand the drug resistance of pulmonary tuberculosis（TB）patients in Jinhua and provide scientific basis for individualized treatment and the effective control of drug resistant TB. Methods The sputum specimens from TB patients reported in Jinhua TB hospital from July 1，2015 to June 30，2016 were identified. The drug sensitivity test was carried out for the specimens with positive culture and identified as Mycobacterium TB. The drug resistance rate，drug resistance type and drug resistance spectrum of the patients were analyzed. Results A total of 939 strains were obtained，and 869 strains were identified as Mycobacterium TB，accounting for 92.55%. Among the 869 TB patients，there were 168 cases of drug resistance，and the total resistance rate was 19.33%. The highest proportion of resistance spectrum was streptomycin（4.60%），and the lowest was ethambutol（0.23%）. Of 869 TB patients，802 cases were first treated and 67 cases were retreated. The total resistance rate，multiple drug resistance rate and single drug resistance rate of the retreated patients were 58.21%，29.85% and 25.37%，which were higher than 16.08%，3.24% and 10.22% of the first treated patients （P<0.05）. The drug resistance spectrum of the first treated patients was streptomycin（4.36%），isoniazide（3.24%）and ofloxacin（1.62%）. The drug resistance spectrum of the retreated patients was rifampicin + isaziazide（14.93%），isoniazide（11.94%），streptomycin（7.46%），rifampicin + isaziazide + amethybutanol + streptomycin（5.97%）and ofloxacin（4.48%）. Conclusion The total resistance rate of TB patients in Jinhua was 19.33%，and drug resistance in retreated patients was more severe than that in first treated patients.