Abstract:Objective To investigate the serotypes and drug resistance of non-typhoidal Salmonella in Zhengzhou City, so as to provide insights into prevention and control of non-typhoidal Salmonella infections. MethodsSalmonella isolates were collected from diarrheal patients in Zhengzhou municipal sentinel hospitals from 2017 to 2021. Salmonella serotypes were identified using slide agglutination test and soft agar colony formation assay, and antimicrobial susceptibility test was performed using the broth micro-dilution method. Results Five serogroups and 37 serotypes were identified among 446 non-typhoidal Salmonella isolates, with S. enteritidis (210 isolates, 47.09%) and S. typhimurium (133 isolates, 29.82%) as dominant serotypes. Non-typhoidal Salmonella showed high resistance to ampicillin (79.60%), ampicillin/sulbactam (58.74%), naphthyric acid (56.05%), tetracycline (54.26%) and doxycycline (54.04%), respectively. There were 290 multidrug-resistant Salmonella isolates (65.02%), and the multidrug resistance rates were 70.48% for S. enteritidis and 67.67% for S. typhimurium, respectively. Conclusions Multiple serotypes of non-typhoidal Salmonella were identified in Zhengzhou City from 2017 to 2021, with S. enteritidis and S. typhimurium as dominant serotypes. Widespread drug resistance and multidrug resistance was seen in non-typhoidal Salmonella.
[1] 高飞,郑浩,王洁琳,等.1 022例儿童感染沙门菌的流行特征及耐药分析[J].中华微生物医学工程杂志,2021,27(5):533-540. [2] WU H Y,XIA X D,CUI Y,et al.Prevalence of extended spectrum β-lactamase-producing Salmonella on retail chicken in six provinces and two national cities in the People's Republic of China[J].J Food Protection,2013,76(12):2040-2044. [3] 穆玉娇,张白帆,赵嘉咏,等.河南省腹泻患者沙门菌血清型及其耐药性变迁研究[J].中国卫生检验杂志,2016,26(9):1335-1338. [4] 王月,陈晓,王若南,等.浙江省临床分离沙门菌血清型、药物敏感性和毒力基因的研究[J].中华传染病杂志,2020,38(10):646-650. [5] 穆玉娇,李东晓,尤爱国,等.2015—2018年河南省儿童腹泻患者分离非伤寒沙门菌病原特征及耐药性[J].中华微生物学和免疫学杂志,2020,40(10):787-791. [6] 江南,张萍,吴建军,等.2015—2019年北京市通州区非伤寒沙门菌耐药性分析[J].中国卫生检验杂志,2021,31(18):2213-2216. [7] Clinical and Laboratory Standards Institute.M100-S26 performance standards for antimicrobial susceptibility testing[S].Wayne,PA:Clinical and Laboratory Standards Institute,2016. [8] 李春辉,吴安华.MDR、XDR、PDR多重耐药菌暂行标准定义——国际专家建议[J].中国感染控制杂志,2014,13(1):62-64. [9] 罗璇,陈中举,田磊,等.临床分离沙门菌属细菌220株的分布特征和耐药性[J].中国感染与化疗杂志,2020,20(6):659-663. [10] 周绍英,张琰,杨元斌,等.宁波市食源性腹泻病例沙门菌感染分析[J].预防医学,2020,32(9):904-906. [11] 贺连华,吴平芳,陈妙玲,等.2010—2013年深圳市食品中沙门菌血清型分型及耐药分析[J].中国卫生检验杂志,2015,25(2):289-291,294. [12] 胡玉琴,章乐怡,李毅,等.温州市沙门菌血清型、耐药性和PFGE指纹图谱研究[J].预防医学,2019,31(6):640-642. [13] 陈盛杰,曾莹春,霍细香,等.湖北省不同来源沙门菌耐药及耐药谱研究[J].现代预防医学,2017,44(6):1011-1014. [14] 胡付品,郭燕,朱德妹,等.2021年CHINET中国细菌耐药监测[J].中国感染与化疗杂志,2022,22(5):521-530. [15] 杨翊,贾华云,任国锋,等.2013—2017年湖南省沙门菌耐药状况及其分布[J].中国抗生素杂志,2020,45(6):621-626.