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| Anti-apoptotic effect of s-nitrosoglutathione on platelet in vitro |
| HUA Lin, YU Lu, HE Yun-lei, DENG Gang
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| The Ningbo Central Blood Station,Ningbo,Zhejiang 315000,China |
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Abstract Objective To investigate the effects of nitric oxide（NO）donor s-nitrosoglutathione（GSNO）on apoptosis of apheresis platelets cells during storage. Methods Six platelets were collected and divided into two bags aseptically. One bag for experimental group and the other for control group into which a GSNO solution with a certain concentration was added into fresh apheresis platelets. Scanning electron microscope,flow cytometry and Western Blot were used to detect platelet morphology,apoptosis markers and the expression of apoptosis-related protein,respectively. All the parameters were dynamically monitored at the 1st,3rd,5th,and 7th day. Results There was a difference of NO concentration between GSNO group and control group（P<0.05）,the NO level in GSNO group were remarkably higher than those in the control group during the whole storage stage. Platelet scanning electron microscopy showed that the morphology of platelets in GSNO group was superior to that of the control group. There were differences in lactate acid content（P<0.05）and glucose（P<0.05）in GSNO group and the control group,and GSNO group was significantly lower than control group at some time point. Flow analysis showed the positive ratio of phosphatidyl serine（PS）exposed on the cell surfaces,and GSNO group was lower than control group （P<0.05）. There was no statistical difference between the relative expression levels of apoptosis-related protein （P>0.05）. Conclusion The addition of NO donor GSNO in platelet preservation can inhibit platelet cell apoptosis and reduce platelet storage lesion in vitro during the platelet storage period.
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Published: 22 May 2018
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