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| A study on the influence of montelukast on chromogranin A protein and mRNA expressions at lung tissue of asthmatic rat |
| LYU Qing-qing,JIN Xiao-hong,LI Shao-bo,TONG Xia-sheng,ZHANG Wei-xi,LUO Dong-jiao
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| Department of Pediatrics, Affiliated Taizhou Hospital of Wenzhou Medical College,Lin Hai,Zhejiang, 317000,China |
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Abstract Objective To investigate the potential roles of chromogranin A in pathogenesis of asthmatic inflammation, and to assess the regulation of montelukast on chromogranin A expression.Methods The rat asthma model was established with ovalbumin, and they were allocated to three groups, named asthma group, control group and montelukast group. The expressions of chromogranin A protein and mRNA at lung tissue were detected by immunohistochemisty or real-time PCR methods, and positive expression intensity of chromogranin A protein was assayed by optical density. The correlation between chromogranin A protein and mRNA was also analyzed.Results The expression levels of chromogranin A protein in asthma group(0.34±0.05 optical density) was significantly higher than that in control group (0.21±0.06 optical density)(P＜0.01). The expression levels of chromogranin A mRNA in asthma group (4.02±0.95 relative quantity value)was significantly higher than that in control group(P＜0.01).The expression levels of chromogranin A protein in montelukast group(0.28±0.04 optical density)was dramatically lower than that in asthma group(0.34±0.05 optical density)(P＜0.05), while there were no statistical significance of chromogranin A mRNA(3.67±0.78 relative quantity value) between those two groups(P＞0.05). But levels of mRNA was positively correlated with protein of chromogranin A (r=0.635,P＜0.01).Conclusion Expressions of chromogranin A protein and mRNA at lung tissue were increased in asthmatic rats, and the results demonstrated that chromogranin A perhaps participated in the pathogenesis of asthma inflammation, but this function of chromogranin A protein could be down regulated by montelukast.
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Published: 26 December 2017
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