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| A study on the effects of dendrobium officinale leaves on sperm quality and testicular tissue morpholohy in parent and offspring rats |
| FU Jian-yun, SONG Yan-hua, HE Jia-wei, ZHU Zhou-jing, SONG Song, XIA Yong
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| Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou,Zhejiang,310051,China |
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Abstract Objective To investigate the effects of dendrobium officinale leaves on sperm quality and testicular tissue morphology in parent（P） and offspring（F1、F2） rats. Method s P、F1、F2 rats were fed with dendrobium officinale leaves at the dose of 0,2.0,4.0,6.4g/kg·bw for two generation reproduction,and the testicular and epididymal viscera coefficients,the quantity and quality of sperm were examined and the histopathological assessment was carried out. Results There were no statistical differences in the testicular and epididymal viscera coefficients,sperm quantity,sperm motility rate and sperm malformation rate compared with the control group in P,F1,F2 rats（P>0.05）. There were no differences between 0 and 6.4 g/kg·bw groups on the testicular and epididymal tissue morphology in P,F1,F2 rats. Conclusion Dendrobium officinale leaves didn’t show obvious adverse effects on sperm quality and testicular tissue morphology in P,F1,F2 rats.
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Received: 14 September 2016
Revised: 09 October 2016
Published: 17 October 2017
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