|
|
|
| A study on the effects of Dendrobium candidum leaves on the two generation reproduction female rats' immune function |
| SONG Yan-hua, CAI De-lei, FU Jian-yun, XIA Yong, XU Cai-ju, LU Wei
|
| The Center for Disease Control and prevention of Zhejiang Province, Hangzhou, Zhejiang, 310051, China |
|
|
|
|
Abstract Objective To investigate the effects of Dendrobium candidum leaves on immune function of female rats after two generation reproduction.Methods Different doses of Dendrobium candidum leaves (2.0 g/kg-6.4 g/kg B.W) were fed to the rats for two generation reproduction. The thymus/body ratio,the spleen/body ratio,the T lymphocyte proliferation of rat spleen induced by conA, delayed type hypersensitivity, serum hemolysin antibody, the antibody-producing cells, the phagocytotic ability of peritoneal macrophages for chicken erythrocytes, the carbon clearance indexes and natural killer(NK)activity were detected.Results Compared with the control group, Dendrobium candidum leaves could increase the ability of T lymphocyte proliferation of mouse spleen induced by conA. NK cell function, the delayed type hypersensitivity in the rats of high dose were higher than the control group(P<0.05).There were no significant differences among groups on the number of hemocytolysis plaque,macrophage activity, the level of hemolytic antibody in serum, carbon clearance, the thymus/body ratio, the spleen/body ratio, and the weight gain of the rats (P>0.05).Conclusion Under this experimental condition, Dendrobium candidum leaves could enhance the T lymphocyte proliferation, the delayed type hypersensitivity and NK cell function of female rats after two generation reproduction.
|
|
Received: 19 June 2015
Published: 20 December 2017
|
|
|
|
|
|
[1]高正华,杨兵勋,陈立钻.铁皮石斛的研究进展.中国现代应用药学,2008,25(8):692-695.
[2]李桂峰,许进进,许继勇,等.铁皮石斛研究综述.中草药,2010,33(1):150-153.
[3]吴建,王建芳,方玲,等.国内铁皮石斛研究概况.中国药学杂志,2013,48(19):1610-1613.
[4]L Xiang,C W Stephen Sze,T B Ng,et al.Pdysaccharides of Dendrobium officinale inhibit TNF-α-induced apoptosis in A-253 cell line. Inflamm Res,2013,62(3):313-324.
[5]黄月纯,杨丽娥,刘宏源,等.鲜铁皮石斛茎、叶高效液相色谱特征图谱比较.广州中医药大学学报,2012,29(5):561-565.
[6]李光,宋美芳,李宜航,等. 不同种类石斛多糖成分对小鼠脾脏免疫功能的影响.中国临床药理学与治疗学, 2012,17(10): 1108-1111.
[7]刘亚娟,王诗豪,张铭,等. 铁皮石斛多糖抗癌及免疫活性研究. 广州化工, 2014,42(10): 58-60,65.
[8]中华人民共和国卫生部.保健食品检验与评价技术规范.2003:22-34.
[9]唐汉庆,韦神,卢兰,等.铁皮石斛对小鼠抗氧化能力影响的实验研究.中国卫生检验杂志,2014, 24(21):3082-3084.
[10]吴建,王建方,方玲,等.国内铁皮石斛研究概况.中国药学杂志, 2013,48(19):1610-1613.
[11]陈晓梅,王春兰,杨峻山,等.铁皮石斛化学成分及其分析的研究进展.中国药学杂志,2013,48(19):1634-1640.
[12]陈素红,颜美秋,吕桂源,等.铁皮石斛保健食品开发现状与进展.中国药学杂志,2013,48(19):1625-1628.
[13]周桂芬,吕圭源.基于高效液相色谱-二极管阵列光谱检测-电喷雾离子化质谱联用鉴定铁皮石斛叶中8种黄酮碳苷化合物及裂解规律研究.中国药学杂志,2012,47(1):13-19.
[14]周桂芬,吕圭源.铁皮石斛不同部位黄酮碳苷类成分及清除DPPH自由基能力比较研究 .中国中药杂志,2012,37(11):1536-1540. |
|
|
|
