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| A study on the oxidative stress induced by ambient fine particulate matter (PM2.5) and water-soluble fraction on HBE cells |
| ZHANG Shi-xin, WU Li-zhi, CHEN Qing, ZHENG Yun-yan, CAI De-lei
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| The Center for Disease Control and Prevention of Zhejiang Province, Hangzhou, Zhejiang, 310051, China |
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Abstract ObjectiveTo investigate the cytotoxicity and oxidative stress of ambient fine particulate matter (PM2.5) and water-soluble fraction of PM2.5 on human bronchial epithelial cells (HBE ). MethodsPM2.5 samples were collected in the urban area of Hangzhou. Then the water-soluble fraction was extracted from PM2.5. After HBE cells were exposed to PM2.5 and its water-soluble fraction at the doses of 0, 100, 250, 500, 1 000, 1 500 and 2 000 μg/mL for 24 h, CCK-8 (cell counting kit-8 )assay was conducted to examine the cytotoxicity of the PM2.5 and its water-soluble fraction. The oxidative damage induced by PM2.5 and its water-soluble fraction on HBE cells was then evaluated with lipid peroxidation, the superoxide dismutase (SOD) activity, and the levels of glutathione peroxidase (GSH-Px). ResultsThe PM2.5 and its water-soluble fraction reduced the viability of HBE cells in a dose-dependent manner. When the PM concentrations were 200, 400 and 800 μg/mL, the SOD activity of the HBE cells decreased significantly, as compared with the control group (P< 0.05). Also, the malondialdehyde (MDA) levels of the HBE cells significantly increased at the doses of 200, 400 and 800 μg/mL (P< 0.05). However, there were no significant differences of GSH-Px activity among the groups. ConclusionThe PM2.5 and its water-soluble fraction could induce cytotoxic and oxidative damage effects on the HBE cells.
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Received: 09 July 2015
Revised: 14 August 2015
Published: 07 December 2017
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