|
|
|
| Combined effects of triclosan and PCB153 on the activity of superoxide dismutase and the content of malondialdehyde in zebrafish liver |
| ZHOU Cheng, WU Nan-xiang, FAN Hong-liang, YANG Ye, GAO Ming, CHEN Die, SHEN Hong
|
| Institute of Hygiene,Zhejiang Academy of Medical Sciences,Hangzhou,Zhejiang 310013,China |
|
|
|
|
Abstract Objective To investigate the combined effects of triclosan（TCS）and PCB153 on the activity of superoxide dismutase（SOD）and the concentration of malondialdehyde（MDA）in zebrafish liver.Methods Adult zebrafish were exposed to a series of concentrations of TCS,and the mortality in each group was observed and recorded during the acute toxicity test process. The concentrations in subsequent combined exposure experiments were arranged on the basis of the 96 h-LC50. The factorial design was used to determine the concentrations of combined exposure groups between TCS（0,0.125,0.5 μmol/L）and PCB153（0,0.05,0.2 μmol/L）. After 5,10 and 14 days of exposure,the zebrafish livers were dissected and frozen in each group. The potential interactions of these two compounds were analyzed according to the results of the SOD and MDA.Results The 96 h-LC50 of TCS exposed to adult zebrafish was 2.64 μmol/L（95%CI：2.37-2.89 μmol/L）. After 5 days of exposure,combined exposure to 0.5 μmol/L TCS+0.2 μmol/L PCB153 caused lower liver SOD activities than single exposure groups and the control group（P<0.05）. After 10 days of exposure,combined exposure to 0.125 μmol/L TCS+0.05 μmol/L PCB153,0.5 μmol/L TCS+0.05 μmol/L PCB153 caused lower liver SOD activities than single exposure groups and the control group（P<0.05）. After 14 days of exposure,combined exposure to 0.5 μmol/L TCS+0.05 μmol/L PCB153,0.5 μmol/L TCS+0.2 μmol/L PCB153 caused higher liver SOD activities than single exposure groups and the control group（P<0.05）. There was an interactive effect between TCS and PCB153 on the liver SOD activity in zebrafish（P<0.05）. There was no significant effect of MDA content in each group.Conclusion Combined exposure to TCS and PCB153 could enhance （inhibit first） the liver SOD activities in zebrafish,and the interaction was synergistic.
|
|
Received: 30 November 2018
Revised: 15 January 2019
Published: 02 April 2019
|
|
|
|
|
|
[1] HALDEN R U.On the need and speed of regulating triclosan and triclocarban in the United States[J]. Environ Sci Technol,2014, 48(7):3603-3611.
[2] BEDOUX G,ROIG B,THOMAS O,et al.Occurrence and toxicity of antimicrobial triclosan and by-products in the environment[J]. Environ Sci Pollut Res Int,2012,19(4):1044-1065.
[3] ZHU W,ZHANG H,TONG C,et al.Environmental exposure to triclosan and semen quality[J]. Int J Environ Res Public Health,2016,13(2):224-235.
[4] YIN J,WEI L,SHI Y,et al.Chinese population exposure to triclosan and triclocarban as measured via human urine and nails[J]. Environ Geochem Health,2016,38(5):1125-1135.
[5] 金辰烨,陈依明,张佩祺,等. 人群三氯生暴露水平研究进展[J]. 中华预防医学杂志,2016,50(3):285-288.
[6] HALDEN R U,LINDEMAN A E,AIELLO A E,et al.The florence statement on triclosan and triclocarban[J]. Environ Health Perspect,2017,125(6):e064501.
[7] ZANI C,TONINELLI G,FILISETTI B,et al.Polychlorinated biphenyls and cancer:an epidemiological assessment[J]. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev,2013,31(2):99-144.
[8] FAROON O,RUIZ P.Polychlorinated biphenyls:new evidence from the last decade[J]. Toxicol Ind Health,2016,32(11):1825-1847.
[9] MING-CH'ENG ADAMS C I,BAKER J E,KJELLERUP B V. Toxicological effects of polychlorinated biphenyls(PCBs)on freshwater turtles in the United States[J]. Chemosphere,2016,154:148-154.
[10] SPANO M,TOFT G,HAGMAR L,et al.Exposure to PCB and p,p'-DDE in European and Inuit populations:impact on human sperm chromatin integrity[J]. Hum Reprod,2005,20(12):3488-3499.
[11] 孙冰,许慧慧,黄超,等. 环境毒理学研究的重要模式生物——斑马鱼[J]. 环境与职业医学,2016,33(6):610-614.
[12] 夏勇,徐彩菊,傅剑云,等. 五氯酚钠诱导斑马鱼体细胞p53基因突变的克隆测序研究[J]. 预防医学,2017,29(4):325-329.
[13] DE S,GHOSH S,CHATTERJEE R,et al.PCB congener specific oxidative stress response by microarray analysis using human liver cell line[J]. Environ Int,2010,36(8):907-917.
[14] 刘贝贝,陈剑锋,张勇. 多氯联苯的肝毒性研究进展[J]. 环境与健康杂志,2009,26(1):90-92.
[15] WANG F,XU R,ZHENG F,et al.Effects of triclosan on acute toxicity,genetic toxicity and oxidative stress in goldfish(Carassius auratus)[J]. Exp Anim,2018,67(2):219-227.
[16] Organization for Economic Co-operation and Development. Short guidance on the threshold approach for acute fish toxicity[EB/OL].(2010-05-31)[2018-09-28]. http://www.oecd.org/officialdocuments/publicdisplaydocumentpdf/?cote=ENV/JM/MON(2010)17& docLanguage=En.
[17] 中华人民共和国国家环境保护总局. 水质物质对淡水鱼(斑马鱼)急性毒性测定方法:GB/T 13267—1991[S]. 北京:中国标准出版社,1992.
[18] 徐娟,吴南翔,陈琼姜,等. Aroclor1254对斑马鱼胚胎发育、肝脏组织结构及其卵黄蛋白原基因的影响[J]. 环境与职业医学,2011,28(9):577-581.
[19] LOU J,SONG P,WU N,et al.Chemokine(C-C motif)ligand 22 is down-regulated in a human B lymphoblastoid cell line by PCB153 and in residents from PCBs-contaminated areas[J]. Mutat Res,2013,752(1-2):21-27.
[20] US Environmental Protection Agency. Supplementary guidance for conducting health risk assessment of chemical mixtures[EB/OL].[2018-09-28]. http://www.epa.gov/raf/publications/pdfs.
[21] ABREU I A,CABELLI D E.Superoxide dismutases-a review of the metal-associated mechanistic variations[J]. Biochim Biophys Acta,2010,1804(2):263-274.
[22] LYKKESFELDT J.Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking[J]. Clin Chim Acta,2007, 380(1-2):50-58.
[23] SIES H.Oxidative stress:a concept in redox biology and medicine[J]. Redox Biol,2015,4:180-183. |
|
|
|
