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| A toxicological evaluation on the safety of the Eucommia folium |
| ZHU Zhou-liang, YAN Jun, ZHENG Yun-yan, XIA Yong, FU Jian-yun, MENG Zhen, SONG Song
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| The Center for Disease Control and Prevention of Zhejiang Province,Hangzhou,Zhejiang,310051,China |
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Abstract Objective To evaluate the safety of the Eucommia folium and to provide the basis of the rational use of Eucommia folium resource. Methods According to < Technical Standards for Testing & Assessment of Health Food>,version 2003,acute safety test/micronucleus test of born marrow in mice/sperm shape abnormality test in mice/Ames test and thirty-day feeding test were all performed after water extract concentrations of Eucommia folium treatment（1 mL concentrations equal to 1.5 g Eucommia folium）. Results On the acute toxicity test,the MTD of rats and mice were both 40.0 mL/kgbw. The results of genetic toxicity test were all negative in the dosage of 10 g/kg bw（according to the dosage of Eucommia folium）.The results of thirty-day feeding test indicated that there have no significant differences in body weight,blood cytology indexes,blood biochemical indexes,organ/body ratio and pathematology examination in the rats in all the groups （0.83,1.67,3.30 mL/kg） as compared to the control group（P>0.05）. Conclusion Under this experimental condition,no obvious abnormity and toxic reaction were observed.
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Received: 26 April 2016
Revised: 08 September 2016
Published: 27 October 2017
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