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A study on the mRNA expression of cell cycle related genes in population occupationally exposed to hexavalent chromium |
GUO Xin-nian, TONG Yan, JIA Jun-lin, WANG Hai-ming, FENG Ling-fang, XIA Hai-ling, JIANG Zhao-qiang, LOU Jian-lin
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Zhejiang Academy of Medical Sciences,Hangzhou,Zhejiang,310000,China |
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Abstract Objective The aim of our study was to investigate the influence of hexavalent chromium exposure on mRNA expression of cell cycle related genes in electroplating workers,and to provide population data for investigating the toxic mechanisms of hexavalent chromium. Methods A total of 155 cases of workers occupationally exposed to hexavalent chromium were selected,including 89 males and 66 females,and the average age of workers was 39.65±8.856 years old. Questionnaire was used to collect essential information of workers. Peripheral blood was collected from electroplating workers. The inductively couple plasma mass spectrometry(ICP-MAS)was used to measure total blood chromium content. The workers were divided into four groups according to the blood chromium content. After extracting total RNA from whole blood and reverse transcription,the mRNA expression levels of p16 and CDK6 genes were detected by real-time quantitative PCR. Meanwhile,the levels of blood chromium (BCr)and the mRNA expression of p16 and CDK6 genes were compared among four groups. The impact of BCr,smoking habits,drinking habits,gender on the mRNA expression of CDK6 gene was analyzed. Results The levels of BCr in group 1 to 4 were 0.04ppb,0.47±0.29 ppb,2.76±1.16 ppb,9.36±4.38 ppb,respectively,and the difference between every two groups was significant (P<0.05). The median of p16 gene expression in four groups was 4.22,7.19,7.47,and 14.60,respectively,and the difference between every two groups was not significant (P>0.05). The mRNA expression levels of CDK6 gene in groups 2 to 4 were 15.05,8.03 and 24.81,respectively,which were significantly higher than that in group 1 (P<0.05). The results of logistic regression showed that the level of Bcr was the main influence factor,while smoking habits,drinking habits and gender had no obvious impact on the mRNA expression of CDK6 gene. Conclusions Long-term exposure of hexavalent chromium led to higher mRNA expression of CDK6 gene,and it may serve as a biomarker for workers occupationally exposed to hexavalent chromium.
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Received: 07 December 2016
Revised: 07 April 2017
Published: 15 November 2017
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[1] MULWARE S J. Trace elements and carcinogenicity: a subject in review[J]. 3 Biotech,2013,3(2):85-96.. [2] GIBB H J,LEES P S,WANG J,et al. Extended followup of a cohort of chromium production workers[J]. American Journal of Industrial Medicine,2015,58(8):905-913. [3] ASHOUSH S,ABOU-GAMRAH A,BAYOUMY H,et al. Chromium picolinate reduces insulin resistance in polycystic ovary syndrome:randomized controlled trial[J]. J Obstet Gynaecol Res,2016,42(3):279-285.. [4] XIE H,HOLMES A L,WISE S S,et al. Human skin cells are more sensitive than human lung cells to the cytotoxic and cell cycle arresting impacts of particulate and soluble hexavalent chromium[J]. Biol Trace Elem Res,2015,166(1):49-56. [5] BURHANS W C,HEINTZ N H. The cell cycle is a redox cycle:linking phase-specific targets to cell fate[J]. Free Radic Biol Med,2009,47(9):1282-1293. [6] CIESIELSKA U,ZATONSKI T,NOWINSKA K,et al. Expression of cell cycle-related proteins p16,p27 and Ki-67 proliferating marker in laryngeal squamous cell carcinomas and in laryngeal papillomas[J]. Anticancer Res,2017,37(5):2407-2415. [7] LOU J,WANG Y,YAO C,et al. Role of DNA methylation in cell cycle arrest induced by Cr (VI) in two cell lines[J]. PLoS ONE,2013,8(8):e71031. [8] BONNEFOY C,MENUDIER A,MOESCH C,et al. Determination of chromium in whole blood by DRC-ICP-MS:spectral and non-spectral interferences[J]. Anal Bioanal Chem,2005, 383(2):167-173. [9] 王禹. 六价铬暴露与p16基因启动子区DNA甲基化之间关系的研究[D]. 杭州:浙江省医学科学院,2014. [10] 何廷德,刘武林. 妊娠期血脂生理性变化趋势及正常参考值研究[J]. 检验医学与临床,2015,12(9):1271-1272, 1275. [11] GATTO N M,KELSH M A,MAI D H,et al. Occupational exposure to hexavalent chromium and cancers of the gastrointestinal tract:a meta-analysis[J]. Cancer Epidemiol,2010,34(4):388-399. [12] HEDBERG Y S,LID #x000c9;N C,LINDBERG M. Chromium dermatitis in a metal worker due to leather gloves and alkaline coolant[J]. Acta Derm Venereol,2016,96(1):104-105. [13] JAGANNATI M,RAMYA I,SATHYENDRA S. Occupation- related chromium toxicity a rare cause of renal failure and rhabdomyolysis[J]. Indian J Occup Environ Med,2016,20(3):150-152. [14] SHELNUTT S R,GOAD P,BELSITO D V. Dermatological toxicity of hexavalent chromium[J]. Crit Rev Toxicol,2007,37(5):375-387. [15] STANLEY J A,LEE J,NITHY T K,et al. Chromium-VI arrests cell cycle and decreases granulosa cell proliferation by down-regulating cyclin-dependent kinases (CDK)and cyclins and up-regulating CDK-inhibitors[J]. Reprod Toxicol,2011, 32(1):112-123. [16] RODRIGUEZ E,AZEVEDO R,FERNANDES P,et al. Cr(VI)induces DNA damage,cell cycle arrest and polyploidization:a flow cytometric and comet assay study in Pisum sativum[J]. Chem Res Toxicol,2011,24(7):1040-1047. [17] CHIU A,SHI X L,LEE W K,et al. Review of chromium (VI)apoptosis,cell-cycle-arrest,and carcinogenesis[J]. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev,2010,28(3):188-230. [18] WAKEMAN T P,KIM W J,CALLENS S,et al. The ATM- SMC1 pathway is essential for activation of the chromium[VI]-induced S-phase checkpoint[J]. Mutat Res,2004,554(1-2):241-251. [19] KONDO K,TAKAHASHI Y,HIROSE Y,et al. The reduced expression and aberrant methylation of p16(INK4a)in chromate workers with lung cancer[J]. Lung Cancer,2006,53(3):295-302. |
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