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| Effect of light at night on liver metabolism in miceby untargeted metabolomics |
| ZHANG Rong*, LIU Chunhua, HU Shuang, LI Pengxiang, WEI Shougang
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| *School of Public Health, Capital Medical University, Beijing 100069, China |
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Abstract Objective To observe the changes of liver metabolism in mice exposed to artificial light at night. Methods Healthy male C57BL/6J mice were randomly divided into the light at night group and the control group, with 8 mice in each group. The daily light/dark cycle was 12/12 hours in the control group, and 24/0 hours in the light at night group for 10 consecutive days. The hepatic metabolite profiles of the two groups of mice were detected by high performance liquid chromatography tandem mass spectrometry. The modelling was assessed by combining principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis ( OPLS-DA ) , The changes of metabolites in the two groups were compared through KEGG database. Results Compared with the control group, 9 different metabolites were detected in the light at night group, among which the down-regulated metabolites were glycine-betaine, glutathione, tyrosine, betaine, lysine, hypoxanthine, histidine and methionine, and the up-regulated ones were mannose-6-phosphate. The weight analysis of the metabolic pathways showed that the major influences on liver of light at night group were phenylalanine-tyrosine-tryptophan metabolism, tyrosine metabolism, fructose and mannose metabolism, cysteine and methionine metabolism and histidine metabolism. Conclusion The metabolism of various amino acids and sugars in light at night mice is disturbed,and the key differential metabolites are tyrosine, methionine, histidine and mannose-6-phosphate.
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Received: 10 October 2020
Revised: 25 November 2020
Published: 01 February 2021
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[1] BAUER M,GLENN T,MONTEITH S,et al.The potential influence of LED lighting on mental illness[J].World J Biol Psychiatry,2018,19(1):59-73.
[2] ASKENAZI L,HAIM A.Effect of light at night on oxidative stress markers in golden spiny mice(Acomys russatus)liver[J].Comp Biochem Physiol A Mol Integr Physiol,2013,165(3):353-357.
[3] RUSART K L G,NELSON R J.Light at night as an environmental endocrine disruptor[J].Physiol Behav,2018,190:82-89.
[4] CHO Y M,RYU S H,LEE B R,et al.Effects of artificial light at night on human health:a literature review of observational and experimental studies applied to exposure assessment[J].Chronobiol Int,2015,32(9):1294-1310.
[5] MARK Y M,WHITE A J,JACKSON C L,et al.Association of exposure to artificial light at night while sleeping with risk of obesity in women[J].JAMA Intern Med,2019,179(8):1061-1071.
[6] FONKEN,LAURA Ket al.Dim light at night disrupts molecular circadian rhythms and increases body weight[J].J Biol Rhythms,2013,28(4):262-271.
[7] OPPERHUIZEN A L,STENVERS D J,JANSEN R D,et al.Light at night acutely impairs glucose tolerance in a time-,intensity-and wavelength-dependent manner in rats[J].Diabetologia,2017,60(7):1333-1343.
[8] CAILOTTO C,LEI J,VAN DER VLIET J,et al.Effects of nocturnal light on(clock)gene expression in peripheral organs:a role for the autonomic innervation of the liver[J].PLoS One,2009,4(5):e5650.
[9] YAN L,LONSTEIN J S,NUNEZ A A.Light as a modulator of emotion and cognition:lessons learned from studying a diurnal rodent[J].Horm Behav,2019,111:78-86.
[10] AN K,ZHAO H,MIAO Y,et al.A circadian rhythm-gated subcortical pathway for nighttime-light-induced depressive-like behaviors in mice[J].Nat Neurosci,2020,23(7):869-880.
[11] OBAYASHI K,SAEKI K,KURUMATANI N.Bedroom light exposure at night and the incidence of depressive symptoms:a longitudinal study of the HEIJO-KYO Cohort[J].Am J Epidemiol,2018,187(3):427-434.
[12] GELENBERG A J,GIBSON C J.Tyrosine for the treatment of depression[J].Nutr Health,1984,3(3):163-173.
[13] STRASER B,SPERNER-UNTERWEGER B,FUCHS D,et al.Mechanisms of inflammation-associated depression:immune influences on tryptophan and phenylalanine metabolisms[J].Curr Top Behav Neurosci,2017,31:95-115.
[14] MARKOVA M,PIVOVAROVA O,HORNEMANN S,et al.Isocaloric diets high in animal or plant protein reduce liver fat and inflammation in individuals with type 2 diabetes[J].Gastroenterology,2017,152(3):571-585.
[15] NELSON R J,CHBEIR S.Dark matters:effects of light at night on metabolism[J].Proc Nutr Soc,2018,77(3):223-229.
[16] 张频,黄砚青,王雪芬,等.腺苷蛋氨酸对酒精性肝损伤大鼠甲硫氨酸代谢和脂质过氧化的影响[J].临床肝胆病杂志,2007,23(1):31-33.
[17] FENG R N,NIU Y C,SUN X W,et al.Histidine supplementation improves insulin resistance through suppressed inflammation in obese women with the metabolic syndrome:a randomised controlled trial[J].Diabetologia,2013,56(5):985-994.
[18] GONZALEZ P S,O＇PREY J,CARDACI S,et al.Mannose impairs tumour growth and enhances chemotherapy[J].Nature,2018,563(7733):719-723.
[19] FIGUEIRO M G,RADETSKY L,PLITNICK B,et al.Glucose tolerance in mice exposed to light-dark stimulus patterns mirroring dayshift and rotating shift schedules[J/OL].Sci Rep,2017,7[2020-11-25].https://pubmed.ncbi.nlm.nih.gov/28079162. DOI: 10.1038/srep40661.
[20] VERSTEEG R I,STENVERS D J,KALSBEEK A,et al.Nutrition in the spotlight:metabolic effects of environmental light[J].Proc Nutr Soc,2016,75(4):451-463. |
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