|
|
|
| Determination of perfluorocarboxylic acids in fish by ultra performance liquid chromatography tandem mass spectrometry |
| LIU Shaoying, JIN Quan, REN Ren, HUANG Xihui
|
| Hangzhou Center for Disease Control and Prevention, Hangzhou, Zhejiang 310021, China |
|
|
|
|
Abstract Objective To develop the ultra performance liquid chromatography tandem mass spectrometry ( UPLC-MS/MS ) for the determination of perfluorocarboxylic acids ( PFCAs ) in fish. Methods The fish samples were extracted with tert-butyl methyl ether and purified by WAX columns. The WAX cartridges were rinsed with methanol and 25 mmol/L ammonium acetate, and the target compound residues were eluted with 0.5% ammonia methanol and then redissolved with 50% methanol aqueous solution after nitrogen blowing to nearly dry. Nine kinds of PFCAs were simultaneously quantified by UPLC-MS/MS with 1 mmol/L ammonium acetate-methanol solution as the mobile phase. Results The extraction was separated well in UPLC BEH C18 column. There were good linear correlations of nine kinds of PFCAs in the range of 1.0-200.0 ng/mL, with the coefficients all more than 0.99. The limits of detection and quantification were 0.06-0.19 μg/kg and 0.19-0.62 μg/kg, respectively. The recovery rates were 70.08%-117.24% at different spiked levels ( 5.0, 25.0, 50.0 μg/kg ), and the relative standard deviations were 2.31%-19.68%. Conclusion Through optimizing the pretreatment conditions, the mobile phase of liquid chromatography and the detection conditions of mass spectrometry, the UPLC-MS/MS could meet the monitoring requirements of PFCAs in fish.
|
|
Received: 01 September 2020
Revised: 21 October 2020
Published: 10 December 2020
|
|
|
|
|
|
[1] FAIR P A,WOLF B,WHITE N D,et al.Perfluoroalkyl substances ( PFASs ) in edible fish species from Charleston Harbor and tributaries,South Carolina, United States: exposure and risk assessment[J].Environ Res,2019,171:266-277.
[2] 李磊,周贻兵,刘利亚,等.QuEChERS净化-超高效液相色谱-串联质谱法快速测定母乳中9种全氟化合物[J].现代预防医学,2018,45(11):2028-2033.
[3] HURLEY S,GOLDBERG D,WANG M,et al.Breast cancer risk and serum levels of per- and poly-fluoroalkyl substances:a case-control study nested in the California Teachers Stud[J].Environ Health,2018,17:266-277.
[4] KONG B,WANG X,HE B,et al.8∶2 fluorotelomer alcohol inhibited proliferation and disturbed the expression of pro-inflammatory cytokines and antigen-presenting genes in murine macrophages[J].Chermosphere,2019,219:1052-1060.
[5] NIU J,LIANG H,TIAN Y,et al.Prenatal plasma concentrations of perfluoroalkyl and polyfluoroalkyl substances and neuropsychological development in children at four years of age[J]. Environ Health,2019,18(1):53-66.
[6] LIEW Z,GOUDARZI H,OULHOTE Y.Developmental exposures to perfluoroalkyl substances (PFASs):an update of associated health outcomes[J].Curr Environ Health Rep,2018,5(1):1-19.
[7] 常思思,姚谦,田英,等.全氟化合物暴露对婴幼儿甲状腺激素水平影响的研究进展[J].上海预防医学,2019,31(9):773-777.
[8] DOMINGO J L.Health risks of dietary exposure to perfluorinated compounds[J].Environ Int,2012,40(1):187-195.
[9] 谢琳娜,张海婧,侯沙沙,等.固相萃取-超高效液相色谱-串联质谱法分析土壤中15种全氟化合物[J].分析化学,2019,47(12):1967-1972.
[10] 孙敏,鲍俊信,徐蓓,等.饮用水厂排泥水及污泥中全氟化合物分布特征[J].浙江大学学报(工学版),2019,53(9):1835-1842.
[11] 张子豪,肖前,钟怀宁,等. 液-液萃取/气相色谱-串联质谱测定纸制食品接触材料中9种挥发性全氟化合物前体物的迁移量[J]. 分析测试学报,2018,37(9):1002-1007.
[12] MOTAS GUZMAN M,CLEMENTINI C,DOLORES PEREZ-CARCELES M,et al.Perfluorinated carboxylic acids in human breast milk from Spain and estimation of infant's daily intake[J].Sci Total Environ,2016,544:595-600.
[13] HAUG L S,SALIHOVIC S,JOGSTEN I E,et al.Levels in food and beverages and daily intake of perfluorinated compounds in Norway[J].Chemosphere,2010,80(10):1137-1143.
[14] ZHOU Y,LIAN Y,SUN X,et al.Determination of 20 perfluoroalkyl substances in greenhouse vegetables with a modified one-step pretreatment approach coupled with ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS-MS)[J]. Chemosphere, 2019,227:470-479.
[15] MULABAGAL V,LIU L,QI J,et al.A rapid UHPLC-MS/MS method for simultaneous quantitation of 23 perfluoroalkyl substances (PFAS) in estuarine water[J].Talanta,2018,190:95-102.
[16] 叶洪丽,余玮玥,史永富,等.东海沿岸省市鱼类水产品中全氟烷基化合物含量调查研究[J].中国渔业质量与标准,2019,9(4):13-21.
[17] MENG J,LIU S,ZHOU Y,et al.Are perfluoroalkyl substances in water and fish from drinking water source the major pathways towards human health risk?[J].Ecotoxicol Environ Saf,2019,181:194-201.
[18] CAO X,WANG C,LU Y,et al.Occurrence, sources and health risk of polyfluoroalkyl substances (PFASs) in soil, water and sediment from a drinking water source area[J]. Ecotoxicology Environ Saf,2019,174:208-217.
[19] GAO Y,LI X M,LI X Q,et al.Simultaneous determination of 21 trace perfluoroalkyl substances in fish by isotope dilution ultrahigh performance liquid chromatography tandem mass spectrometry[J]. J Chromatogr B Analyt Technol Biomed Life Sci,2018,1084:45-52. |
|
|
|
