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| Effects of hexavalent chromium on cell cycle and expression of related genes in human B lymphoblastoid cells |
| JIA Jun-lin，TONG Yan，GUO Xin-nian，FANG Yuan，WANG Jing，LU Wei，FENG Ling-fang，XIA Hai-ling，JIANG Zhao-qiang，YU Min，JU Li，ZHU Li-jin，YING Shi-bo，LOU Jian-lin
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| Institute for Occupational Hazard Prevention，Zhejiang Academy of Medical Sciences，Hangzhou，Zhejiang 310000，China |
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Abstract Objective To investigate the influence of hexavalent chromium exposure on cell cycle and the expression levels of cell cycle related genes in human B lymphoblastoid cells. Methods Human B lymphoblastoid cells were treated with 5 μM potassium dichromate for 24 hours and then incubated in fresh medium. The cells in the control group were treated with phosphate buffer saline. The cell cycle and mRNA expression of related genes after 24 hours，3 days and 7 days were detected by flow cytometry and real-time fluorescent quantitative polymerase chain reaction. Results The proportion of cells in G0/G1 phase after 24 hours and 3 days were higher in the exposure group than in the control group［（70.33±2.03）% vs. （46.90±2.65）%；（52.97±1.26）% vs. （46.87±1.85）%；all P<0.01］；there was no significant difference in the proportion of cells in G0/G1 phase between the two groups after 7 days（P>0.05）. The mRNA expression levels of branched chain amino acid transferase 1 （BCAT1）， cyclin-dependent kinases （CDKN1A） and chromatin licensing and DNA replication factor 1（CDT1）in the exposure group were up-regulated in 24 hours and were down-regulated in 3 days after detoxication （all P<0.05）；there was no significant difference in mRNA expression levels between the two groups after 7 days（P>0.05）. Conclusio Hexavalent chromium exposure could influence the cell cycle by modifying the mRNA expression levels of BCAT1，CDKN1A and CDT1.
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Published: 26 September 2018
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