|
|
|
| Association of UCA1 expression and rs7255437 polymorphism in UCA1 promoter region with colorectal cancer |
| LI Ying-jun*，LIU Bing，JING Fang-yuan，DING Ye，HE Qing-fang，ZHONG Yao-hong，FAN Chun-hong
|
| *Hangzhou Medical College，Hangzhou，Zhejiang 310053，China |
|
|
|
|
Abstract Objective To investigate the association of genetic variants in the promoter region of UCA1 and its expression level with colorectal cancer，especially to provide basis for UCA1 as a molecular biomarker for colorectal cancer risk. Methods Totally 507 cases of primary colorectal cancer and 503 healthy volunteers were included in the case group and the control group. The single nucleotide polymorphisms of the UCA1 rs7255437 locus were detected in the blood，and the association between the UCA1 rs7255437 polymorphism and the risk of colorectal cancer was analyzed. Bioinformatics method was used to verify the association between UCA1 expression and UCA1 rs7255437 polymorphism with risk of colorectal cancer. Results The results showed that compared with the CC genotype，CT and TT genotypes had an increased risk of colorectal cancer，however the difference was not statistically significant（P>0.05） . Compared with the CC genotype，TT genotype had a 67% decreased risk of advanced colorectal cancer（P<0.05） . The result of bioinformatics analysis showed that the UCA1 expression level of CT and TT carriers in normal transverse colon and small intestinal tissues was higher than that of CC carriers，and the expression level of UCA1 in colorectal cancer tissues in advanced colorectal cancer patients was higher than that of early colorectal cancer patients.Conclusion UCA1 expression and rs7255437 polymorphism are potential molecular markers for the development of colorectal cancer. However，confirmation studies with larger sample size and further mechanistic investigations into the function of UCA1 and its genetic variants are needed.
|
|
Published: 04 September 2018
|
|
|
|
|
|
[1] TORRE L A，BRAY F，SIEGEL R L，et al. Global cancer statistics，2012[J] . CA Cancer J Clin，2015，65（2）：87-108.
[2] CHEN W，ZHENG R，BAADE P D，et al. Cancer statistics in China，2015[J] . CA Cancer J Clin，2016，66（2）：115-132.
[3] SIEGEL R L，MILLER K D，FEDEWA S A，et al. Colorectal cancer statistics，2017[J] . CA Cancer J Clin，2017，67（3）：177-193.
[4] BROSNAN C A，VOINNET O. The long and the short of noncoding RNAs[J] . Curr Opin Cell Biol，2009，21（3）：416-425.
[5] LEE J T. Epigenetic regulation by long noncoding RNAs[J] . Science，2012，338（6113）：1435-1439.
[6] LI Y，BAO C，GU S，et al. Associations between novel genetic variants in the promoter region of MALAT1 and risk of colorectal cancer[J] . Oncotarget，2017，8（54）：92604-92614.
[7] WANG X S，ZHANG Z，WANG H C，et al. Rapid identification of UCA1 as a very sensitive and specific unique marker for human bladder carcinoma[J] . Clin Cancer Res，2006，12（16）：4851- 4858.
[8] HAN Y，YANG Y N，YUAN H H，et al. UCA1，a long non-coding RNA up-regulated in colorectal cancer influences cell proliferation，apoptosis and cell cycle distribution[J] . Pathology，2014，46（5）：396-401.
[9] JIN M，GU S，YE D，et al. Association between genetic variants in the promoter region of a novel antisense long noncoding RNA RP11-392P7.6 and colorectal cancer risk[J] . Environ Mol Mutagen， 2017，58（6）：434-442.
[10] LING H，SPIZZO R，ATLASI Y，et al. CCAT2，a novel noncoding RNA mapping to 8q24，underlies metastatic progression and chromosomal instability in colon cancer[J] . Genome Res，2013， 23（9）：1446-1461.
[11] GTEx Consortium. Human genomics. The Genotype-Tissue Expression （GTEx） pilot analysis：multitissue gene regulation in humans[J] . Science，2015，348 （6235）：648-660.
[12] 张晓怡，沈毅，韩雅斌，等. 桐乡市常见恶性肿瘤危险因素分析[J] . 浙江预防医学，2014，26（2）：150-153.
[13] RIM S H，SEEFF L，AHMED F，et al. Colorectal cancer incidence in the United States，1999-2004 ：an updated analysis of data from the National Program of Cancer Registries and the Surveillance，Epidemiology，and End Results Program[J] . Cancer，2009，115（9）：1967-1976.
[14] DE VOOGHT K M，VAN WIJK R，VAN SOLINGE W W. Management of gene promoter mutations in molecular diagnostics[J] . Clin Chem，2009，55（4）：698-708.
[15] WANG Y H，WANG F，ZHANG L，et al. Long non-coding RNA UCA1 can predict tumor lymph node metastasis[J] . Tumour Biol，2017，39（5）：1010428317706208.
[16] BIAN Z，JIN L，ZHANG J，et al. LncRNA-UCA1 enhances cell proliferation and 5-fluorouracil resistance in colorectal cancer by inhibiting miR-204-5p[J] . Sci Rep，2016（6）：23892.
[17] NI B，YU X，GUO X，et al. Increased urothelial cancer associated 1 is associated with tumor proliferation and metastasis and predicts poor prognosis in colorectal cancer[J] . Int J Oncol，2015，47（4）：1329-1338. |
|
|
|
