|
|
|
| Polymorphism of MTHFR C677T gene and MTHFR A1298C gene among physical examinees in Taizhou City |
| GU Wan-hong，WANG Pan，ZHONG Qian-yi，YANG Ying-mei
|
| Taizhou Central Hosptial，Taizhou，Zhejiang 318000，China |
|
|
|
|
Abstract Objective To explore the distribution of methylenetetrahydrofolate reductase（MTHFR）C677T gene and MTHFR A1298C gene polymorphism among 1 644 physical examinees in Taizhou region.Methods Fluorescence quantitative PCR method was used to detect MTHFR C677T and MTHFR A1298C genotype in 1 644 physical examinees from Taizhou Central Hospital from September 2016 to September 2017. According to the characteristics of gender，the distribution features of MTHFR C677T and MTHFR A1298C genotype were described，and then were compared with data about the physical examinees in other cities in China.Results Among the 1 644 physical examinees，MTHFR 677CC，677CT and 677TT genotype frequency were 40.09%，44.53%and 15.39% respectively and MTHFR 677 allele frequency was 37.65%. MTHFR 1298AA，1298AC and 1298CC genotype frequency were 65.69%，30.47% and 3.83% respectively and MTHFR 1298 allele frequency was 19.07%. Statistical significance was found in genotype distribution of MTHFR C677T between males and females（P=0.036）， and no statistical significance was found in genotype distribution of MTHFR A1298C between males and females（P=0.278）. As compared with the physical examinees in Henan，Wulumuqi and Beijing regions，there were statistically significance differences in the distribution of MTHFR C677T genotype and allele frequencies in Taizhou region（P<0.05）.Conclusion The distribution of MTHFR C677T gene polymorphism among physical examinees in Taizhou region is affected by gender，and the results shows certain regional specificity.
|
|
Published: 26 March 2018
|
|
|
|
|
|
[1] 赵加洪，李瑛，鲁衍强，等. MTHFR基因多态性检测在生殖健康领域的临床价值[J] . 中国妇幼卫生杂志，2015（5）：79-83.
[2] NASR A，IBRAHIM N，SAMI R. Methylenetetrahydrofolate reductase gene polymorphisms（677C>T and 1298A>C）in Egyptian patients with non-hodgkin lymphoma[J] . Journal of Cancer Research and Therapeutics，2012，8（3）：355.
[3] JIA J，MA Z，WU S. Positive association between MTHFR C677T polymorphism and oral cancer risk：a meta-analysis[J] . Tumour Biol，2014，35（5）：4943-4948.
[4] YANG Y M，ZHANG T T，YUAN L，et al. The association between the C677T polymorphism in MTHFR gene and the risk of thyroid cancer：a meta-analysis[J] . Eur Rev Med Pharmacol Sci，2014，18（15）：2097-2101.
[5] ZHANG M J，LI J C，YIN Y W，et al. Association of MTHFR C677T polymorphism and risk of cerebrovascular disease in Chinese population：an updated meta-analysis[J] . J Neurol，2014，261（5）：925-935.
[6] YU L，CHANG K，HAN J，et al. Association between Methylenetetrahydrofolate reductase C677T polymorphism and susceptibility to cervical cancer：a meta-analysis[J] . PLoS ONE，2013，8（2）：e55835.
[7] 任玮，李艳，童永清，等. 武汉地区353例体检者MTHFR C677T基因多态性分布[J] . 实用预防医学，2016，23（8）：1007-1010.
[8] 吕雪，徐金义，李涛，等. 河南汉族人群MTHFR基因多态性及同型半胱氨酸与冠心病关系研究[J] . 中华实用诊断与治疗杂志，2015，29（6）：603-604，607.
[9] 郝友瑛，鲁衍强，李瑛，等. 乌鲁木齐市汉族人群叶酸代谢通路关键酶基因MTHFR、MTRR单核苷酸多态性分布特征调查[J] . 中国妇幼保健，2016，31（18）：3772-3775.
[10] 冯琳琳，张微，张浩，等. 体检人群血清同型半胱氨酸水平与MTHFR C677T基因型频率的调查[J] . 中国卫生检验杂志，2015，25（13）：2225-2226，2229.
[11] 费方荣，胡如英，钟节鸣，等. 浙江省成年人健康期望寿命分析[J] . 预防医学，2017，29（10）：973-976.
[12] 李刚.叶酸联合VitB12对高血压伴同型半胱氨酸血症患者颈动脉硬化的影响[J] .中国高血压杂志，2015，2（23）：182- 184.
[13] ZHU X L，LIU Z Z，YAN S X，et al. Association between the MTHFR A1298C polymorphism and risk of cancer：evidence from 265 case-control studies[J] . Mol Genet Genomics，2016，291（1）：51-63.
[14] COPPED F.The genetics of folate metabolism and maternal risk of birth of a child with Down syndrome and associated congenital heart defects[J] . Front Genet，2015，25（6）：223.
[15] 华知奋，黄彩梅，鲁衍强，等. MTHFR基因多态性与原因不明复发性流产的相关性研究[J] . 国际检验医学杂志，2017，38（1）：16-17.
[16] NAIR R R，KHANNA A，SINGH R，et al. Association of maternal and fetal MTHFR A1298C polymorphism with the risk of pregnancy loss：a study of an Indian population and a meta-analysis[J] . Fertil Steril，2013，99（5）：1311-1318. |
|
|
|
