Abstract：Objective To evaluate the health effects of persistent organic pollutants（POPs）on body weight,food intake,internal organs,blood biochemistry,metabolic enzymes and antioxidant ingredients of rats. Methods Sixteen healthy Sprague-Dawley（SD）rats were randomly divided into the experimental group exposed to 10 mL/kg mixture of POPs（10 ng/mL PCBs,5 ng/mL PBDEs,1 ng/mL PCDD/F）everyday for 28 days by gavage,and the control group exposed to the same volume of soybean oil in the same way. Body weight and food intake of the rats were recorded regularly;blood routine and biochemical indices were detected;liver,kidney,spleen and testicles（ovary）of the rats were weighed to calculate organ coefficients;metabolic enzymes and antioxidant ingredients were detected from livers of the rats. Results No obviously abnormal symptoms and no deaths were found in both groups. Compared to the control group,the weekly food intake in the experimental group increased more for there was an interaction between grouping and time（P< 0.05）. The ratio of liver to body weight of male rats in the experimental group was higher than that in the control group [（3.87± 0.19）% vs.（3.53± 0.06）%,P< 0.05]. The haemoglobin and red blood cell of female rats in the experimental group were lower than those in the control group[（145.25± 6.18）g/L vs.（154.50± 4.20）g/L;（6.90± 0.14）× 1012/L vs.（7.39± 0.24）× 1012/L;both P< 0.05]. The glutathione-S-transferase（GST）of female rats in the experimental group was higher than that in the control group [（13.37± 1.05）U/mgprot vs.（9.43± 1.08）U/mgprot,P< 0.05]. The cytochrome P4501A1of rats in the experimental group was higher than that in the control group [female：（88.23± 5.81）ng/mgprot vs.（73.85± 5.86）ng/mgprot;male：（96.80± 13.32）ng/mgprot vs.（ 72.20± 2.01）ng/mgprot;both P< 0.05]. Conclusion After exposed to low dose of POPs,the cytochrome P4501A1 increased in all rats,the liver to body weight ratio increased in male rats,GST activity increased while red blood cell and haemoglobin decreased in female rats,which indicated possible body damages in rats.
KATSOYIANNIS A,CINCINELLI A.On persistent organic pollutants in Italy-from Seveso to the Stockholm Convention and beyond[J]. Sci Total Environ,2017,579:514-516.
ZHANG J,JIANG Y,ZHOU J,et al.Elevated body burdens of PBDEs,dioxins,and PCBs on thyroid hormone homeostasis at an electronic waste recycling site in China[J]. Environ Sci Technol,2010,44(10):3956-3962.
[6]
XU P P,LOU X M,DING G X,et al.Association of PCB,PBDE and PCDD/F body burdens with hormone levels for children in an e-waste dismantling area of Zhejiang Province,China[J]. Sci Total Environ,2014,499:55-61.
[7]
AIRAKSINEN R,HALLIKAINEN A,RANTAKOKKO P,et al.Time trends and congener profiles of PCDD/Fs,PCBs,and PBDEs in Baltic herring off the coast of Finland during 1978-2009[J]. Chemosphere,2014,114:165-171.
[8]
ROSZKO M,SZYMCZYK K,JEDRZEJCZAK R.Influence of hen breeding type on PCDD/F,PCB & PBDE levels in eggs[J]. Sci Total Environ,2014,487:279-289.
[9]
PLOTEAU S,ANTIGNAC J P,VOLTEAU C,et al.Distribution of persistent organic pollutants in serum,omental,and parietal adipose tissue of French women with deep infiltrating endometriosis and circulating versus stored ratio as new marker of exposure[J]. Environ Int,2016,97:125-136.
[10]
KNUDSEN A S,LONG M,PEDERSEN H S,et al.Persistent organic pollutants and haematological markers in Greenlandic pregnant women:the ACCEPT sub-study[J]. International journal of circumpolar health,2018,77(1):2242-3982.
[11]
BRUCHAJZER E,FRYDRYCH B,SPORNY S,et al.Toxicity of penta- and decabromodiphenyl ethers after repeated administration to rats:a comparative study[J]. Arch Toxicol,2010,84(4):287-299.
[12]
BRUCHAJZER E,FRYDRYCH B,KILANOWICZ A,et al.Selected oxidative stress parameters after single and repeated administration of octabromodiphenyl ether to rats[J]. Int J Occup Med Environ Health,2014,27(5):808-820.
SRIDHAR M,VENKATARAMAN P,DHANAMMAL S,et al.Impact of polychlorinated biphenyl(Aroclor 1254 )and vitamin C on antioxidant system of rat ventral prostate[J]. Asian J Androl,2004,6(1):19-22.
[15]
OBERG M,WESTERHOLM E,FATTORE E,et al.Toxicity of Bromkal 70-5DE,a technical mixture of polybrominated diphenyl ethers,following 28 d of oral exposure in rats and impact of analysed impurities[J]. Chemosphere,2010,80(2):137-143.
[16]
BRENEROVÁ P,HAMERS T,KAMSTRA J H,et al.Pure non-dioxin-like PCB congeners suppress induction of AhR- dependent endpoints in rat liver cells[J]. Environ Sci Pollut Res Int,2016,23(3):2099-2107.
[17]
RATHORE R,SCHRAMM K W. Ethoxyresorufin-O-deethylase(EROD)activity modulation of 2,3,7,8-tetrachlorodibenzo-p-dioxin and 3,3',4,4',5-pentachlorobiphenyl(PCB 126)in the presence of aqueous suspensions of nano-C60[J]. Altern Lab Anim,2014,42(1):71-80.
[18]
O'KANE A A,ELLIOTT C T,MOONEY M H. Complex interactions between dioxin-like and non-dioxin-like compounds for in vitro cellular responses:implications for the identification of dioxin exposure biomarkers[J]. Chem Res Toxicol,2014,27(2):178-187.
