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预防医学  2020, Vol. 32 Issue (4): 361-365    DOI: 10.19485/j.cnki.issn2096-5087.2020.04.009
  综述 本期目录 | 过刊浏览 | 高级检索 |
新型冠状病毒实验室检测方法研究进展
张友春1,汪睿2综述,朱永良2审校
1.淳安县妇幼保健院,浙江 淳安 311700;
2.浙江大学医学院附属第二医院
Research progress of laboratory testing methods for SARS-CoV-2
ZHANG Youchun*, WANG Rui, ZHU Yongliang
*Maternal and Child Health Hospital of Chun'an,Chun'an,Zhejiang 311700,China
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摘要 新型冠状病毒(SARS-CoV-2)正在世界范围内暴发流行,导致人类严重呼吸道疾病且致死率高,引起全球的高度关注。该病毒造成的新型冠状病毒肺炎(COVID-19)尚无特效治疗药物,早期诊断是当前主要的防控措施。依据《新型冠状病毒肺炎诊疗方案(试行第七版)》,病毒核酸检测阳性、基因测序高度同源或血清学抗体检测阳性均可确诊SARS-CoV-2感染,然而各种检测方法均存在一定的假阴性率和潜在的假阳性结果。本文对目前正在应用和报道的SARS-CoV-2实验室检测方法,如病毒分离、二代测序技术、反转录-聚合酶链式反应、恒温核酸扩增技术、基因芯片和抗体检测等进行综述,并探讨各种方法的优缺点,为优化SARS-CoV-2感染诊断提供参考。
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ZHANG Youchun
WANG Rui
ZHU Yongliang
关键词 新型冠状病毒病毒分离核酸检测抗体检测    
Abstract:The outbreak of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)all over the world leads to serious respiratory diseases and high mortality,which arouses wide concern. No specific treatment for coronavirus disease 2019(COVID-19)caused by the virus has been found,so early diagnosis is the main prevention and control measure at present. According to the COVID-19 Diagnosis and Treatment Plan(Seventh Trial),Being positive in nucleic acid testing or in antibody testing,or being highly homologous in gene sequencing can confirm SARS-CoV-2 infection. However, all these detection Methods have potential false negative and false positive Results. This article summarizes the SARS-CoV-2 laboratory testing Methods that are being applied and studied at present,such as virus isolation,second generation sequencing,reverse transcription-polymerase chain reaction,nucleic acid isothermal amplification,gene chip and antibody detection,and discusses their advantages and disadvantages to optimize the diagnosis of SARS-CoV-2 infection.
Key wordssevere acute respiratory syndrome coronavirus 2    virus isolation    nucleic acid detection    serological detection
收稿日期: 2020-03-01      出版日期: 2020-04-16
ZTFLH:  R446  
  R563.1  
通信作者: 朱永良,E-mail:ylzhu@zju.edu.cn   
作者简介: 张友春,本科,副主任检验师,主要从事临床检验工作
引用本文:   
张友春,汪睿 综述,朱永良 审校. 新型冠状病毒实验室检测方法研究进展[J]. 预防医学, 2020, 32(4): 361-365.
ZHANG Youchun, WANG Rui, ZHU Yongliang. Research progress of laboratory testing methods for SARS-CoV-2. Preventive Medicine, 2020, 32(4): 361-365.
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http://www.zjyfyxzz.com/CN/10.19485/j.cnki.issn2096-5087.2020.04.009      或      http://www.zjyfyxzz.com/CN/Y2020/V32/I4/361
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