|
|
|
| Mycoplasma pneumoniae infection and drug resistance among children in Fuyang District |
| LUO Wen-long*, YANG Yu, HAN Xu-fang, ZHENG Yin-na
|
| *Clinical Laboratory of Hangzhou Fuyang Women and Children Hospital,Hangzhou,Zhejiang 311400,China |
|
|
|
|
Abstract Objective In order to investigate the Mycoplasma pneumonia（MP）infection and drug resistance in children in Fuyang,Hangzhou,and to provide a rational basis for the clinical diagnosis and treatment of MP in children. Methods A total of 1104 respiratory tract samples were collected from children patients with pneumonia. MP and its mutations in 23S rRNA gene locus were detected by real-time PCR. Then analyze pertinence between the clinical data and experimental results were conducted. Results Among 1104 respiratory tract samples,175 cases（15.85%）were MP positive. Moreover,the mutation of 23S rRNA gene locus existed in 115 MP positive samples with the positive infection rate of 65.71%. The infection rate （25.74%）and mutation rate（82.69%）in summer（Jul.-Sep.）was higher than other seasons,and the infection rate （11.72%）and mutation rate（40.63%）was the lowest in winter（Jan.-Mar.）. The infection rates of male and female children were 66.67% and 64.56%,and the mutation rates of male and female children were 66.67% and 64.56%. Conclusion MP infection may be related with climate and age. 23S rRNA gene locus mutation has a higher detection rate,prompting that MP shows high resistant rate to macrolides.
|
|
Revised: 21 November 2017
Published: 06 March 2018
|
|
|
|
|
|
[1] 吴倩,吴起武. 儿童肺炎支原体感染流行病学的研究进展[J]. 中国妇幼保健,2016,31(5):1111-1114.
[2] CHEN Z R,YAN Y D,WAN Y Q,et a1.Epidemiology of community acquired Mycoplasma pneumoniae respiratory tract infections among hospitalized Chinese children,including relationships with meteorological factors[J]. Hippokratia,2013, 17(1):20-26.
[3] 阳爱梅,宋建辉,黄榕,等. 1026例儿童肺炎支原体感染及耐药情况分析[J]. 中国当代儿科杂志,2013,15(7):522-525.
[4] 吕建海,唐兰芳. 儿童肺炎支原体肺炎临床特征分析[J]. 浙江预防医学,2016,28(3):310-312.
[5] PRINCIPI N,ESPOSITO S.Macrolide-resistant Mycoplasma pneumoniae:its role in respiratory infection[J]. Journal of Antimicrobial Chemotherapy,2013,68(3):506-511.
[6] MOROZUMI M,TAKAHASHI T,UBUKATA K.Macrolide-resistant Mycoplasma pneumoniae :characteristics of isolates and clinical aspects of community-acquired pneumonia[J]. Journal of Infection & Chemotherapy,2010,16(2):78-86.
[7] 姚慧生,张睿,刘立云,等. 肺炎支原体耐药基因检测与难治性肺炎支原体肺炎的相关性分析[J]. 国际儿科学杂志,2016,43(6):492-496.
[8] 孔庆东. 小儿肺炎支原体感染1079例及耐药分析[J]. 中国社区医师,2015,31(7):16-20.
[9] 胡亚美. 诸福棠实用儿科学[M]. 北京:人民卫生出版社,2005.
[10] 陈正荣,严永东. 小儿肺炎支原体感染流行病学特征[J]. 中国实用儿科杂志,2015,30(3):180-183.
[11] XU Y C,ZHU L J,XU D,et al.Epidemiological characteristics and meteorological factors of childhood Mycoplasma pneumoniae pneumonia in Hangzhou[J]. World J Pediatr,2011,7(3):240-244.
[12] 嵇龙飞,蔡云祥,张佩,等. 湖州市儿童肺炎支原体感染流行病学特征[J]. 预防医学,2017,29(11):1165-1166,1169.
[13] KASHYAP B,KUMAR S,SETHI G R,et al.Comparison of PCR,culture & serological tests for the diagnosis of Mycoplasma pneumoniae in community-acquired lower respiratory tract infections in children[J]. Indian Journal of Medical Research,2008,128(2):134.
[14] 陈晓颖,万莉雅,董汉权,等. 天津地区2008—2009年肺炎支原体肺炎的临床特点及治疗[J]. 中国妇幼保健,2012,27(23):3593-3595.
[15] 黄海樱,陈波,周强. 广州地区肺炎支原体感染儿童血清流行病学调查分析[J]. 中国儿童保健杂志,2014,22(1):83-85.
[16] 吴建荣,骆益华,骆勇. 2 595例呼吸道感染儿童肺炎支原体抗体检测结果[J]. 浙江预防医学,2016,28(5):518-519.
[17] 张淑艳,刘杰,赵满仓. 北京地区儿童肺炎支原体感染的流行病学分析[J]. 标记免疫分析与临床,2015,22(7):620-623.
[18] ZHAO F,LYU M,TAO X,et al.Antibiotic sensitivity of 40 Mycoplasma pneumoniae isolates and molecular analysis of macrolide-resistant isolates from Beijing,China[J]. Antimicrobial Agents & Chemotherapy,2012,56(2):1108-1109.
[19] 潘芬,张泓. 肺炎支原体耐药性及分子流行病学研究进展[J]. 上海交通大学学报(医学版),2014,34(8):1248-1253.
[20] 王良玉,辛德莉. 肺炎支原体感染实验室诊断的研究进展[J]. 传染病信息,2017,30(1):51-55. |
|
|
|
