|
|
|
| Application of Raman spectroscopy in selecting potential cleavage embyos |
| XU Wei-hai*, ZHANG Ling, ZHANG Lin, LI Shi-shi, SHU Jing
|
| *Zhejiang Provincial People's Hospital,Hangzhou,Zhejiang 310014,China |
|
|
|
|
Abstract Objective To develop a method with Raman spectroscopy for selecting embryos with high developing potential. Methods A total of 100 samples of D3 spent cultural medium for embryos assigned to blastocyst culture were collected,and the Raman spectroscopy produced with optimal integration time. The original spectrums retrieved were processed by filtration,baseline correction and normalization methods to form characteristic spectral for the spent medium of cleavage embryo,suitable for analysis. A further 173 samples were collected to analyze the clinical relevance of Raman spectroscopy in selecting the potential embryos. Results Original Raman spectra with good resolution can be obtained with an integral time over 240 seconds. The characteristic spectral shift of embryo culture medium was 942,1 346,1 456 and 1 656 cm-1 after processing. The spectral shift between the positive curve and the negative model was 1 100-1 800 cm-1. There was no significant difference in the intensity of real Raman spectral with different shifts（P>0.05）. A total of 80 positive curves,70 negative curves,23 neutral curves were obtained from the 173 D3 samples,and the blastocyst formation rates were 85.00%,22.86% and 65.22%,respectively,with statistically significant difference（P<0.001）. The sensitivity,specificity and Kappa value for the Raman spectrum curve predicting the blastocyst formation were 83.8%,73.0% and 0.572,respectively. Conclusion A clear,real and comparable Raman spectrum model could be obtained by improving the spectral collection conditions and preprocessing the original Raman spectrum. The potential embryo screened by this method is moderately consistent with that of blastocyst.
|
|
Received: 09 August 2017
Revised: 23 October 2017
Published: 01 January 1900
|
|
|
|
|
|
[1] NIINIMAKI M,VELEVA Z,MARTIKAINEN H. Embryo quality is the main factor affecting cumulative live birth rate after elective single embryo transfer in fresh stimulation cycles[J]. Eur J Obstet Gynecol Reprod Biol,2015,194：131-135.
[2] 徐维海,张琳,李施施,等. 再生育人群的辅助生殖实验技术应用[J]. 预防医学,2017,29（1）：49-52.
[3] NASIRI N,EFTEKHARI-YAZDI P. An overview of the available methods for morphological scoring of pre-implantation embryos in in vitro fertilization[J]. Cell J,2015,16（4）：392-405.
[4] 李海静,金月琴,高萍萍. 极低出生体重儿出生状况分析[J]. 预防医学,2017,29（5）：518-520,523.
[5] DING J,XU T,TAN X,et al. Raman spectrum： a potential biomarker for embryo assessment during in vitro fertilization[J]. Exp Ther Med,2017,13（5）：1789-1792.
[6] BRISON D,CALDERÖN G,CATT J,et al. The Istanbul consensus workshop on embryo assessment： proceedings of an expert meeting[J]. Hum Reprod,2011,26（6）：1270-1283.
[7] 许禄,邵学广.化学计量学方法[M].北京：科学出版社,2004：31-40.
[8] 张万里,朱键,李剑君,等. 基于自动线性拟合的快速拉曼基线校正算法[J]. 光谱学与光谱分析,2017,37 （8） ：2632- 2637.
[9] 王斌,戴连奎. 基于拉曼光谱解析的对二甲苯装置吸附塔循环液成分分析[J]. 光谱学与光谱分析,2015,35（2） ：399-403.
[10] 韦娜,冯叙桥,张孝芳,等. 拉曼光谱及其检测时样品前处理的研究进展[J]. 光谱学与光谱分析,2013,33（3）：694-698.
[11] GARDNER D K,WALE P L,COLLINS R,et al. Glucose consumption of single post-compaction human embryos is predictive of embryo sex and live birth outcome[J]. Hum Reprod,2011,26（8）：1981-1986.
[12] 邓彬,李予,王文军,等. 胚胎培养液氨基酸浓度与体外受精-胚胎移植妊娠结局的关系[J]. 中山大学学报（医学科学版）,2013 ,34（6）：900-905.
[13] YAGI A,MIYANAGA S,SHRESTHA R,et al. A fatty acid profiling method using liquid chromatography-high resolution mass spectrometry for improvement of assisted reproductive technology[J]. Clin Chim Acta,2016,456：100-106.
[14] GUO X Y,JIANG F,CHENG X J,et al. Embryonic soluble human leukocyte antigen-G as a marker of embryo competency in assisted reproductive technology for Chinese women[J]. J Reprod Med,2013,58（11-12）：477-84.
[15] 王海英,张仁礼,韩冬,等. 人胚胎源性人绒毛促性腺激素在胚胎培养液中的水平与早期胚胎发育的关系[J]. 南方医科大学学报,2014,34（7）：1039-1041.
[16] KUBELKA J. Multivariate analysis of spectral data with frequency shifts：application to temperature dependent infrared spectra of peptides and proteins[J]. Anal Chem,2013,85（20）：9588- 9595.
[17] 徐龙,罗永道. 光谱图像的标准具效应[J]. 云南师范大学学报（自然科学版）,2015,35（5）：44-47.
[18] 王欢,王永志,赵瑜,等. 拉曼光谱中荧光抑制技术的研究新进展综述[J]. 光谱学与光谱分析,2017,37（7）：2050-2056.
[19] ISHIGAKI M,HASHIMOTO K,SATO H,et al. Non-destructive monitoring of mouse embryo development and its qualitative evaluation at the molecular level using Raman spectroscopy[J]. Sci Rep,2017,7：43942.
[20] ZHAO Q,YIN T,PENG J,et al. Noninvasive metabolomic profiling of human embryo culture media using a simple spectroscopy adjunct to morphology for embryo assessment in in vitro fertilization （IVF）[J]. International Journal of Molecular Sciences,2013,14（4）：6556-6570. |
|
|
|
