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| Astudyonthenoninvasiveprenataldiagnosisoffetal bloodtypebymaternalblood |
| WANG Zhen-yong, SHEN Guo-song, HE Ping-ya, ZHANG Su, ZHANG Ya-qin
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| MaternalandChinaHealthHospitalofHuzhouCity,Huzhou,Zhejiang,313000,China |
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Abstract ObjectiveTo explore the value of prenatal diagnosis of fetal ABO blood groups in the prevention of ABO-HDN, and to provide evidence for prevention of ABO-HDN.MethodsA total of 3 777 samples were collected from the pregnant women whose ABO blood group is O, and we detected the ABO blood group by serological method to detect the titer of IgG anti-A and anti-B in the maternal blood.ResultsAmong the 3 777 samples collected from the pregnant women whose ABO blood group is O , the titer of IgG anti-A to anti-B was 1to1 024 in 27 samples(0.7%), 1∶512 in 97 samples(2.6%), 1∶256 in 163 samples(4.3%), 1∶128 in 285 samples(7.5%) and 1:64 in 603 samples(16%).We followed the pregnancy and newborn outcome of 769 case whose antibody titer of 1∶64 or more , and compared the fetal ABO blood group with results of the titer of IgG anti-A and/or anti-B. A total of 641 patients (83.3%) was corresponding resistance against A or B, and 128 patients (16.6%) was not corresponding resistance against A or B. The higher the antibody titer, the higher incidence of neonatal ABO hemolytic disease occurred. We extracted the fetal free DNA of peripheral blood plasma in 30 pregnant women, and the genotypes of fetal ABO blood group were detected by the polymerase chain reaction-sequence specific primer (PCR-SSP), and all the experiment presented success. ConclusionThe titer of IgG anti-A to anti-B could be used to prevent the occurrence of hemolytic disease of newborn. Considering the interference factors, the fetal free DNA in the maternal circulation could be used to prenatally detect fetal ABO blood groups.
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Received: 09 October 2015
Revised: 22 January 2016
Published: 05 January 2018
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[1] 温洁新,马西霞,孙稔侠.O型血孕妇血清中IgG抗A(B)抗体效价与ABO新生儿溶血病的相关分析[J].广东医学,2010,31(11):1430-1431.
[2] 蔡振华,方伟桢,肖木洲.孕妇血型抗体IgG效价与ABO血型不合致新生儿溶血病的相关性分析[J].现代医药卫生,2014,30(19):2951-2952.
[3] 陈宇宁,陈华根,黄学斌,等.孕妇IgG抗A(B)效价监测及新生儿溶血病追踪调查[J].现代预防医学,2013,40(16):3012-3014.
[4] 刘妍,刘昕鸣,阮峥,等.O型血孕妇产前IgG抗体效价异常与新生儿溶血病发病关系的探讨[J].中国实用医药,2015,10(9):106-107.
[5] CALIXTO R, OSTRONOFF M, OSTRONOFF F,et al. Immune hemolysis after fludarabine-based reduced intensity conditioning and allogenetic PBSC transplantation for CML with mitor ABO in compatibility [J]. Ann Hematol,2012,91(2):295-297.
[6] 郭丽侠,杨宇艳.母婴同室新生儿黄疸的早期监测与干预措施[J].中国妇幼保健,2014,25(15):2363-2364.
[7] 黄衍锋,陈建霞,邹焕荣,等.动态监测孕期血型抗体效价在新生儿溶血病诊疗中的意义[J].国际检验医学杂志,2013,34(19):2063-2064.
[8] 马印图,李莉华,李丽君,等.栀黄口服液联合血浆置换早期干预治疗母婴血型不合新生儿溶血病[J].中国输血杂志,2013,26(10):993-996.
[9] 胡亚美,江载芳,诸福棠.实用儿科学[M].7版.北京:人民卫生出版社,2002:477.
[10] 岳亚飞,邱洪涛,赵亚娟,等.PCR-SSP法检测胎儿、新生儿ABO血型[J].西安交通大学学报(医学版),2003,24(4):403-405.
[11] 兰炯采,孟庆宝,张印则,等.ABO血型基因分型及应用[J].中国免疫学杂志,2002,18(6):430-433.
[12] LUN F M,CHIU R W,ALLEN K C,et al.Microfluidics digital PCR reveals a higher than expected fraction of fetal DNA in maternal plasma[J].Clin Chem,2008,54(10):1664-1672.
[13] CHAN Z B,YAN M,LEI Z,et al.Application of fetal DNA in matemal plasma for non—invasive prenatal diagnosis of fetal sex[J].Yi Chuan,2004,26(1):18-22.
[14] CHIM S S,TONG Y K,CHIU R W,et al.Detection of the placental epigenetic signature of the maspin gene in maternal plasma[J].Proc Natl Acad Sci USA,2005,102(41):14753-14758.
[15] BELLIDO M L, RADPOUR R, LAPAIRE O, et al. MALDI-TOF mass array analysis of RASSF1A and SERPINB5 methylation patterns in human placenta and plasma.[J]. Biol Reprod, 2010, 82(4):745-750.
[16] 柳爱华,储穆庭. 2 573例孕妇ABO血型抗体效价检测结果及分析[J].现代预防医学, 2011,38(1):49-51.
[17] 彭素琼,张术华,刘峰,等.血型抗体效价在孕期检测的必要性[J].检验医学与临床,2015,12(7):959-960.
[18] 夏爱军,樊文昕,张献清,等. IgG亚型检测对高效价抗体孕妇的临床意义[J].中国输血杂志,2014,27(2):182-183. |
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