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A study on the lymphocytes genetic damage of occupational population exposed to methyl tert-butyl ether |
XUE Hui-zhong, WANG Yu, GAO Ming, ZHANG Guo-zhen, ZHU Fu-qiang, TANG Xiao-ping, RUAN Zheng, QIAN Ya-ling, WU Nan-xiang
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Zhejiang Academy of Medical Sciences,Hangzhou,Zhejiang,300013,China |
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Abstract Objective To investigate the genetic toxicity of methyl tert-butyl ether (MTBE) to lymphocytes and to provide reference for establishing the occupational population exposure limit of MTBE. Methods The human B lymphocytes in the logarithmic growth phase were respectively exposed to MTBE at concentrations of 0,1.25,2.5,5,10 and 12.5 μmol/L. The percentage of tail DNA and Olive tail moment of human B lymphocytes were evaluated after 24 h exposure by using comet assay. Apoptosis was tested by Annexin V-FITC/PI double-staining flow cytometry. The contents of MDA and 8-OHdG,as well as GSH-Px activity were measured by ELISA kit. Sixty workers from a petrochemical factory in Zhejiang were selected as the occupational exposure population,and 55 non-occupational exposure workers were selected as the control population. And 5 mL heparin anticoagulant peripheral blood was collected,and the number of micronucleus of peripheral blood lymphocytes was measured by micronucleus test,and the percentages of tail DNA as well as Olive tail moment of peripheral blood lymphocytes were measured by comet assay. The contents of MDA and 8-OhdG and GSH-Px activity in peripheral blood plasma were measured by ELISA kit. Results After 24 h exposure,the percentage of tail DNA and Olive tail moment in human B lymphocytes at concentrations of 10-12.5 μmol/L were significantly higher than those in the control group(P<0.05),and the percentage of early apoptosis cells was significantly increased at concentrations of 10-12.5 μmol/L (P<0.01). The results of population-based study indicated no statistically significant difference in micronucleus positive rate,and the contents of MDA and 8-OhdG,and GSH-Px activity between the exposure group and the control group,but the Olive tail moment was significantly higher in the exposure group compared with the control group(P=0.000). Conclusion The results of vitro study showed that exposure to 10-12.5 μmol/L MTBE could cause genetic toxicity to human B lymphocytes. Olive tail moment of peripheral blood lymphocytes of occupational exposure workers was significantly higher than that of non-occupational exposure group.
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Received: 22 January 2017
Revised: 17 April 2017
Published: 17 October 2017
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[1] 梁林涵,彭晓武,郑晶,等. 南宁市普通人群甲基叔丁基醚暴露健康风险评价[J]. 环境与健康杂志,2014,31(12):1073-1075. [2] 石宝明,王宇宁,白雪松. 2014年我国石油市场特点及2015年展望[J]. 当代石油石化,2015,23(1):12-17. [3] 江淑仪,陈小玲,方昌勇,等. 甲基叔丁基醚对小鼠外周血淋巴细胞的遗传毒性[J]. 卫生研究,2011,40(3):391- 393. [4] BELPOGGI F,SOFFRITTI M,MALTONI C. Methyl- tertiary- butyl ether (MTBE)—a gasoline additive—causes testicular and lymphohaematopoietic cancers in rats[J]. Toxicol Ind Health,1995,11(2):119-149. [5] BENSON J M,GIGLIOTTI A P,MARCH T H,et al. Chronic carcinogenicity study of gasoline vapor condensate(GVC)and GVC containing methyl tertiary-butyl ether in F344 rats[J]. J Toxicol Environ Health Part A,2011,74(10):638-657. [6] DODD D E,LAYKO D K,BERMUDEZ E. Methyl tertiary-butyl ether(MTBE):two-year combined chronic toxicity/carcinogenicity drinking water study in Wistar rats[R]. Protocol Number 07001.Research Triangle Park,NC:Hamner Institute for Health Sciences,2010. [7] Toxnet (Toxicology Data Network). HSDB: Methyl t-butyl ether[EB/OL]. [2016-07-26]. https://toxnet.nlm.nih.gov/cgi-bin/ sis/search2/r?dbs+hsdb:@term+@rn+1634-04-4. [8] 胡蔡松,周祖木,徐毅. 温州市放射工作人员健康状况调查[J]. 浙江预防医学,2015,27(2):179-181. [9] 张旺东,周琳艳,王秋菊,等. 低剂量电离辐射和适当干预措施对医务放射工作人员遗传物质影响的研究[J]. 国际检验医学杂志,2012,33(17):2075-2076. [10] CLARK C R,SCHREINER C A,PARKER C M,et al. Health assessment of gasoline and fuel oxygenate vapors:subchronic inhalation toxicity[J]. Regul Toxicol Pharmacol,2014,70(2 Suppl):S18-S28. [11] 李红艳,高锦伍,杨江,等. 甲基叔丁基醚的遗传毒性研究[J]. 劳动医学,2001,18(6):341-343. [12] 陈小玲,袁雪峰,徐聪兵,等. 甲基叔丁基醚诱导中国仓鼠肺成纤维细胞细胞周期改变及凋亡适应性反应[J]. 中国职业医学,2012,39(1):16-19. [13] 胡大林,彭晓武,彭晓春,等. 甲基叔丁基醚对加油站职业人群血清淋巴细胞DNA的损伤[J]. 环境与职业医学,2013,30(10):758-761. [14] 贺性鹏,蔡亚平,贺栋梁. 汽油添加剂甲基叔丁基醚对小鼠遗传毒性的作用[J]. 中国职业医学,2003,30(1):8-9. [15] SALIMI A,VAGHAR-MOUSSAVI M,SEYDI E,et al. Toxicity of methyl tertiary-butyl ether on human blood lymphocytes[J]. Environ Sci Pollut Res Int,2016,23(9):8556-8564. [16] 宋燕华,孟佳,徐彩菊,等. 原花青素的致突变性及抗氧化性实验研究[J]. 浙江预防医学,2014,26(6):552-556. [17] 陈苘,邹华,邢鸣鸾,等. 不同粒径纳米二氧化钛对大鼠氧化应激的影响[J]. 浙江预防医学,2012,24(11):1-3. |
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