|
|
Determination of pentachlorophenol in wooden chopping boards with gas chromatography-tandem mass spectrometry |
ZHAO Yuwen1, LI Zeran2, XUE Xiangju1, YANG Meng1, JI Wenliang2, RONG Weiguang2
|
1. Department of Foods, Shandong Drug and Food Vocational College, Weihai, Shandong 264210, China; 2. Institute of Physical and Chemical Detection, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu 210009, China |
|
|
Abstract Objective To optimize the determination of pentachlorophenol in wooden chopping boards through pretreatment of miniaturized samples. Methods The pretreated wooden chopping board samples were subjected to ultrasound extraction (1 mL of 0.5 mol/L K2CO3 added in 5 mL extraction solution) in 8 mL acetone and 2 mL water, followed by derivatization with 0.3 mL acetic anhydride, extraction with n-hexane and separation with DB-5ms column (30 m×0.25 mm, 0.25 μm). Gas chromatography tandem mass spectrometry (GC-MS/MS) was performed in multiple reaction monitoring (MRM) mode with quantitative analysis using the internal standard method. Results The GC-MS/MS assay showed a good linear relationship within the range of 0.01 to 0.2 µg/mL (R2>0.999), with a 0.003 mg/kg limit of detection and 0.01 mg/kg limit of quantitation. The mean recovery rates were 84.2% to 96.7% at spiked concentrations of 0.003, 0.01 and 0.03 mg/kg, with relative standard deviation of 2.2% to 6.1%. Conclusions The established GC-MS/MS assay is easy to perform, environment-friendly, highly accurate and sensitivity, which is feasible for determination of pentachlorophenol in wooden chopping boards.
|
Received: 04 May 2023
Revised: 06 July 2023
Published: 06 September 2023
|
|
|
|
|
[1] 丁先锋,方丰平,石文超,等.木材中五氯苯酚的检测方法研究进展[J].化学分析计量,2020,29(1):128-131. [2] 许文青,樊柏林,常薇,等.五氯苯酚和五氯苯酚钠毒性作用研究进展[J].中国药理学与毒理学杂志,2011,25(6):596-600. [3] 武丽辉. 24种农药产品被列入“高污染、高环境风险”产品目录[J].农药科学与管理,2008,29(6):54-55. [4] 沈宣铭,陈彤.气相色谱内标法测定皮革中五氯酚残留量不确定度评估[J].分析实验室,2008,27(7):102-105. [5] 巢猛,刘建明,陈明.SPE-HPLC法测定水中2,4,6-三氯酚和五氯酚[J].供水技术,2008,2(3):57-58. [6] 袁娅,周颖,熊丽,等.江西省食品接触用纸制品和竹木制品中五氯酚的污染状况调查[J].现代预防医学,2021,48(14):2538-2541. [7] 中华人民共和国国家卫生和计划生育委员会,中华人民共和国农业部,国家食品药品监督管理总局.食品安全国家标准动物源性食品中五氯酚残留量的测定液相色谱-质谱法:GB 23200.92—2016[S].北京:中国标准出版社,2016. [8] 中华人民共和国国家林业局.防腐木材和人造板中五氯苯酚含量的测定方法:LY/T 1985—2011[S].北京:中国标准出版社,2011. [9] 湖南省质量技术监督局.竹砧板安全技术要求:DB43/T 643—2011[S].2011. [10] 宋迎春,袁娅,王艳敏.GC-MS测定动物组织中五氯酚钠的不确定度评定[J].实验与检验医学,2019,37(4):568-572. [11] 雍宗锋,曹慧,蔡丹丹.全自动固相萃取-内标衍生-气相色谱-串联质谱法测定肉中五氯酚[J].江苏预防医学,2017,28(6):619-623. |
|
|
|