|
|
|
| Determination of antibiotic residues in chicken meat using ultra-high-performance liquid chromatography coupled with hybrid quadrupole-orbitrap high-resolution mass spectrometry |
| PAN Xiaodong, HAN Jianlong, CHEN Qing
|
| Department of Physicochemical and Toxicology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China |
|
|
|
|
Abstract Objective To optimize the sample pretreatment and establish an ultra-high-performance liquid chromatography coupled with hybrid quadrupole-orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap-MS) assay based on the parallel reaction monitoring (PRM) mode for determination of antibiotic residues in chicken meat. Methods Blank matrix-spiked chicken meat samples were extracted with 95% acetonitrile aqueous solution containing Na2EDTA and formic acid. The extraction solutions were cleaned up using different combinations of C18, PSA and GCB fillers, and the combinations with a higher antibiotic recovery rate was screened. The residues of 32 antibiotics were determined using UPLC-Q-Orbitrap-MS based on the PRM mode. Results If the extraction solution was cleaned up using the C18 filler, the largest number of antibiotics with a spiked recovery rate of >80% was seen, with matrix effects of 82.2% to 112.6%. The detection limits of 32 antibiotics were 0.8 to 5.8 μg/kg, with linear correlation coefficients of >0.99, spiked recovery rates of 71.3% to 111.5% and relative standard deviations of 3.2% to 14.2%. Conclusion The UPLC-Q-Orbitrap-MS assay is suitable for determination and quantitative analysis of multiple antibiotics in chicken meat.
|
|
Received: 04 January 2023
Revised: 17 April 2023
Published: 15 May 2023
|
|
|
|
|
|
[1] 陈兴连,李倩,王志飞,等.超高效液相色谱-串联质谱法同时测定鸡肉和鸡蛋中25种兽药残留[J].质谱学报,2021,42(6):1046-1058.
[2] 潘晓东,吴平谷,姜维.超高效液相色谱-串联质谱法同时测定鱼肉中氯霉素、甲砜霉素和氟甲砜霉素[J].中国食品卫生杂志,2014,26(6):572-574.
[3] 张弛,任君,陈达炜,等.分散微固相萃取-超高效液相色谱-高分辨质谱法测定火锅底料中罂粟壳生物碱[J].中国食品卫生杂志,2021,33(1):29-35.
[4] 唐晓琴,赵舰,贺丽迎,等.72种生物碱高分辨质谱数据库建立与应用[J].中国食品卫生杂志,2020,32(3):228-233.
[5] 潘晓东,黄百芬,张京顺,等.猪肉中16种β-内酰胺类抗生素UPLC-Q-Orbitrap检测方法研究[J].预防医学,2019,31(11):1100-1104.
[6] CHEN Q,PAN X D,HUANG B F,et al.Quantification of 16β-lactams in chicken muscle by QuEChERS extraction and UPLC-Q-Orbitrap-MS with parallel reaction monitoring[J].J Pharmaceut Biomed,2017,145:525-530.
[7] 刘善菁,宋慧敏,曲斌,等.液相色谱-四极杆-静电场轨道阱高分辨质谱法测定牛奶中8种氨基糖苷类抗生素残留[J].中国兽药杂志,2022,56(8):46-57.
[8] 韩梅,焦颖,郭灵安,等.QuEChERS-超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法同时测定生姜中21种喹诺酮类抗生素残留[J].食品安全质量检测学报,2021,12(23):9251-9259.
[9] 吴明媛,余焘,谢宗升,等.液相色谱-四极杆/静电场轨道阱高分辨质谱法快速筛查海水中大环内酯类抗生素[J].理化检验(化学分册),2021,57(5):444-449. |
|
|
|
