|
|
|
| Skin damage caused by sunlight ultraviolet in mice |
| XIA Yong, SONG Yanhua, XU Caiju, LU Wei, YAN Jun, CAI Delei, XIE Jiaying
|
| Department of Physicochemical and Toxicology, Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou, Zhejiang 310051, China |
|
|
|
|
Abstract Objective To study the damage effect of sunlight ultraviolet exposure on skin. Methods No exposure group, low exposure group and high exposure group were set up with 10 mice in each. The exposure doses of sunlight ultraviolet were 0, 10 J/cm2 and 20 J/cm2, respectively. The skin of mice was irradiated by a sunlight ultraviolet simulator for 5 days a week, 13 weeks. At the end of the experiment, the skin appearance of mice was examined; the skin moisture, oil content, texture density, hydroxyproline ( HYP ), hyaluronic acid (HA), malondialdehyde ( MDA ), glutathione ( GSH ) and superoxide dismutase ( SOD ) activities were detected; and the skin tissue morphology, collagen fiber morphology and elastic fiber morphology were observed. Results The skin appearance of mice in the no exposure group was normal; in the low exposure group, only one mouse had mild skin desquamation; in the high exposure group, the skin was loose and wrinkled, dry and desquamated, local thickening and erythema formation. Compared with the no exposure group, the contents of skin moisture, HYP, HA and SOD activity were lower, texture density, MDA content, morphological scores of skin tissue, collagen fiber tissue and elastic fiber tissue were higher in the high exposure group ( all P<0.05 ). Compared with the low exposure group, the HA content and SOD activity were lower, the skin texture density, MDA content, and histomorphological scores of skin tissue and collagen fibers were higher in the high exposure group ( all P<0.05 ). Conclusion Exposure to 20 J/cm2 sunlight ultraviolet can significantly lead to abnormal skin appearance and function in mice.
|
|
Received: 15 July 2021
Revised: 10 September 2021
Published: 12 January 2022
|
|
|
|
|
|
[1] DAVID M D,PATRICIA F,GIUSEPPE V.Atmospheric skin aging-contributors and inhibitors[J].J Cosmet Dermatol,2018,17:124-137.
[2] SAVIC S,SMILJIC S,LESTAREVIC S,et al.The structural characteristics of photoageing in mice caused by the effects of ultraviolet A radiation[J].Folia Morphol(Warsz),2019,79(3):548-556.
[3] 李世军,汪宇,张伟.贵阳地区190例正常人紫外线最小红斑量测定[J].贵州医科大学学报,2016,41(9):1075-1107.
LI S J,WANG Y,ZHANG W.Determination of minimal erythema dose of ultraviolet in 190 volunteers from Guiyang[J].J Guizhou Med Univ,2016,41(9):1075-1107.
[4] 郑红艳,杨丽,朱建宇,等.人工紫外线诱导皮肤参数变化的初步研究[J].香料香精化妆品,2020(5):53-55,85.
ZHENG H Y,YANG L,ZHU J Y,et al.A preliminary study on skin characteristic parameters after solar-simulated radiation[J].Flavour Fragrance Cosmetics,2020(5):53-55,85.
[5] 楼小航,张福仁,田洪青,等.济南地区94例正常人最小红斑量测定[J].中华皮肤科杂志,2006,39(6):361.
LOU X H,ZHANG F R,TIAN H Q,et al.Determination of minimum erythema volume in 94 normal subjects in Jinan[J].Chin J Dermatol,2006,39(6):361.
[6] 胡立文,高倩,徐文英,等.皮肤紫外线暴露剂量旋转人体模型方法测量[J].中国公共卫生,2012,28(9):1207-1209.
HU L W,GAO Q,XU W Y,et al.Skin ultraviolet exposure dosimetry using rotating manikin[J].Chin J Public Health,2012,28(9):1207-1209.
[7] 孙侠,刘香梅,庞增雄,等.不同时间紫外线照射对SD大鼠的皮肤光毒性损伤作用[J].实验动物与比较医学,2019,39(2):124-130.
SUN X,LIU X M,PANG Z X,et al.Phototoxic effects of ultraviolet irradiation at different times on skin of SD rats[J].Lab Anim Comp Med,2019,39(2):124-130.
[8] 朱彦君,孟宇宏,冯光珍,等.紫外线照射后无毛鼠皮肤胶原的变化及其定量分析[J].中华医学美学美容杂志,2005,11(3):159-162.
ZHU Y J,MENG Y H,FENG G Z,et al.Quantitative and quanlitative changes of collagen in ultraviolet irradiated skin of hairless mice[J].Chin J Med Aesth Cosmet,2005,11(3):159-162.
[9] 王鹏程,陈林莺,翁丹枫,等.3种不同染色方法对肺组织弹力纤维染色效果的影响[J].福建医科大学学报,2020,54(5):89-92.
WANG P C,CHEN L Y,WENG D F,et al.Effects of three different staining methods on the staining effect of elastic fibers in lung tissue[J]. J Fujian Med Univ,2020,54(5):89-92.
[10] BERNSTEIN E E,BROWN D B,SCHWARTZ M D,et al.The polyhydroxy acid gluconolactone protects skin against UV in an in vitro model of cutaneous photodamage[J].Dermatol Surg,2004,30(2):189-196.
[11] 司秋霞. 透明质酸钠改善皮肤水份状态的研究[J/CD].中西医结合心血管病电子杂志,2020,8(31):79-89.
SI Q X.Study on sodium hyaluronate improving skin moisture state[J/CD].Cardiovascu Dis J Integr Tradit Chin Western Med (Electronic),2020,8(31):79-89.
[12] 陈建澍,王婧茜,易喻,等.透明质酸及其衍生物研究进展[J].中国生物工程杂志,2015,35(2):111-118.
CHEN J S,WANG J Q,YI Y,et al.Research progress of hyaluronic acid and its derivatives[J].China Biotechnol,2015,35(2):111-118.
[13] 李张军,牛新武,肖生祥,等.小鼠皮肤光老化动物模型建立方法的改良[J].西安交通大学学报(医学版),2016,37(1):144-156.
LI Z J,NIU X W,XIAO S X,et al.Improvement of establishing skin photoaging model in mice[J].J Xi'an Jiaotong Univ (Med Sci),2016,37(1):144-156.
[14] YANG Y Q,YIN R,WU R Y,et al.DNA methylome and transcriptome alterations and cancer prevention by triterpenoid ursolic acid in UVB-induced skin tumor in mice[J].Mol Carcinog,2019,58(10):1738-1753.
[15] 刘金娟,杨宏发,李勇坚,等.紫外线对人皮肤成纤维细胞老化损伤的影响[J].中国免疫学杂志,2019,35(9):1053-1058.
LIU J J,YANG H F,LI Y J,et al.Effect of ultraviolet on aging injury of human skin fibroblasts[J]. Chin J Immunol,2019,35(9):1053-1058.
[16] 王燕华,蔡丽敏,杨晶,等.紫外线诱导人皮肤成纤维细胞形态改变及对基质金属蛋白酶表达的影响[J].基础医学与临床,2017,37(3):360-363.
WANG Y H,CAI L M,YANG J,et al.UVB induce morphological changes and the expression of MMPs in human fibroblasts[J].Basic Clin Med,2017,37(3):360-363.
[17] JUNG H O,YUNG H J,FATIH K,et al.Syringaresinol inhibits UVA-induced MMP-1 expression by suppression of MAPK/AP-1 signaling in haCaT keratinocytes and human dermal fibroblasts[J].Int J Mol Sci,2020,21(11):3981-3992.
[18] XU D F,WANG W Q,LIAO J M,et al.Walnut protein hydrolysates, rich with peptide fragments of WSREEQEREE and ADIYTEEAGR ameliorate UV-induced photoaging through inhibition of the NF-κB/MMP-1 signaling pathway in female rats[J].Food Funct,2020,11(12):10601-10616. |
|
|
|
