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| A study on the application of povidone-iodine diluent in intraoperative nursing of cervical cancer surgery |
| ZHOU Hui-fang, YANG Li-wei, HE Xu-jun, YU Ru-ping, XU Min, YUAN Mian
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| Zhejiang Provincial people＇s hospital, Hangzhou, Zhejiang, 310014, China |
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Abstract Objective To investigate the effect of povidone-iodine diluent on proliferation and apoptosis of cervical cancer cell HeLa and to provide the theoretical basis for its clinical application. Methods Human cervical cancer cell line HeLa in logarithmic growth phase were treated with different dilutions of povidone-iodine and the cells treated with physiological saline were set as the control group. The cells viability, morphological change, formation of apoptotic bodies, cell apoptosis and the apoptosis-related protein expression in HeLa cells were assessed by MTT assay, Hoechst33342 staining, AnnexinV/PI flow cytometry and Western blotting.Results Povidone-iodine diluent remarkably inhibited human cervical cancer cell line HeLa growth in a concentration-dependent manner. The inhibitory rates of HeLa cells were 25.3%, 30.8%, 33.4%, 60.3%, 71.2%, 85.3%, 89.1% and 91.2% when the concentration of povidone-iodine solution were 0.001%, 0.005%, 0.01%, 0.05%, 0.1%, 0.5%, 1% and 2%, respectively. The nuclear chromatin of HeLa cells treated with povidone-iodine dilution was agglutinated and contracted, and the nucleus was fragile and appeared apoptotic body, with dense and dense stain or fragment dense staining. With the increase of the concentration of povidone-iodine dilution,the apoptotic rate of HeLa cells increased,so were Caspase -8 , Caspase-3 and cleaved PARP.Conclusion Diluted povidone-iodine can strongly inhibit the proliferation of human cervical cancer cell line HeLa and the possible mechanism was the promotion of apoptosis.
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Received: 15 July 2016
Published: 21 November 2017
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