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| A study on the Xylo-oligosaccharides in combination with chitooligosaccharides in protecting mice from alcohol-induced liver injury and modulate immune function in mice |
| SONG Yan-hua, CAI De-lei, XIA Yong, LU Wei, FU Jian-yun, XU Cai-ju
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| Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou ,Zhejiang,310051,China |
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Abstract Objective To investigate effects of xylo-oligosaccharides with chitooligosaccharides on alcohol-induced liver injury and immune function in mice. Methods Different doses of chitosan oligosaccharide（0.045 g/kg-0.26 g/kgB.W）and xylo-oligosaccaride （0.055 g/kg-0.32 g/kgB.W） were feed to the mice for 30 days. The mice live-injury model was induced by alcohol. MDA、GSH、TG level in liver and T lymphocyte proliferation of spleen cells induced by conA,the antibody-producing cells,and natural killer（NK）activity were detected. Results Compared with the live-injury model group,MDA level of low/middle dose group,TG level of middle dose group and pathological evaluation score of liver steatosis of high dose group in mice liver were decreased because of chitosan oligosaccharide and xylo-oligosaccaride feeding. Compared with the control group,the ability of T lymphocyte proliferation of mouse spleen induced by ConA and the antibody-producing cells were increased in mice of middle and high dose group. The differences had statistical significances（P<0.05）. Conclusion Under this experimental condition,xylo-oligosaccharides in combination with chitooligosaccharides could protect the mice from alcohol-induced liver injury and enhance immune function in spleen of normal mice synergistically.
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Received: 28 September 2016
Revised: 23 December 2016
Published: 15 November 2017
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