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Concentration of target genes of SARS-CoV-2 and influencing factors in the confirmed cases with COVID-19 in Ningbo |
ZHANG Dandan, FU Xiaohong, JIAO Suli, GU Wenzhen, XU Guozhang
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Ningbo Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China |
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Abstract Objective To learn the concentration of target genes of severe acute respiratory syndrome coronavirus 2 ( SARS-CoV-2 ) and the influencing factors in the confirmed cases with coronavirus disease 2019 ( COVID-19 ) in Ningbo. Methods Demographic information and clinical diagnosis of COVID-19 cases reported in Ningbo from January 21 to February 20, 2020 were collected through China Disease Control and Prevention Information System. The Ct values of ORF1ab and N of the cases were collected through Ningbo Center for Disease Control and Prevention and designated hospitals, and analyzed in terms of gender, age, severity, clinical classification, source of case, sampling time and specimen type. Results There were 157 confirmed COVID-19 cases, including 56 males ( 35.67% ) and 101 females ( 64.33% ). Sixty-seven cases ( 42.68% ) aged between 40 and 60 years old. The Ct values of ORF1ab and N in females were 29.96±5.28 and 30.38±4.90, which were higher than 27.56±4.94 and 28.03±4.88 in males ( P<0.05 ). The Ct values of ORF1ab and N were the lowest when sampled at 1-7 days after onset ( P<0.05 ), which were 27.84±4.80 and 28.35±4.65. The Ct values of ORF1ab ( rs=0.288, P=0.001 ) and N ( rs=0.296, P=0.001 ) were positively correlated with sampling time. The Ct values of ORF1ab in pharyngeal swab and sputum were 29.19±4.85 and 28.74±6.40, the Ct values of N in pharyngeal swab and sputum were 29.61±4.60 and 29.22±6.10, both without significant differences between different samples ( P>0.05 ). Conclusions ampling time has a great influence on the Ct values of ORF1ab and N of SARS-CoV-2.
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Received: 11 June 2020
Revised: 10 September 2020
Published: 10 December 2020
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[1] 中华人民共和国国家卫生健康委员会.关于印发新型冠状病毒肺炎防控方案(第六版)的通知[EB/OL].(2020-03-07)[2020-09-10].http://www.nhc.gov.cn/jkj/s3577/202003/4856d5b0458141fa9f376853224d41d7.shtml. [2] World Health Organization.Coronavirus disease(COVID-19)outbreak situation[EB/OL].(2020-06-10)[2020-09-10].https://www.who.int/docs/default-source/coronaviruse/situation-reports/20200610-covid-19-sitrep-142.pdf?sfvrsn=180898cd_6. [3] 中国疾病预防控制中心新型冠状病毒肺炎应急响应机制流行病学组.新型冠状病毒肺炎流行病学特征分析[J].中华流行病学杂志,2020,41(2):145-151. [4] BAI Y,YAO L,WEI T,et al.Presumed asymptomatic carrier transmission of COVID-19[J].JAMA,2020,323(14):1406-1407. [5] 安娜,俸家富.医学检验技术在新型冠状病毒肺炎诊疗中的应用[J].检验医学与临床,2020,17(7):865-868,872. [6] 里进,叶光明,陈良君,等.新型冠状病毒核酸检测假阴性结果原因分析及对策[J].中华检验医学杂志,2020,43(3):221-225. [7] 王达,董梁,卿松,等.新型冠状病毒核酸检测中的思维误区[J].中华医院感染学杂志,2020,30(8):1167-1170. [8] CERAOLO C, GIORQI F M.Genomic variance of the2019-nCoV coronavirus[J/OL].J Med Virol(2020-02-06)[2020-09-10].https://www.ncbi.nlm.nih.gov/pubmed/?term=Genomic+variance+of+the+2019-nCoV+coronavirus.DOI:10.1002/jmv.25700. [9] 中华人民共和国国家卫生健康委员会.新型冠状病毒感染的肺炎防控方案(第二版)[Z/OL].(2020-01-22)[2020-09-10].http://health.cnr.cn/jkgdxw/20200123/t20200123_524947936.shtml. [10] 中华人民共和国国家卫生健康委员会. 新型冠状病毒感染的肺炎实验室检测技术指南(第二版)[Z/OL].(2020-01-22)[2020-09-10].https://max.book118.com/html/2020/0123/8000037012002074.shtm. [11] ZHAO J,YUAN Q,WANG H,et al.Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease2019[J/OL].Clin Infect Dis(2020-03-28)[2020-09-10].https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184337/pdf/ciaa344.pdf.DOI:10.1093/cid/ciaa344. [12] 中华预防医学会新型冠状病毒肺炎防控专家组.新型冠状病毒肺炎流行病学特征的最新认识[J].中华流行病学杂志,2020,41(2):139-144. [13] 陈奕,王爱红,易波,等.宁波市新型冠状病毒肺炎密切接触者感染流行病学特征分析[J].中华流行病学杂志,2020,41(5):667-671. [14] 高文静,郑可,柯骥,等.新型冠状病毒肺炎无症状感染相关研究进展[J].中华流行病学杂志,2020,41(7):990-993. [15] 程芳,刘赛朵,叶新春,等.新型冠状病毒肺炎普通型和重症病例临床特征比较[J].预防医学,2020,32(9):886-890,894. [16] 林君芬,吴梦娜,吴昊澄,等.浙江省新型冠状病毒肺炎病例流行特征分析[J].预防医学,2020,32(3):217-221,225. [17] 张栋梁,易波,陈奕,等.宁波市新型冠状病毒肺炎病例流行特征分析[J].预防医学,2020,32(4):330-333. [18] 曾希鹏,谢朝梅,谢燕湘,等.常德市79例新型冠状病毒肺炎确诊病例本底资料及核酸检测结果分析[J/OL].实用预防医学(2020-03-09)[2020-09-10].http://kns.cnki.net/kcms/detail/43.1223.r.20200306.1249.006.html. [19] CHARLTON C L,BABADY E,GINOCCHIO C C, et al.Practical guidance for clinical microbiology laboratories:viruses causing acute respiratory tract infections[J]. Clin Microbiol Rev,2018,32(1):1-49. [20] 莫茜,秦炜,傅启华,等.正确认识新型冠状病毒核酸检测的影响因素[J].中华检验医学杂志,2020,43(3):213-216. |
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