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| Dendrobium and Ganoderma lucidum oral liquid on immunity enhancement of mice |
| JIN Wei*, LEI Lin
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| * Sichuan Provincial Center for Disease Control and Prevention,Chengdu,Sichuan 610041,China |
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Abstract Objective To evaluate the immune function of Dendrobium and Ganoderma lucidum oral liquid in female KM mice. Methods A total of 160 healthy female KM mice were selected and divide into three dose groups and a control group. Dendrobium and Ganoderma lucidum oral liquid was administered to the mice in the three dose groups at 3.33,6.67 and 10.00 mL/kgbw by intragastric lavage for 30 days,while distilled water was given to the mice in the control group in the same way. The thymus and spleen were weighed and the visceral organ coefficients were calculated. The humoral immune function,cellular immune function,monocyte-macrophage phagocytosis function and natural killer（NK）cell activity were detected,then the effects of the oral liquid on immunity enhancement of mice could be proved with any two items above positive. Results No statistically significant differences were found between the dose groups and the control group in the visceral organ coefficients,serum hemolysis,lymphocyte proliferation ability,macrophage phagocytosis and NK cell activity（P>0.05）. In the middle- and high-dose group,the number of plague-forming cells per 106 spleen cells were 315.5±49.9 and 343.0±43.8,respectively,which were more than that in the control group（249.5±41.3,P<0.05）;the capacity of toe swelling in the middle- and high-dose group were（1.34±0.42）and（1.37±0.49）mm,respectively,which were more than that in the control group [（0.89±0.37）mm,P<0.05]. Conclusion Dendrobium and Ganoderma lucidum oral liquid have the effect of enhancing immunity on female KM mice.
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Received: 27 November 2018
Revised: 21 January 2019
Published: 13 May 2019
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