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| The threshold limit value research on N-methylcarbamoylated haemoglobin adducts |
| LU Yan-yan，XU Cheng-min，CHAI Jian-rong，WU Hao，PAN Ji，SUN Chuan，QIAN Ya-ling
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| Zhejiang Academy of Medical Sciences，Hangzhou，Zhejiang 310013，China |
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Abstract Objective To study the threshold limit value of N-methylcarbamoylated haemoglobin（NMHb）in the workers' blood exposed to N，N-dimethylformamide（DMF）in China. Methods The 168 workers and 30 administrative personals of the leather factory were chosen as the objects and the controls respectively. The ambient air measurements by personal air sampling at the factory were determined by gas chromatography. All the blood samples of the objects were collected and the concentrations of hemoglobin adducts in blood were determined by gas chromatography-mass spectrometry（GC/MS） . The objects all had occupational health examined. Through comparing the abnormal rate of liver function in the two groups，the correlation regression analysis of the DMF exposure concentration and the NMHb concentration in blood，the threshold limit value of NMHb occupational exposed to DMF was proposed. Results The DMF ambient concentrations of the leather factory were 0.100-648 mg/m3，and the NMHb concentrations of the homogeneity exposure group were 2.38-761 nmol/g Hb. The abnormal rate of liver function in homogeneous exposure group was 50.60%，which was higher than the control group （P<0.001） . The regression equation of the concentrations of NMHb in blood and the concentrations of DMF in the air was lg（NMHb）=0.552 lg（DMF）+1.319（r=0.862，P<0.01） . On the basis of the threshold limit value of DMF（30 mg/m3，ACGIH）and the regression equation，the concentrations of NMHb were calculated for 136.3 nmol/g Hb. The abnormal rate of liver function in low internal exposure group（NMHb ≤135 nmol/g Hb）was 42.39%，lower than 60.53% in high internal exposure group（NMHb>135 nmol/g Hb）（P<0.05） . In the exposure group（DMF<20 mg/m3），93.5% of the NMHb concentration of the occupational exposure workers was less than 135 nmol/g Hb. Conclusion The exposure concentration of DMF is correlated with abnormal liver function and the increasing NMHb concentration，and the threshold limit value of NMHb in the blood is recommended to be 135 nmol/g Hb.
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Published: 04 September 2018
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