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| A study on the concentration of sulfites in foods by online dialysis-ion chromatography method |
| WANG Yong-gen,WANG Jian-bo,BAO Ling-yue,ZHU Xiao-qiong
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| The Center for Disease Control and Prevention of Pinghu City,Pinghu,Zhejiang 314200,China |
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Abstract Objective To establish a rapid and accurate detecting method for sulfite in food residues.Methods Applying an fluorine ionsalkali-stable liquid to extract ultrasonically.After removing the suspended substances by high-speed centrifugation,we used online dialysis-ion chromatography to determine sulfite. Chromatographic conditionswere set as followsMetrosep A Supp 5-150 / 4.0 anion analysis column,using 3.2 mmol / L sodium carbonate -1.0 mmol / L sodium bicarbonate 5% acetone as the eluent,with 0.70 mL / min flow rate, 20 μL injection volume and a conductivity detector. Results Sulfites (calculated as sulfur dioxide) manifested a good linear relationship when its concentration ranged from 0.50 μg / mL to 50.00 μg / mL,r= 0.999 8.The minimum detection limit was 0.54 mg / kg. The spiked concentrations were 10.0 mg / kg, 100.0 mg / kg, 500.0 mg / kg, 900.0 mg / kg to samples. Relative standard deviation (n=6, %) was 5.04,1.20,0.56,0.98, with the recovery rate between 85.4% -104.4%. Conclusion This method is simple, rapid, with high sensitivity and accuracy of measurement, and which is suitable for the detection of sulfites content in food matrix.
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Received: 24 May 2016
Published: 21 November 2017
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