Abstract:Objective To evaluate the safety of the Eucommia folium and to provide the basis of the rational use of Eucommia folium resource. Methods According to < Technical Standards for Testing & Assessment of Health Food>,version 2003,acute safety test/micronucleus test of born marrow in mice/sperm shape abnormality test in mice/Ames test and thirty-day feeding test were all performed after water extract concentrations of Eucommia folium treatment(1 mL concentrations equal to 1.5 g Eucommia folium). Results On the acute toxicity test,the MTD of rats and mice were both 40.0 mL/kgbw. The results of genetic toxicity test were all negative in the dosage of 10 g/kg bw(according to the dosage of Eucommia folium).The results of thirty-day feeding test indicated that there have no significant differences in body weight,blood cytology indexes,blood biochemical indexes,organ/body ratio and pathematology examination in the rats in all the groups (0.83,1.67,3.30 mL/kg) as compared to the control group(P>0.05). Conclusion Under this experimental condition,no obvious abnormity and toxic reaction were observed.