Determination of pentachlorophenol in wooden chopping boards with gas chromatography-tandem mass spectrometry
ZHAO Yuwen1, LI Zeran2, XUE Xiangju1, YANG Meng1, JI Wenliang2, RONG Weiguang2
1. Department of Foods, Shandong Drug and Food Vocational College, Weihai, Shandong 264210, China; 2. Institute of Physical and Chemical Detection, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu 210009, China
Abstract：Objective To optimize the determination of pentachlorophenol in wooden chopping boards through pretreatment of miniaturized samples. Methods The pretreated wooden chopping board samples were subjected to ultrasound extraction (1 mL of 0.5 mol/L K2CO3 added in 5 mL extraction solution) in 8 mL acetone and 2 mL water, followed by derivatization with 0.3 mL acetic anhydride, extraction with n-hexane and separation with DB-5ms column (30 m×0.25 mm, 0.25 μm). Gas chromatography tandem mass spectrometry (GC-MS/MS) was performed in multiple reaction monitoring (MRM) mode with quantitative analysis using the internal standard method. Results The GC-MS/MS assay showed a good linear relationship within the range of 0.01 to 0.2 µg/mL (R2>0.999), with a 0.003 mg/kg limit of detection and 0.01 mg/kg limit of quantitation. The mean recovery rates were 84.2% to 96.7% at spiked concentrations of 0.003, 0.01 and 0.03 mg/kg, with relative standard deviation of 2.2% to 6.1%. Conclusions The established GC-MS/MS assay is easy to perform, environment-friendly, highly accurate and sensitivity, which is feasible for determination of pentachlorophenol in wooden chopping boards.
赵玉文, 李泽冉, 薛香菊, 杨萌, 吉文亮, 荣维广. 气相色谱串联质谱法检测木砧板中五氯苯酚[J]. 预防医学, 2023, 35(9): 825-828.
ZHAO Yuwen, LI Zeran, XUE Xiangju, YANG Meng, JI Wenliang, RONG Weiguang. Determination of pentachlorophenol in wooden chopping boards with gas chromatography-tandem mass spectrometry. Preventive Medicine, 2023, 35(9): 825-828.
