Research progress on approaches for detection of vitamin E in human blood
CHEN Yingqi1, XIN Jiarui1, HUANG Baifen2, HU Chonggao2, YANG Lei1
1. School of Public Health, Hangzhou Normal University, Hangzhou, Zhejiang 311121, China; 2. Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China
Abstract：There are eight forms of vitamin E in human blood, including α-, β-, γ-, δ-tocopherols and α-, β-, γ-, δ-tocotrienols. As the most abundant and active form of vitamin E, α-tocopherol is widely accepted as a reliable indicator for nutritional assessment of body vitamin E status across the world. Considering that different vitamin E forms have diverse biological activities, separation and detection of different vitamin E forms in human blood facilitates the understanding of the association between vitamin E and diseases. In this review, the advances in sample-pretreatment techniques and detection techniques for vitamin E in human blood were presented. Currently, the sample-pretreatment techniques include solid-phase extraction, liquid-liquid extraction, dispersive liquid-phase microextraction, supported liquid extraction and direct protein precipitation; the detection techniques include automatic biochemical analysis, enzyme-linked immunosorbent assay, gas chromatography, liquid chromatography and ultra-high performance supercritical fluid chromatography mass spectrometry. This review summarizes the characteristics and scope of above-mentioned techniques used for detection of vitamin E in human blood, so as to provide insights into the selection of an appropriate method for inspection technicians.
陈莹琦, 辛佳芮, 黄百芬, 胡崇高, 杨磊. 人体血液维生素E检测技术进展[J]. 预防医学, 2022, 34(1): 46-52.
CHEN Yingqi, XIN Jiarui, HUANG Baifen, HU Chonggao, YANG Lei. Research progress on approaches for detection of vitamin E in human blood. Preventive Medicine, 2022, 34(1): 46-52.
[1] JU J,PICINICH S C,YANG Z,et al.Cancer-preventive activities of tocopherols and tocotrienols[J].Carcinogenesis,2010,31(4):533-542. [2] YANG C S,LUO P,ZENG Z,et al.Vitamin E and cancer prevention:studies with different forms of tocopherols and tocotrienols[J].Mol Carcinog,2020,59(4):365-389. [3] PERVEZ M A,KHAN D A,SLEHRIA A U R,et al.Delta-tocotrienol supplementation improves biochemical markers of hepatocellular injury and steatosis in patients with nonalcoholic fatty liver disease:a randomized,placebo-controlled trial[J/OL].Complement Ther Med,2020,52[2021-11-10].https://doi.org/10.1016/j.ctim.2020.102494. [4] SHEN J J,YANG T,XU Y Z,et al.δ-tocotrienol,isolated from rice bran,exerts an anti-inflammatory effect via MAPKs and PPARs signaling pathways in lipopolysaccharide-stimulated macrophages[J/OL].Int J Mol Sci,2018,19(10)[2021-11-10]. https://doi.org/10.3390/ijms19103022. [5] KIM Y,WANG W,OKLA M,et al.Suppression of NLRP3 inflammasome by γ-tocotrienol ameliorates type 2 diabetes[J].J Lipid Res,2016,57(1):66-76. [6] CERVINKOVA B,KRCMOVA L K,SOLICHOVA D,et al.Recent advances in the determination of tocopherols in biological fluids:from sample pretreatment and liquid chromatography to clinical studies[J].Anal Bioanal Chem,2016,408(10):2407-2424. [7] ZHANG H,QUAN L,PEI P,et al.Simultaneous determination of vitamin A,25-hydroxyl vitamin D3 α-tocopherol in small biological fluids by liquid chromatography-tandem mass spectrometry[J].J Chromatogr B Analyt Technol Biomed Life Sci,2018,1079:1-8. [8] HASHEMI B,ZOHRABI P,SHAMSIPUR M.Recent developments and applications of different sorbents for SPE and SPME from biological samples[J].Talanta,2018,187:337-347. [9] KR-MOVÁ L,URBÁNEK L,SOLICHOVÁ D,et al.HPLC method for simultaneous determination of retinoids and tocopherols in human serum for monitoring of anticancer therapy[J].J Sep Sci,2009,32(15/16):2804-2811. [10] MATA-GRANADOS J M,QUESADA GÓMEZ J M,LUQUE DE CASTRO M D.Fully automatic method for the determination of fat soluble vitamins and vitamin D metabolites in serum[J].Clin Chim Acta,2009,403(1/2):126-130. [11] LEE M J,FENG W,YANG L,et al.Methods for efficient analysis of tocopherols,tocotrienols and their metabolites in animal samples with HPLC-EC[J].J Food Drug Anal,2018,26(1):318-329. [12] GIUSEPPONI D,GALARINI R,BAROLA C,et al.LC-MS/MS assay for the simultaneous determination of tocopherols,polyunsaturated fatty acids and their metabolites in human plasma and serum[J].Free Radic Biol Med,2019,144:134-143. [13] GIUSEPPONI D,TORQUATO P,BARTOLINI D,et al.Determination of tocopherols and their metabolites by liquid-chromatography coupled with tandem mass spectrometry in human plasma and serum[J].Talanta,2017,170:552-561. [14] MIDTTUN Ø,MCCANN A,AARSETH O,et al.Combined measurement of 6 fat-soluble vitamins and 26 water-soluble functional vitamin markers and amino acids in 50 μL of serum or plasma by high-throughput mass spectrometry[J].Anal Chem,2016,88(21):10427-10436. [15] PILA-OVÁ V,GOTTVALD T,SVOBODA P,et al.Development and optimization of ultra-high performance supercritical fluid chromatography mass spectrometry method for high-throughput determination of tocopherols and tocotrienols in human serum[J].Anal Chim Acta,2016,934:252-265. [16] RRZAEE M,YAMINI Y,FARAJI M.Evolution of dispersive liquid-liquid microextraction method[J].J Chromatogr A,2010,1217(16):2342-2357. [17] 聂宏骞,石慧,易长文,等.分散液液微萃取法在食品分析中应用的研究进展[J].职业与健康,2021,37(5):714-720. NIE H Q,SHI H,YI C W,et al.Research advancements of dispersive liquid-liquid microextraction in food analysis[J]. Occup Health,2021,37(5):714-720. [18] 张小敏,王洁,王淑静,等.DLPME-HPLC法同时测定人血清中维生素A和维生素E含量[J].医学信息,2019,32(20):161-163. ZHANG X M,WANG J,WANG S J,et al.Simultaneous determination of vitamin A and vitamin E in human serum by DLPME-HPLC[J]. Med Inf,2019,32(20):161-163. [19] CHIARA F,GIOVANNI D,SALVATORE F,et al.Advanced analytical techniques for fat-soluble vitamin analysis[J].Trends Analyt Chem,2017,87:82-97. [20] QI F F,TAO L M,DAI Y M,et al.Optimization and application of high-throughput supported liquid extraction for simultaneous determination of carotenoids and fat-soluble vitamins in serum[J/OL].J Chromatogr B Analyt Technol Biomed Life Sci,2021,1173(2021-03-24)[2021-11-10].https://doi.org/10.1016/j.jchromb.2021.122672. [21] PETRUZZIELLO F,GRAND-GUILLAUME PERRENOUD A,THORIMBERT A,et al.Quantitative profiling of endogenous fat-soluble vitamins and carotenoids in human plasma using an improved UHPSFC-ESI-MS interface[J].Anal Chem,2017,89(14):7615-7622. [22] PEERSMAN N,ELSLANDE J V,LEPAGE Y,et al.UPLC-MS/MS method for determination of retinol and α-tocopherol in serum using a simple sample pretreatment and UniSpray as ionization technique to reduce matrix effects[J].Clin Chem Lab Med,2020,58(5):769-779. [23] LE J,YUAN T F,ZHANG Y,et al.New LC-MS/MS method with single-step pretreatment analyzes fat-soluble vitamins in plasma and amniotic fluid[J].J Lipid Res,2018,59(9):1783-1790. [24] LAZZARINO G,LONGO S,AMORINI A M,et al.Single-step preparation of selected biological fluids for the high performance liquid chromatographic analysis of fat-soluble vitamins and antioxidants[J].J Chromatogr A,2017,1527:43-52. [25] 孔琳.浅谈自动生化分析仪的发展趋势及在医学检验中的应用[J].世界最新医学信息文摘,2015,15(59):29,32. KONG L. Introduction to the development trend of automatic biochemical analyzer and its application in medical test[J]. World Latest Med Inf,2015,15(59):29,32. [26] 阴春霞,肖贺丽,姜巍,等.妊娠早期孕妇血清中维生素A、D及E水平与早发型子痫前期的相关性[J].中国妇幼保健,2021,36(19):4418-4420. YIN C X,XIAO H L,JIANG W,et al.Vitamin A,D and E levels in serum in pregnant women in the early stages of pregnancy were associated with early hair pre-eclampsia[J]. Matern Child Health Care China,2021,36(19):4418-4420. [27] 郭冰杰,杨彩梅.子痫前期孕妇的血脂、血糖与维生素A、E的水平及其临床意义[J].广西医学,2017,39(10):1474-1476,1479. GUO B J,YANG C M.Expression levels and clinical significance of blood lipids,blood glucose,vitamin A and E in pregnant women with preeclanpsia[J]. Guangxi Med J,2017,39(10):1474-1476,1479. [28] 张伟利,陈瑞冠,任安林.气相色谱法测定人体血中维生素E[J].营养学报,1984,6(4):355-358. ZHANG W L,CHEN R G,REN A L.Gas chromatography measures vitamin E in human blood[J]. Acta Nutrimenta Sin,1984,6(4):355-358. [29] 谢宝英. 两种测定人血浆中维生素E方法的比较分析[J].中国现代药物应用,2021,15(14):234-237. XIE B Y.The comparative analysis of two determination method of vitamin E in human plasma[J]. Chin J Mod Drug Appl,2021,15(14):234-237. [30] 魏雪缘,沈伟健,张睿,等.食用植物油中生育酚和生育三烯酚检测方法的研究进展[J].食品安全质量检测学报,2016,7(2):686-692. WEI X Y,SHEN W J,ZHANG R,et al.Research progress on the determination methods of tocopherols and tocotrienols in edible vegetable oils[J]. Food Safe Qual Detec Technol,2016,7(2):686-692. [31] CHE H L,TAN D M,MEGANATHAN P,et al.Validation of a HPLC/FLD method for quantification of tocotrienols in human plasma[J].Int J Anal Chem,2015:1-7. [32] 赵莹,胡佳薇,赵静珺,等.高效液相色谱-多波长荧光检测法同时测定血清中维生素A和维生素E的含量[J].卫生研究,2020,49(4):585-590. ZHAO Y,HU J W,ZHAO J J,et al.Determination of vitamin A and vitamin E in human serum by high performance liquid chromatography with fluorescence detection[J]. J Hyg Res,2020,49(4):585-590. [33] CERVINKOVA B,KRCMOVA L K,KLABACKOVA S,et al.Rapid determination of lipophilic vitamins in human serum by ultra-high performance liquid chromatography using a fluorinated column and high-throughput miniaturized liquid-liquid extraction[J].J Sep Sci,2017,40(17):3375-3382. [34] 杨子辉. 食品安全分析中快速质谱方法及应用研究[D].长沙:湖南师范大学,2019. YANG Z H.Research on rapid mass spectrometry method and its application in food safety analysis[D].Changsha:Hunan Normal University,2019 [35] LUBIN A,GEERINCKX S,BAJIC S,et al.Enhanced performance for the analysis of prostaglandins and thromboxanes by liquid chromatography-tandem mass spectrometry using a new atmospheric pressure ionization source[J].J Chromatogr A,2016,1440:260-265. [36] HINCHLIFFE E,RUDGE J,REED P.A novel high-throughput method for supported liquid extraction of retinol and alpha-tocopherol from human serum and simultaneous quantitation by liquid chromatography tandem mass spectrometry[J].Ann Clin Biochem,2016,53(4):434-445. [37] 陈曦,宫照龙,沈葹.超高效液相色谱-三重四级杆串联质谱法测定血清中维生素A和维生素E[J].卫生研究,2021,50(2):301-307. CHEN X,GONG Z L,SHEN S.Determination of vitamin A and vitamin E in human serum by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry[J].J Hyg Res,2021,50(2):301-307. [38] ZHANG X,WEI W,TAO G,et al.Identification and quantification of triacylglycerols using ultraperformance supercritical fluid chromatography and quadrupole time-of-flight mass spectrometry:comparison of human milk,infant formula,other mammalian milk,and plant oil[J].J Agric Food Chem,2021,69(32):8991-9003.
