Abstract:Objective To examine the diagnostic and prognostic value of long non-coding RNA (lncRNA) JPX in mesothelioma, so as to provide insights into diagnosis and prognosis of mesothelioma. Methods Patients with clinically definitive diagnosis of mesothelioma from 2015 to 2019 that were sampled from asbestos processing plants in Zhejiang Province from 2015 to 2019 were recruited in the mesothelioma group, while healthy residents without asbestos exposure or asbestos-related diseases in the same area served as controls. Participants' demographics, pathologic diagnosis and imaging features were collected, and the expression of blood lncRNA JPX was detected using lncRNA microarrays. The diagnostic value of lncRNA JPX for mesothelioma was evaluated using the receiver operating characteristic (ROC) curve, and the correlation between lncRNA JPX expression and prognosis was examined among mesothelioma patients using survival analysis. Results There were 17 subjects in the mesothelioma group, with a mean age of (65.71±8.36) years, and 34 subjects in the controls, with a mean age of (64.24±8.70) years. LncRNA microarray detected significantly high lncRNA JPX expression in mesothelioma patients, and higher blood lncRNA JPX expression was detected in the mesothelioma group than in the control group [median (interquartile range), 1.10 (1.31) vs. 0.89 (0.54); t'=-2.300, P=0.034]. The area under the ROC curve was 0.673 (95%CI: 0.507-0.839, P=0.046), and if the cutoff was 1.759, the sensitivity and specificity were 35.3% and 100.0%, respectively. Survival analysis showed no significant difference in the survival rate of mesothelioma patients between the high lncRNA JPX expression group and the low expression group (χ2=0.212, P=0.645). Conclusions LncRNA JPX overexpression is detected in the blood of patients with mesothelioma, and lncRNA JPX expression presents a diagnostic value for mesothelioma; however, it shows little prognostic value for mesothelioma.
[1] CARBONE M,EMRI S,DOGAN A U,et al.A mesothelioma epidemic in Cappadocia:scientific developments and unexpected social outcomes[J].Nat Rev Cancer,2007,7(2):147-154. [2] CREANEY J,ROBINSON B W S.Malignant mesothelioma biomarkers:from discovery to use in clinical practice for diagnosis,monitoring,screening,and treatment[J].Chest,2017,152(1):143-149. [3] 沈蔚,刘佳琪,陈俊斐,等.恶性间皮瘤36例患者生存分析[J].预防医学,2022,34(1):66-69. [4] KOPP F,MENDELL J T.Functional classification and experimental dissection of long noncoding RNAs[J].Cell,2018,172(3):393-407. [5] BHAN A,SOLEIMANI M,MANDAL S S.Long noncoding RNA and cancer:a new paradigm[J].Cancer Res,2017,77(15):3965-3981. [6] SINGH A S,HEERY R,GRAY S G.In silico and in vitro analyses of lncRNAs as potential regulators in the transition from the epithelioid to sarcomatoid histotype of malignant pleural mesothelioma (MPM)[J/OL].Int J Mol Sci,2018,19(5)[2023-01-18].https://doi.org/10.3390/ijms19051297. [7] SUN S,DEL ROSARIO B C,SZANTO A,et al.JPX RNA activates Xist by evicting CTCF[J].Cell,2013,153(7):1537-1551. [8] YAO Y,CHEN S,LU N,et al.LncRNA JPX overexpressed in oral squamous cell carcinoma drives malignancy via miR-944/CDH2 axis[J].Oral Dis,2021,27(4):924-933. [9] HUSAIN A N,COLBY T V,ORDÓÑEZ N G,et al.Guidelines for pathologic diagnosis of malignant mesothelioma 2017 update of the consensus statement from the International Mesothelioma Interest Group[J].Arch Pathol Lab Med,2018,142(1):89-108. [10] 何权瀛,高莹慧.关于吸烟问题若干名词定义[J].中华结核和呼吸杂志,2009,32(1):56. [11] 房玥晖,何宇纳,白国银,等.2010—2012年中国成年女性居民饮酒行为现状及影响因素分析[J].中华流行病学杂志,2018,39(11):1432-1437. [12] BUENO R,STAWISKI E W,GOLDSTEIN L D,et al.Comprehensive genomic analysis of malignant pleural mesothelioma identifies recurrent mutations,gene fusions and splicing alterations[J].Nat Genet,2016,48(4):407-416. [13] WEBER D G,CASJENS S,BRIK A,et al.Circulating long non-coding RNA GAS5 (growth arrest-specific transcript 5)as a complement marker for the detection of malignant mesothelioma using liquid biopsies[J/OL].Biomark Res,2020,8[2023-01-18].https://doi.org/10.1186/s40364-020-00194-4. [14] MAO W,ZHANG X,GUO Z,et al.Association of asbestos exposure with malignant mesothelioma incidence in eastern China[J].JAMA Oncol,2017,3(4):562-564. [15] JIANG Z,SHEN W,YING S,et al.Overexpression of fibulin-3 in tumor tissue predicts poor survival of malignant mesothelioma patients from hand-spinning asbestos exposed area in eastern China[J/OL].Sci Rep,2020,10(1)[2023-01-18].https://doi.org/10.1038/s41598-020-77412-4.