Etiological characteristics of an outbreak of Campylobacter foodborne disease in a middle school
ZHENG Yanmin1, WANG Bo1, TENG Chengang1, HUANG Feifei1, TIAN Liqin1, YANG Haibing2, ZHANG Menghan2
1. Department of Food Hygiene, Suzhou Center for Disease Control and Prevention, Suzhou, Jiangsu 215004, China; 2. Suzhou Center for Disease Control and Prevention, Suzhou, Jiangsu 215004, China
Abstract:Objective To analyze the etiological characteristics of an outbreak of Campylobacter foodborne disease in a middle school in Suzhou City, so as to provide insights into the identification of pathogenic factors of Campylobacter foodborne disease outbreaks. Methods Eighteen anal swabs from patients, 10 anal swabs from canteen workers, 43 food samples, 2 drinking water samples, 2 food original material samples and 31 environmental samples were collected, and the pathogens were rapidly screened using the gastrointestinal infection detection strip. The pathogens were isolated and cultured using the double-pore filtration membrane method, and cluster analysis of bacterial isolates was performed using pulsed field gel electrophoresis ( PFGE ). In addition, the susceptibility of Campylobacter isolates to antibiotics was tested using the Campylobacter agar dilution method. Results A total of 63 cases with Campylobacter infections were reported, and the major clinical symptoms included diarrhea ( 51 cases, 80.95% ) and fever ( 39 cases, 61.90% ), while no inpatients or deaths were found. Twelve Campylobacter-positive samples were detected, including 11 anal swabs sampled from patients and one food original material sample. Among the 11 positive anal swabs, there were 10 samples positive for Campylobacter jejuni and one sample positive for C. coli, and of the one positive food original material, C. coli was identified. PFGE analysis showed that 10 C. jejuni isolates of had 100.0% homology, and these 10 isolates were 100.0% resistant to naphthyridic acid, ciprofloxacin and tetracycline, appearing multidrug resistance. Conclusions This is an outbreak of foodborne disease caused by C. jejuni infections. Gastrointestinal infection detection strips, double-pore filtration membrane and PFGE typing are rapid and accurate to identify pathogenic factors.
[1] HSIEH Y H,SULAIMAN I M.Chapter 5-Campylobacteriosis:an emerging infectious foodborne disease-science direct[J].Foodborne Dis,2018:119-155. [2] TÎRZIU E,BĂRBĂLAN,MORAR A,et al.Occurrence and antimicrobial susceptibility profile of Salmonella spp.in raw and ready-to-eat foods and Campylobacter spp.in retail raw chicken meat in Transylvania,Romania[J].Foodborne Pathog Dis,2020,17(8):479-484. [3] LI W,PIRES S M,LIU Z,et al.Surveillance of foodborne disease outbreaks in China,2003-2017[J/OL].Food Control,2020,118[2021-12-14].https://doi.org/10.1016/j.foodcont.2020.107359. [4] 刘敏,鞠长燕,马艳萍,等.深圳市南山区腹泻患者弯曲菌感染及病原学特征分析[J].疾病监测,2020,35(1):16-20. LIU M,JU C Y,MA Y P,et al.Infection status of Campylobacter and its etiologic characteristics in diarrhea patients in Nanshan district,Shenzhen[J].Dis Surveill,2020,35(1):16-20. [5] MOGHADAM S O,POURMAND M R,DOURAGHI M,et al.Utilization of PFGE as a powerful discriminative tool for the investigation of genetic diversity among MRSA strains[J].Iran J Public Health,2017,46(3):351-356. [6] 商晓春,周晓红,帅慧群,等.一起空肠弯曲菌食源性疾病突发事件的流行病学和病原学研究[J].中国学校卫生,2020,41(11):1741-1744. SHANG X C,ZHOU X H,SHUAI H Q,et al.Epidemiological and etiological study on an emergency of foodborne disease caused by Campylobacter jejuni[J].Chin J Sch Health,2020,41(11):1741-1744. [7] Clinical and Laboratory Standards Institute.Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria.3rd ed.CLSI Guideline M45[S].Wayne:Clinical and Laboratory Standards Institute,2015. [8] Centers for Disease Control and Prevention.Campylobacter[EB/OL].(2019-03-15)[2021-12-14].https://www.cdc.gov/narms/antibiotics-tested.html. [9] Centers for Disease Control and Prevention.Campylobacter[EB/OL].(2019-12-23)[2021-12-14].https://www.cdc.gov/campylobacter/faq.html. [10] LI Y,ZHANG S,HE M,et al.Prevalence and molecular characterization of Campylobacter spp.isolated from patients with diarrhea in Shunyi,Beijing[J/OL].Front Microbiol,2018,9[2021-12-14].https://doi.org/10.3389/fmicb.2018.00052. [11] ZHANG A,SONG L,LIANG H,et al.Molecular subtyping and erythromycin resistance of Campylobacter in China[J].J Appl Microbiol,2016,121(1):287-293. [12] 刘海波,刘颖,王云霞,等.2018年北京市房山区弯曲菌监测结果及耐药性分析[J].职业与健康,2020,36(9):1197-1199,1204. LIU H B,LIU Y,WANG Y X,et al.Surveillance results and antibiotic susceptibility analysis of Campylobacter in Fangshan District of Beijing in 2018[J].Occup Health,2020,36(9):1197-1199,1204. [13] YU H,ELBEDIWI M,ZHOU X H,et al.Epidemiological and genomic characterization of Campylobacter jejuni isolates from a foodborne outbreak at Hangzhou,China[J/OL].Int J Mol Sci,2020,21(8)[2021-12-14].https://doi.org/10.3390/ijms21083001.