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预防医学  2021, Vol. 33 Issue (9): 893-896    DOI: 10.19485/j.cnki.issn2096-5087.2021.09.007
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植物乳杆菌JX025073.1对小鼠血脂和肠道菌群的调节作用
张泽鹏, 郭展鹏, 倪钰玥, 富灿涯, 朱梦瑶, 付莹婕, 裘迪红
杭州医学院公共卫生学院,浙江 杭州 310053
Effect of Lactobacillus plantarum JX025073.1 on intestinal floraand blood lipid in mice
ZHANG Zepeng, GUO Zhanpeng, NI Yuyue, FU Canya, ZHU Mengyao, FU Yingjie, QIU Dihong
School of Public Health, Hangzhou Medical College, Hangzhou, Zhejiang 310053, China
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摘要 目的 了解植物乳杆菌JX025073.1对小鼠血脂及肠道菌群的调节作用,为益生菌用于高脂血症患者营养干预提供依据。方法 30只ICR雄性小鼠随机分为对照组、模型组和干预组,对照组喂基础饲料,模型组和干预组喂高脂饲料,干预组每天用0.4 mL植物乳杆菌JX025073.1发酵液灌胃,对照组和模型组用0.4 mL生理盐水灌胃。称量小鼠体重及心、肝、脾和肾重量,计算脏器指数;检测血清总胆固醇(TC)、三酰甘油(TG)和低密度脂蛋白胆固醇(LDL-C),以及肝和粪便中TC、TG;粪便双歧杆菌、乳杆菌和大肠杆菌培养和计数。结果 实验42 d后,模型组小鼠体重,血清TC、TG、LDL-C含量,肝和粪便TC、TG含量,以及大肠杆菌数量均高于对照组(P<0.05);粪便双歧杆菌、乳杆菌数量均低于对照组(P<0.05)。干预组小鼠体重,血清TC、TG、LDL-C含量,肝TC含量,以及粪便大肠杆菌数量均低于模型组(P<0.05);粪便TC、双歧杆菌和乳杆菌数量均高于模型组(P<0.05)。结论 植物乳杆菌JX025073.1能有效改善高脂饮食小鼠的血脂水平及肠道菌群结构。
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张泽鹏
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富灿涯
朱梦瑶
付莹婕
裘迪红
关键词 植物乳杆菌高脂饮食肠道菌群血脂    
AbstractObjective To learn the regulatory effect of Lactobacillus plantarum JX025073.1 on intestinal flora and blood lipid in mice, so as to provide the basis for the nutritional intervention of probiotics in hyperlipidemia population. Methods Thirty male ICR mice were randomly divided into a control group, a model group and a intervention group. The mice in the control group were fed with basic diet, and in the other two groups were fed with high fat diet. The mice in the intervention group was given 0.4 mL of Lactobacillus plantarum JX025073.1 fermentation liquid by gavage every day, and in the other two groups were given 0.4 mL of normal saline. The body weight of mice, the mass of heart, liver, spleen and kidney organs were weighed, and the organ index was calculated. The contents of total cholesterol (TC), triacylglycerol/triglyceride ( TG ), low density lipoprotein cholesterol ( LDL-C ) in serum, TC, TG in liver and in feces were determined by biochemical analyzer. Bifidobacterium, Lactobacillus and Escherichia coli in feces were cultured and counted. Results After 42 days, compared with the control group, The mice in the model group had higher body weights, higher levels of TC, TG and LDL-C in serum, higher levels of TC, TG in liver and in feces, more Escherichia coli, less Bifidobacterium and less Lactobacillus ( P<0.05 ). Compared with the model group, the mice in the intervention group had lower body weight, lower levels of TC, TG and LDL-C in serum, lower levels of TC in liver, less Escherichia coli, higher levels of TC in feces, more Bifidobacterium and more Lactobacillus ( P<0.05 ). Conclusion Lactobacillus plantarum JX025073.1 can effectively regulate the blood lipid and intestinal flora of mice fed with high fat diet.
Key wordsLactobacillus plantarum    high fat diet    intestinal flora    dyslipidemia
收稿日期: 2021-02-07      修回日期: 2021-05-23      出版日期: 2021-09-10
中图分类号:  R993  
基金资助:杭洲医学院创新创业项目
通信作者: 裘迪红,E-mail:371515334@qq.com   
作者简介: 张泽鹏,本科在读
引用本文:   
张泽鹏, 郭展鹏, 倪钰玥, 富灿涯, 朱梦瑶, 付莹婕, 裘迪红. 植物乳杆菌JX025073.1对小鼠血脂和肠道菌群的调节作用[J]. 预防医学, 2021, 33(9): 893-896.
ZHANG Zepeng, GUO Zhanpeng, NI Yuyue, FU Canya, ZHU Mengyao, FU Yingjie, QIU Dihong. Effect of Lactobacillus plantarum JX025073.1 on intestinal floraand blood lipid in mice. Preventive Medicine, 2021, 33(9): 893-896.
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