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预防医学  2018, Vol. 30 Issue (4): 370-373    DOI: 10.19485/j.cnki.issn2096-5087.2018.04.013
  论著 本期目录 | 过刊浏览 | 高级检索 |
台州市健康体检人群MTHFR C677T基因和MTHFR A1298C基因多态性研究
顾婉红,王攀,钟倩怡,杨英梅
台州市中心医院,浙江 台州 318000
Polymorphism of MTHFR C677T gene and MTHFR A1298C gene among physical examinees in Taizhou City
GU Wan-hong,WANG Pan,ZHONG Qian-yi,YANG Ying-mei
Taizhou Central Hosptial,Taizhou,Zhejiang 318000,China
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摘要 目的 了解台州市健康体检人群亚甲基四氢叶酸还原酶(MTHFR)C677T基因和MTHFR A1298C基因多态性的分布。方法 以2016年9月—2017年9月在台州市中心医院进行健康体检者为研究对象,采集血液应用荧光定量PCR法检测MTHFR C677T以及MTHFR A1298C基因型,比较不同性别和地域人群MTHFR C677T和MTHFR A1298C等位基因和基因型频率分布差异。结果 体检人群MTHFR 677CC、677CT、677TT基因型频率分别为40.09%、44.53%和15.39%,677T等位基因频率为37.65%;MTHFR 1298AA、1298AC、1298CC基因型频率分别为65.69%、30.47%和3.83%,1298C等位基因频率为19.07%。男女性体检者的MTHFR C677T基因型分布差异有统计学意义(P=0.036),但MTHFR A1298C基因型分布差异无统计学意义(P=0.278)。台州市健康体检人群MTHFR C677T等位基因和基因型频率分布情况与河南、乌鲁木齐和北京比较,差异均有统计学意义(P<0.05);与武汉比较,差异均无统计学意义(P>0.05)。结论 台州市健康体检人群MTHFR C677T基因多态性分布受性别影响,且具有一定的地域特点。
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顾婉红
王攀
钟倩怡
杨英梅
关键词 亚甲基四氢叶酸还原酶基因多态性体检人群地域    
AbstractObjective To explore the distribution of methylenetetrahydrofolate reductase(MTHFR)C677T gene and MTHFR A1298C gene polymorphism among 1 644 physical examinees in Taizhou region.Methods Fluorescence quantitative PCR method was used to detect MTHFR C677T and MTHFR A1298C genotype in 1 644 physical examinees from Taizhou Central Hospital from September 2016 to September 2017. According to the characteristics of gender,the distribution features of MTHFR C677T and MTHFR A1298C genotype were described,and then were compared with data about the physical examinees in other cities in China.Results Among the 1 644 physical examinees,MTHFR 677CC,677CT and 677TT genotype frequency were 40.09%,44.53%and 15.39% respectively and MTHFR 677 allele frequency was 37.65%. MTHFR 1298AA,1298AC and 1298CC genotype frequency were 65.69%,30.47% and 3.83% respectively and MTHFR 1298 allele frequency was 19.07%. Statistical significance was found in genotype distribution of MTHFR C677T between males and females(P=0.036), and no statistical significance was found in genotype distribution of MTHFR A1298C between males and females(P=0.278). As compared with the physical examinees in Henan,Wulumuqi and Beijing regions,there were statistically significance differences in the distribution of MTHFR C677T genotype and allele frequencies in Taizhou region(P<0.05).Conclusion The distribution of MTHFR C677T gene polymorphism among physical examinees in Taizhou region is affected by gender,and the results shows certain regional specificity.
Key wordsMethylenetetrahydrofolate reductase    Gene polymorphism    Physical examinees    Regional specificity
          出版日期: 2018-03-26
中图分类号:  R394  
通信作者: 顾婉红,E-mail:guwh8282@tzzxyy.com   
作者简介: 顾婉红,本科,检验师,主要从事免疫和分子检验工作
引用本文:   
顾婉红, 王攀, 钟倩怡, 杨英梅. 台州市健康体检人群MTHFR C677T基因和MTHFR A1298C基因多态性研究[J]. 预防医学, 2018, 30(4): 370-373.
GU Wan-hong, WANG Pan, ZHONG Qian-yi, YANG Ying-mei. Polymorphism of MTHFR C677T gene and MTHFR A1298C gene among physical examinees in Taizhou City. Preventive Medicine, 2018, 30(4): 370-373.
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http://www.zjyfyxzz.com/CN/10.19485/j.cnki.issn2096-5087.2018.04.013      或      http://www.zjyfyxzz.com/CN/Y2018/V30/I4/370
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