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预防医学  2021, Vol. 33 Issue (11): 1109-1112    DOI: 10.19485/j.cnki.issn2096-5087.2021.11.007
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5-HMF对早期2型糖尿病合并肝损伤小鼠糖脂代谢和肝功能影响的研究
鹿伟, 夏勇, 张世鑫, 宋燕华, 蔡德雷, 徐彩菊, 赵李丽
浙江省疾病预防控制中心理化与毒理检验所,浙江 杭州 310051
Effects of 5-HMF on glycolipid metabolism and hepatic function in mice with diabetes and hepatic injury
LU Wei, XIA Yong, ZHANG Shixin, SONG Yanhua, CAI Delei, XU Caiju, ZHAO Lili
Department of Physicochemical and Toxicology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China
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摘要 目的 研究5-羟甲基-2-糠醛(5-HMF)对早期2型糖尿病(T2DM)合并肝损伤小鼠糖脂代谢和肝功能的影响。方法 设5-HMF低、中、高剂量组,模型对照组和正常对照组,每组10只雌性ICR小鼠。低、中、高剂量组每天按10 mL/kg体重分别给予0.27、0.80、2.67 mg/kg 5-HMF灌胃,模型对照组和正常对照组给予同体积去离子水,连续12周。各剂量组和模型对照组喂饲36%高脂高糖饲料,实验第10、11周腹腔注射四氧嘧啶60 mg/kg体重;正常对照组喂饲维持饲料。定期测定体重、血糖、血脂和肝功能。留取肝组织,肝糖原高碘酸-希夫染色,观察组织病理学变化。结果 模型对照组小鼠血糖(GLU)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、谷丙转氨酶(ALT)、谷草转氨酶(AST)和乳酸脱氢酶(LDH)水平均高于正常对照组(P<0.05)。低、高剂量组小鼠AST水平,低、中、高剂量组小鼠LDH水平均低于模型对照组(P<0.05)。低、中、高剂量组小鼠GLU、总胆固醇(TC)、三酰甘油(TG)、LDL-C、HDL-C和ALT水平与模型对照组比较差异均无统计学意义(P>0.05)。模型对照组小鼠肝组织可见中至重度肝细胞空泡变性,高剂量组小鼠仅见轻微至轻度空泡变性;高剂量组和模型对照组小鼠均有中等或大量肝糖原颗粒。结论 本实验条件下尚不能判断5-HMF对T2DM合并肝损伤小鼠有降血糖和降血脂作用,但可证明其对小鼠肝组织及功能有保护作用。
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鹿伟
夏勇
张世鑫
宋燕华
蔡德雷
徐彩菊
赵李丽
关键词 5-羟甲基-2-糠醛2型糖尿病肝损伤血糖血脂肝功能    
AbstractObjective To observe the effect of 5-hudroxymethyl-2-furfural (5-HMF) on glycolipid metabolism and hepatic function in mice with type 2 diabetes mellitus (T2DM) and hepatic injury. Methods A low, a medium and a high 5-HMF dose group, a model group, and a control group were designed, with ten female ICR mice in each group. The low, medium and high dose group were given 0.27, 0.80 and 2.67 mg/kgbw 5-HMF, respectively, for 12 weeks; while the model group and the control group were given volume controlled deionized water. The model group and three dose groups were fed with high-fat and high-sugar food (36%), and the intraperitoneal injection of alloxan (60 mg/kgbw) was executed in the 10th and 11th week; the control group were fed with normal food. The body weight, blood glucose, blood lipid, and liver function of mice were determined regularly. The livers were stained by periodic acid Schiff and the changes in pathology were observed. Results Compared with the control group, the serum levels of glucose (GLU), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) were significantly higher in the model group (P<0.05). Compared with the model group, the AST level in the low and high 5-HMF dose group, and the LDH level in the low, medium and high 5-HMF dose group, were significantly lower (P<0.05). There were no significant differences in the levels of GLU, total cholesterol, LDL-C, triacylglycerol, HDL-C and ALT between the model group and the three dose groups (P>0.05). Moderate to severe vacuolar degeneration was observed in the model group, while mild vacuolar degeneration was observed in the high dose group. Medium or large amount of hepatic glycogen granules were observed in the high dose group and the model group. Conclusion Under the conditions of this experiment, 5-HMF does not show any obvious function of reducing blood glucose and lipid in the mice with T2DM and liver injury, but show some protective effects on liver function.
Key words5-hudroxymethyl-2-furfural    type 2 diabetes mellitus    hepatic injury    blood glucose    blood lipid    hepatic function
收稿日期: 2021-06-30      修回日期: 2021-09-10      出版日期: 2021-11-10
中图分类号:  R992  
通信作者: 徐彩菊,E-mail:cjxu@cdc.zj.cn   
作者简介: 鹿伟,硕士,主管医师,主要从事卫生毒理学研究工作
引用本文:   
鹿伟, 夏勇, 张世鑫, 宋燕华, 蔡德雷, 徐彩菊, 赵李丽. 5-HMF对早期2型糖尿病合并肝损伤小鼠糖脂代谢和肝功能影响的研究[J]. 预防医学, 2021, 33(11): 1109-1112.
LU Wei, XIA Yong, ZHANG Shixin, SONG Yanhua, CAI Delei, XU Caiju, ZHAO Lili. Effects of 5-HMF on glycolipid metabolism and hepatic function in mice with diabetes and hepatic injury. Preventive Medicine, 2021, 33(11): 1109-1112.
链接本文:  
http://www.zjyfyxzz.com/CN/10.19485/j.cnki.issn2096-5087.2021.11.007      或      http://www.zjyfyxzz.com/CN/Y2021/V33/I11/1109
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