Abstract：Objective To investigate the detection of natural infection with pathogens in rodents in Huzhou City, Zhejiang Province, so as to provide a basis for risk assessment and control of rodent-borne diseases. Methods From August to September each year during the period of 2022-2024, urban residential areas, rural residential areas, key industries, farmland and cultivated land, forests, and shrublands in various counties (districts) of Huzhou City were selected as rodent surveillance sites. Rodents were captured using the trap night method. Following anesthesia, the rodents were euthanized by cervical dislocation. Species were identified based on morphological characteristics. Under sterile conditions, dissection was performed to collect liver, spleen, lung, and kidney tissues. These tissues were then mixed together, and detected for Hantavirus, Dabie bandavirus, Leptospira, and Orientia tsutsugamushi using fluorescence quantitative PCR. The detection rates of these natural infections with pathogens were analyzed across different rodent species, counties (districts), and habitats. Results A total of 259 rodents were captured from 2022 to 2024, including Rattus norvegicus, Rattus tanezumi, Suncus murinus, Mus musculus, Leopoldamys edwardsi, Niviventer fulvescens, Rattus losea, and Apodemus agrarius. The dominant species were Rattus norvegicus (142 individuals) and Rattus tanezumi (59 individuals), collectively accounting for 77.61%. With the exception of Orientia tsutsugamushi, Hantavirus, Dabie bandavirus, and Leptospira were all detected, with detection rates of 5.02%, 1.93%, and 10.42%, respectively. Hantavirus and Leptospira were simultaneously detected in one Rattus norvegicus, yielding a mixed infection rate of 0.39%. The three pathogens were detected in rodents in Wuxing District, Nanxun District, Deqing County, Changxing County, and Anji County, with detection rates of 6.45%, 5.71%, 26.32%, 4.76%, and 24.00%, respectively. They were also detected in rodents in urban residential areas, rural residential areas, key industries, farmland, and forests, with detection rates of 28.57%, 21.57%, 10.91%, 12.50%, and 14.81%, respectively. Notably, all three pathogens were simultaneously detected in Rattus norvegicus, Rattus tanezumi, rural residential areas, and key industries. ConclusionsRattus norvegicus and Rattus tanezumi were identified as the dominant rodent species in Huzhou City. The detection of Hantavirus, Dabie bandavirus, and Leptospira, including instances of mixed infection. It is necessary to enhance surveillance and control measures targeting these key species and high-risk habitats.
[1] HAN B A,KRAMER A M,DRAKE J M.Global patterns of zoonotic disease in mammals[J].Trends Parasitol,2016,32(7):565-577. [2] 龚震宇. 浙江省病媒生物监测工作进展[J].预防医学,2021,33(11):1081-1085. GONG Z Y.Progress of vector surveillance in Zhejiang Province[J].China Prev Med J,2021,33(11):1081-1085.(in Chinese) [3] 苏帆,孙继民,凌锋,等.浙江省2021年肾综合征出血热流行特征及宿主动物监测分析[J].中国媒介生物学及控制杂志,2023,34(3):378-382. SU F,SUN J M,LING F,et al.Epidemiological characteristics of hemorrhagic fever with renal syndrome and host animal surveillance in Zhejiang Province,China,2021[J].Chin J Vector Biol Control,2023,34(3):378-382.(in Chinese) [4] 郭颂,姚文武,刘营,等.2023年浙江省钩端螺旋体病监测分析[J].中华地方病学杂志,2024,43(7):559-563. GUO S,YAO W W,LIU Y,et al.Surveillance and analysis of leptospirosis in Zhejiang Province in 2023[J].Chin J Endem,2024,43(7):559-563.(in Chinese) [5] 施旭光,孙继民,刘营,等.2015—2019年浙江省发热伴血小板减少综合征流行特征分析[J].疾病监测,2021,36(5):431-435. SHI X G,SUN J M,LIU Y,et al.Epidemiological characteristics of fever with thrombocytopenia syndrome in Zhejiang,2015-2019[J].Dis Surveill,2021,36(5):431-435.(in Chinese) [6] 吴瑜燕,龚震宇,侯娟,等.浙江省2011—2013年病媒生物监测结果分析[J].中国媒介生物学及控制杂志,2015,26(4):394-397. WU Y Y,GONG Z Y,HOU J,et al.Analysis of vector surveillance from 2011 to 2013 in Zhejiang Province,China[J].Chin J Vector Biol Control,2015,26(4):394-397.(in Chinese) [7] 明小力,杨晓庆,周瑜,等.我国夏季型恙虫病流行病学研究进展[J].预防医学,2023,35(9):778-780,785. MING X L,YANG X Q,ZHOU Y,et al.Research progress on the epidemiology of tsutsugamushi disease of summer type in China[J].China Prev Med J,2023,35(9):778-780,785.(in Chinese) [8] SCHMIDT S,ESSBAUER S S,MAYER-SCHOLL A,et al.Multiple infections of rodents with zoonotic pathogens in Austria[J].Vector Borne Zoonotic Dis,2014,14(7):467-475. [9] TELFER S,LAMBIN X,BIRTLES R,et al.Species interactions in a parasite community drive infection risk in a wildlife population[J].Science,2010,330(6001):243-246. [10] 栗冬梅,李瑞晓,刘起勇.2021年全国病媒生物鼠传病原监测报告[J].中国媒介生物学及控制杂志,2023,34(1):1-8. LI D M,LI R X,LIU Q Y.National surveillance report on rodent-borne pathogens of disease vectors in 2021[J].Chin J Vector Biol Control,2023,34(1):1-8.(in Chinese) [11] 赵怡双,吴瑜燕,周海慧,等.浙江省台州市农村地区鼠形动物自然感染4种病原体的调查[J].疾病监测,2021,36(9):894-897. ZHAO Y S,WU Y Y,ZHOU H H,et al.Investigation of natural infection of four pathogens in rodents in rural area of Taizhou,Zhejiang[J].Dis Surveill,2021,36(9):894-897.(in Chinese) [12] 张亚萍,王文英,李莉莉,等.我国肾综合征出血热流行病学特征及预防控制研究现状[J].中华卫生杀虫药械,2020,26(4):387-393. ZHANG Y P,WANG W Y,LI L L,et al.Research status of epidemiology and prevention and control of hemorrhagic fever with renal syndrome in China[J].Chin J Hyg Insectic Equip,2020,26(4):387-393.(in Chinese) [13] 宋富成,修璟威,王伟,等.2017—2021年青岛市国家级鼠类监测点监测报告[J].中国媒介生物学及控制杂志,2022,33(5):696-700. SONG F C,XIU J W,WANG W,et al.Surveillance report on rodents at national rodent surveillance sites of Qingdao,China,2017-2021[J].Chin J Vector Biol Control,2022,33(5):696-700.(in Chinese) [14] 李克,庞志峰,吴晓虹,等.2007—2024年金华市钩端螺旋体病流行特征分析[J].预防医学,2025,37(8):818-821. LI K,PANG Z F,WU X H,et al.Epidemiological characteristics of leptospirosis in Jinhua City from 2007 to 2024[J].China Prev Med J,2025,37(8):818-821.(in Chinese) [15] BARRAGAN V,OLIVAS S,KEIM P,et al.Critical knowledge gaps in our understanding of environmental cycling and transmission of leptospira spp[J].Appl Environ Microbiol,2017,83(19):1-10.
