Research progress on approaches for detection of vitamin E in human blood
CHEN Yingqi1, XIN Jiarui1, HUANG Baifen2, HU Chonggao2, YANG Lei1
1. School of Public Health, Hangzhou Normal University, Hangzhou, Zhejiang 311121, China; 2. Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China
Abstract:There are eight forms of vitamin E in human blood, including α-, β-, γ-, δ-tocopherols and α-, β-, γ-, δ-tocotrienols. As the most abundant and active form of vitamin E, α-tocopherol is widely accepted as a reliable indicator for nutritional assessment of body vitamin E status across the world. Considering that different vitamin E forms have diverse biological activities, separation and detection of different vitamin E forms in human blood facilitates the understanding of the association between vitamin E and diseases. In this review, the advances in sample-pretreatment techniques and detection techniques for vitamin E in human blood were presented. Currently, the sample-pretreatment techniques include solid-phase extraction, liquid-liquid extraction, dispersive liquid-phase microextraction, supported liquid extraction and direct protein precipitation; the detection techniques include automatic biochemical analysis, enzyme-linked immunosorbent assay, gas chromatography, liquid chromatography and ultra-high performance supercritical fluid chromatography mass spectrometry. This review summarizes the characteristics and scope of above-mentioned techniques used for detection of vitamin E in human blood, so as to provide insights into the selection of an appropriate method for inspection technicians.
陈莹琦, 辛佳芮, 黄百芬, 胡崇高, 杨磊. 人体血液维生素E检测技术进展[J]. 预防医学, 2022, 34(1): 46-52.
CHEN Yingqi, XIN Jiarui, HUANG Baifen, HU Chonggao, YANG Lei. Research progress on approaches for detection of vitamin E in human blood. Preventive Medicine, 2022, 34(1): 46-52.
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