Abstract:Objective To establish a high performance liquid chromatography(HPLC)through the optimization of the chromatographic conditions,which can detect the contents of clenbuterol hydrochloride(CL)residues in animal edible product in a large quantity. Methods The animal edible product were extracted by perchloric acid,and then impurities were removed by liquid-liquid extraction(LLE)which used ethyl acetate- isopropanol. After the organic phase was concentrated,C18 column(150 mm×4.6 mm,5 μm)was used to separate CL. Mobile phase were methanol-sodium dihydrogen phosphate,and then determined by HPLC. Results A good linear response was obtained over the range of 0.2-10.0 μg/mL with the correlation coefficient(r)0.999 84. The method determination limit was 0.15 μg/kg which was lower than the National standard method 0.5 μg/kg. The retention time of the CL was 6.51 min,the chromatographic peak was good. The recovery rates spiked with standards 1.6-12 μg were 92.86%-100.93%,which was higher than National standard method(89.79%-92.36%). The precision of intra-day and inter-day were both under 5%,which lower than National standard. Conclusion The optimized chromatographic conditions are suitable for the large quantity determination of clenbuterol hydrochloride in animal edible product.